Gut microbiota related to Giardia duodenalis, Entamoeba spp. and Blastocystis hominis infections in humans from Côte d’Ivoire
Introduction: Literature data provide little information about protozoa infections and gut microbiota compositional shifts in humans. This preliminary study aimed to describe the fecal bacterial community composition of people from Côte d’Ivoire harboring Giardia duodenalis, Entamoeba spp., and Blas...
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| Format: | Article |
| Language: | English |
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The Journal of Infection in Developing Countries
2016-09-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/8179 |
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| author | Valerio Iebba Floriana Santangelo Valentina Totino Fabrizio Pantanella Anatole Monsia Veronica Di Cristanziano David Di Cave Serena Schippa Federica Berrilli Rossella D'Alfonso |
| author_facet | Valerio Iebba Floriana Santangelo Valentina Totino Fabrizio Pantanella Anatole Monsia Veronica Di Cristanziano David Di Cave Serena Schippa Federica Berrilli Rossella D'Alfonso |
| author_sort | Valerio Iebba |
| collection | DOAJ |
| description | Introduction: Literature data provide little information about protozoa infections and gut microbiota compositional shifts in humans. This preliminary study aimed to describe the fecal bacterial community composition of people from Côte d’Ivoire harboring Giardia duodenalis, Entamoeba spp., and Blastocystis hominis, in trying to discover possible alterations in their fecal microbiota structure related to the presence of such parasites.
Methodology: Twenty fecal samples were collected from people inhabiting three different localities of Côte d’Ivoire for copromicroscopic analysis and molecular identification of G. duodenalis, Entamoeba spp., and B. hominis. Temporal temperature gradient gel electrophoresis (TTGE) was used to obtain a fingerprint of the overall bacterial community; quantitative polymerase chain reaction (qPCR) was used to define the relative abundances of selected bacterial species/group, and multivariate statistical analyses were employed to correlate all data.
Results: Cluster analysis revealed a significant separation of TTGE profiles into four clusters (p < 0.0001), with a marked difference for G. duodenalis-positive samples in relation to the others (p = 5.4×10-6). Interestingly, qPCR data showed how G. duodenalis-positive samples were related to a dysbiotic condition that favors potentially harmful species (such as Escherichia coli), while Entamoeba spp./B. hominis-positive subjects were linked to a eubiotic condition, as shown by a significantly higher Faecalibacterium prausnitzii-Escherichia coli ratio.
Conclusions: This preliminary investigation demonstrates a differential fecal microbiota structure in subjects infected with G. duodenalis or Entamoeba spp./B. hominis, paving the way for using further next-generation DNA technologies to better understand host-parasite-bacteria interactions, aimed at identifying potential indicators of microbiota changes.
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| format | Article |
| id | doaj-art-06de35d8b3d64ffc8a645591a6633aee |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2016-09-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-06de35d8b3d64ffc8a645591a6633aee2025-08-20T02:27:11ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802016-09-01100910.3855/jidc.8179Gut microbiota related to Giardia duodenalis, Entamoeba spp. and Blastocystis hominis infections in humans from Côte d’IvoireValerio Iebba0Floriana Santangelo1Valentina Totino2Fabrizio Pantanella3Anatole Monsia4Veronica Di Cristanziano5David Di Cave6Serena Schippa7Federica Berrilli8Rossella D'Alfonso9Sapienza University of Rome, Rome, ItalySapienza University of Rome, Rome, ItalySapienza University of Rome, Rome, ItalySapienza University of Rome, Rome, ItalyCentre Don Orione pour handicapés physiques, Bonoua, Côte d’IvoireUniversity of Cologne, Cologne, GermanyUniversity of Rome Tor Vergata, Rome, ItalySapienza University of Rome, Rome, ItalyUniversity of Rome Tor Vergata, Rome, ItalyCentre Don Orione pour handicapés physiques, Bonoua, Côte d’IvoireIntroduction: Literature data provide little information about protozoa infections and gut microbiota compositional shifts in humans. This preliminary study aimed to describe the fecal bacterial community composition of people from Côte d’Ivoire harboring Giardia duodenalis, Entamoeba spp., and Blastocystis hominis, in trying to discover possible alterations in their fecal microbiota structure related to the presence of such parasites. Methodology: Twenty fecal samples were collected from people inhabiting three different localities of Côte d’Ivoire for copromicroscopic analysis and molecular identification of G. duodenalis, Entamoeba spp., and B. hominis. Temporal temperature gradient gel electrophoresis (TTGE) was used to obtain a fingerprint of the overall bacterial community; quantitative polymerase chain reaction (qPCR) was used to define the relative abundances of selected bacterial species/group, and multivariate statistical analyses were employed to correlate all data. Results: Cluster analysis revealed a significant separation of TTGE profiles into four clusters (p < 0.0001), with a marked difference for G. duodenalis-positive samples in relation to the others (p = 5.4×10-6). Interestingly, qPCR data showed how G. duodenalis-positive samples were related to a dysbiotic condition that favors potentially harmful species (such as Escherichia coli), while Entamoeba spp./B. hominis-positive subjects were linked to a eubiotic condition, as shown by a significantly higher Faecalibacterium prausnitzii-Escherichia coli ratio. Conclusions: This preliminary investigation demonstrates a differential fecal microbiota structure in subjects infected with G. duodenalis or Entamoeba spp./B. hominis, paving the way for using further next-generation DNA technologies to better understand host-parasite-bacteria interactions, aimed at identifying potential indicators of microbiota changes. https://jidc.org/index.php/journal/article/view/8179microbiotaGiardia duodenalisEntamoeba spp.Blastocystis hominisdysbiosisCôte d’Ivoire |
| spellingShingle | Valerio Iebba Floriana Santangelo Valentina Totino Fabrizio Pantanella Anatole Monsia Veronica Di Cristanziano David Di Cave Serena Schippa Federica Berrilli Rossella D'Alfonso Gut microbiota related to Giardia duodenalis, Entamoeba spp. and Blastocystis hominis infections in humans from Côte d’Ivoire Journal of Infection in Developing Countries microbiota Giardia duodenalis Entamoeba spp. Blastocystis hominis dysbiosis Côte d’Ivoire |
| title | Gut microbiota related to Giardia duodenalis, Entamoeba spp. and Blastocystis hominis infections in humans from Côte d’Ivoire |
| title_full | Gut microbiota related to Giardia duodenalis, Entamoeba spp. and Blastocystis hominis infections in humans from Côte d’Ivoire |
| title_fullStr | Gut microbiota related to Giardia duodenalis, Entamoeba spp. and Blastocystis hominis infections in humans from Côte d’Ivoire |
| title_full_unstemmed | Gut microbiota related to Giardia duodenalis, Entamoeba spp. and Blastocystis hominis infections in humans from Côte d’Ivoire |
| title_short | Gut microbiota related to Giardia duodenalis, Entamoeba spp. and Blastocystis hominis infections in humans from Côte d’Ivoire |
| title_sort | gut microbiota related to giardia duodenalis entamoeba spp and blastocystis hominis infections in humans from cote d ivoire |
| topic | microbiota Giardia duodenalis Entamoeba spp. Blastocystis hominis dysbiosis Côte d’Ivoire |
| url | https://jidc.org/index.php/journal/article/view/8179 |
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