A streamlined tandem affinity purification of His-MBP-SpyCas9, without buffer exchange, suitable for in vitro cleavage applications

A streamlined tandem affinity purification of His-MBP-SpyCas9, without buffer exchange, suitable for in vitro cleavage applications

We present a streamlined protocol for the purification of recombinant doubly-tagged His-MBP-SpyCas9 protein utilizing a dual affinity chromatography approach whereby the elution volume from immobilized metal ion affinity chromatography (IMAC) is loaded directly onto maltose-binding protein (MBP) aff...

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Bibliographic Details
Main Authors: Filippo Fronza, Roberto Verardo, Claudio Schneider
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:MethodsX
Subjects:
Cas9 tandem affinity purification
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016125002146
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