The Cytotoxic Role of Intermittent High Glucose on Apoptosis and Cell Viability in Pancreatic Beta Cells
Objectives. Glucose fluctuations are both strong predictor of diabetic complications and crucial factor for beta cell damages. Here we investigated the effect of intermittent high glucose (IHG) on both cell apoptosis and proliferation activity in INS-1 cells and the potential mechanisms. Methods. Ce...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2014/712781 |
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| author | Zhen Zhang Jing Li Lei Yang Rongping Chen Rui Yang Hua Zhang Dehong Cai Hong Chen |
| author_facet | Zhen Zhang Jing Li Lei Yang Rongping Chen Rui Yang Hua Zhang Dehong Cai Hong Chen |
| author_sort | Zhen Zhang |
| collection | DOAJ |
| description | Objectives. Glucose fluctuations are both strong predictor of diabetic complications and crucial factor for beta cell damages. Here we investigated the effect of intermittent high glucose (IHG) on both cell apoptosis and proliferation activity in INS-1 cells and the potential mechanisms. Methods. Cells were treated with normal glucose (5.5 mmol/L), constant high glucose (CHG) (25 mmol/L), and IHG (rotation per 24 h in 11.1 or 25 mmol/L) for 7 days. Reactive oxygen species (ROS), xanthine oxidase (XOD) level, apoptosis, cell viability, cell cycle, and expression of cyclinD1, p21, p27, and Skp2 were determined. Results. We found that IHG induced more significant apoptosis than CHG and normal glucose; intracellular ROS and XOD levels were more markedly increased in cells exposed to IHG. Cells treated with IHG showed significant decreased cell viability and increased cell proportion in G0/G1 phase. Cell cycle related proteins such as cyclinD1 and Skp2 were decreased significantly, but expressions of p27 and p21 were increased markedly. Conclusions. This study suggested that IHG plays a more toxic effect including both apoptosis-inducing and antiproliferative effects on INS-1 cells. Excessive activation of cellular stress and regulation of cyclins might be potential mechanism of impairment in INS-1 cells induced by IHG. |
| format | Article |
| id | doaj-art-06c0bd88fb3c4e60b33bbea28adce68e |
| institution | OA Journals |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-06c0bd88fb3c4e60b33bbea28adce68e2025-08-20T02:02:50ZengWileyJournal of Diabetes Research2314-67452314-67532014-01-01201410.1155/2014/712781712781The Cytotoxic Role of Intermittent High Glucose on Apoptosis and Cell Viability in Pancreatic Beta CellsZhen Zhang0Jing Li1Lei Yang2Rongping Chen3Rui Yang4Hua Zhang5Dehong Cai6Hong Chen7Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, ChinaDepartment of Endocrinology, Nanshan Affiliated Hospital of Guangdong Medical College, Shenzhen 518052, ChinaDepartment of Nephrology, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, ChinaDepartment of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, ChinaDepartment of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, ChinaDepartment of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, ChinaDepartment of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, ChinaDepartment of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, ChinaObjectives. Glucose fluctuations are both strong predictor of diabetic complications and crucial factor for beta cell damages. Here we investigated the effect of intermittent high glucose (IHG) on both cell apoptosis and proliferation activity in INS-1 cells and the potential mechanisms. Methods. Cells were treated with normal glucose (5.5 mmol/L), constant high glucose (CHG) (25 mmol/L), and IHG (rotation per 24 h in 11.1 or 25 mmol/L) for 7 days. Reactive oxygen species (ROS), xanthine oxidase (XOD) level, apoptosis, cell viability, cell cycle, and expression of cyclinD1, p21, p27, and Skp2 were determined. Results. We found that IHG induced more significant apoptosis than CHG and normal glucose; intracellular ROS and XOD levels were more markedly increased in cells exposed to IHG. Cells treated with IHG showed significant decreased cell viability and increased cell proportion in G0/G1 phase. Cell cycle related proteins such as cyclinD1 and Skp2 were decreased significantly, but expressions of p27 and p21 were increased markedly. Conclusions. This study suggested that IHG plays a more toxic effect including both apoptosis-inducing and antiproliferative effects on INS-1 cells. Excessive activation of cellular stress and regulation of cyclins might be potential mechanism of impairment in INS-1 cells induced by IHG.http://dx.doi.org/10.1155/2014/712781 |
| spellingShingle | Zhen Zhang Jing Li Lei Yang Rongping Chen Rui Yang Hua Zhang Dehong Cai Hong Chen The Cytotoxic Role of Intermittent High Glucose on Apoptosis and Cell Viability in Pancreatic Beta Cells Journal of Diabetes Research |
| title | The Cytotoxic Role of Intermittent High Glucose on Apoptosis and Cell Viability in Pancreatic Beta Cells |
| title_full | The Cytotoxic Role of Intermittent High Glucose on Apoptosis and Cell Viability in Pancreatic Beta Cells |
| title_fullStr | The Cytotoxic Role of Intermittent High Glucose on Apoptosis and Cell Viability in Pancreatic Beta Cells |
| title_full_unstemmed | The Cytotoxic Role of Intermittent High Glucose on Apoptosis and Cell Viability in Pancreatic Beta Cells |
| title_short | The Cytotoxic Role of Intermittent High Glucose on Apoptosis and Cell Viability in Pancreatic Beta Cells |
| title_sort | cytotoxic role of intermittent high glucose on apoptosis and cell viability in pancreatic beta cells |
| url | http://dx.doi.org/10.1155/2014/712781 |
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