PLK3 facilitates replication of swine influenza virus by phosphorylating viral NP protein
Swine H1N1/2009 influenza is a highly infectious respiratory disease in pigs, which poses a great threat to pig production and human health. In this study, we investigated the global expression profiling of swine-encoded genes in response to swine H1N1/2009 influenza A virus (SIV-H1N1/2009) in newbo...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2023.2275606 |
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| author | Caiyue Ren Tong Chen Shishuo Zhang Qingxia Gao Jiahui Zou Peng Li Biaoxiong Wang Yaxin Zhao Aotian OuYang Sizhu Suolang Hongbo Zhou |
| author_facet | Caiyue Ren Tong Chen Shishuo Zhang Qingxia Gao Jiahui Zou Peng Li Biaoxiong Wang Yaxin Zhao Aotian OuYang Sizhu Suolang Hongbo Zhou |
| author_sort | Caiyue Ren |
| collection | DOAJ |
| description | Swine H1N1/2009 influenza is a highly infectious respiratory disease in pigs, which poses a great threat to pig production and human health. In this study, we investigated the global expression profiling of swine-encoded genes in response to swine H1N1/2009 influenza A virus (SIV-H1N1/2009) in newborn pig trachea (NPTr) cells. In total, 166 genes were found to be differentially expressed (DE) according to the gene microarray. After analyzing the DE genes which might affect the SIV-H1N1/2009 replication, we focused on polo-like kinase 3 (PLK3). PLK3 is a member of the PLK family, which is a highly conserved serine/threonine kinase in eukaryotes and well known for its role in the regulation of cell cycle and cell division. We validated that the expression of PLK3 was upregulated after SIV-H1N1/2009 infection. Additionally, PLK3 was found to interact with viral nucleoprotein (NP), significantly increased NP phosphorylation and oligomerization, and promoted viral ribonucleoprotein assembly and replication. Furthermore, we identified serine 482 (S482) as the phosphorylated residue on NP by PLK3. The phosphorylation of S482 regulated NP oligomerization, viral polymerase activity and growth. Our findings provide further insights for understanding the replication of influenza A virus. |
| format | Article |
| id | doaj-art-06c0767b7e36450fa5f34caaf1c51b60 |
| institution | Kabale University |
| issn | 2222-1751 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-06c0767b7e36450fa5f34caaf1c51b602025-08-20T03:29:38ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512023-12-0112210.1080/22221751.2023.2275606PLK3 facilitates replication of swine influenza virus by phosphorylating viral NP proteinCaiyue Ren0Tong Chen1Shishuo Zhang2Qingxia Gao3Jiahui Zou4Peng Li5Biaoxiong Wang6Yaxin Zhao7Aotian OuYang8Sizhu Suolang9Hongbo Zhou10National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People’s Republic of ChinaNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People’s Republic of ChinaNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People’s Republic of ChinaNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People’s Republic of ChinaNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People’s Republic of ChinaNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People’s Republic of ChinaNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People’s Republic of ChinaNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People’s Republic of ChinaNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People’s Republic of ChinaDepartment of Animal Science, Tibet Agricultural and Animal Husbandry College, Linzhi, People’s Republic of ChinaNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People’s Republic of ChinaSwine H1N1/2009 influenza is a highly infectious respiratory disease in pigs, which poses a great threat to pig production and human health. In this study, we investigated the global expression profiling of swine-encoded genes in response to swine H1N1/2009 influenza A virus (SIV-H1N1/2009) in newborn pig trachea (NPTr) cells. In total, 166 genes were found to be differentially expressed (DE) according to the gene microarray. After analyzing the DE genes which might affect the SIV-H1N1/2009 replication, we focused on polo-like kinase 3 (PLK3). PLK3 is a member of the PLK family, which is a highly conserved serine/threonine kinase in eukaryotes and well known for its role in the regulation of cell cycle and cell division. We validated that the expression of PLK3 was upregulated after SIV-H1N1/2009 infection. Additionally, PLK3 was found to interact with viral nucleoprotein (NP), significantly increased NP phosphorylation and oligomerization, and promoted viral ribonucleoprotein assembly and replication. Furthermore, we identified serine 482 (S482) as the phosphorylated residue on NP by PLK3. The phosphorylation of S482 regulated NP oligomerization, viral polymerase activity and growth. Our findings provide further insights for understanding the replication of influenza A virus.https://www.tandfonline.com/doi/10.1080/22221751.2023.2275606Swine influenza virusPLK3nucleoproteinphosphorylationvirus replication |
| spellingShingle | Caiyue Ren Tong Chen Shishuo Zhang Qingxia Gao Jiahui Zou Peng Li Biaoxiong Wang Yaxin Zhao Aotian OuYang Sizhu Suolang Hongbo Zhou PLK3 facilitates replication of swine influenza virus by phosphorylating viral NP protein Emerging Microbes and Infections Swine influenza virus PLK3 nucleoprotein phosphorylation virus replication |
| title | PLK3 facilitates replication of swine influenza virus by phosphorylating viral NP protein |
| title_full | PLK3 facilitates replication of swine influenza virus by phosphorylating viral NP protein |
| title_fullStr | PLK3 facilitates replication of swine influenza virus by phosphorylating viral NP protein |
| title_full_unstemmed | PLK3 facilitates replication of swine influenza virus by phosphorylating viral NP protein |
| title_short | PLK3 facilitates replication of swine influenza virus by phosphorylating viral NP protein |
| title_sort | plk3 facilitates replication of swine influenza virus by phosphorylating viral np protein |
| topic | Swine influenza virus PLK3 nucleoprotein phosphorylation virus replication |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2023.2275606 |
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