Characterization of Immunogenicity of Malignant Cells with Stemness in Intrahepatic Cholangiocarcinoma by Single-Cell RNA Sequencing
Cancer stem cells (CSCs) are responsible for long-term maintenance of tumors and thought to play a role in treatment resistance. The interaction between stemness and immunogenicity of CSCs in the intrahepatic cholangiocarcinoma (iCCA) is largely unknown. Here, we used single-cell transcriptomic data...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2022/3558200 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850233869737918464 |
|---|---|
| author | Jing Bian Jianyang Fu Xin Wang Jihye Lee Gagandeep Brar Freddy E. Escorcia Maggie Cam Changqing Xie |
| author_facet | Jing Bian Jianyang Fu Xin Wang Jihye Lee Gagandeep Brar Freddy E. Escorcia Maggie Cam Changqing Xie |
| author_sort | Jing Bian |
| collection | DOAJ |
| description | Cancer stem cells (CSCs) are responsible for long-term maintenance of tumors and thought to play a role in treatment resistance. The interaction between stemness and immunogenicity of CSCs in the intrahepatic cholangiocarcinoma (iCCA) is largely unknown. Here, we used single-cell transcriptomic data to study immunogenicity of malignant cells in human iCCA. Using an established computerized method CytoTRACE, we found significant heterogeneity in stemness/differentiation states among malignant cells. We demonstrated that the high stemness malignant cells express much lower levels of major histocompatibility complex II molecules when compared to low stemness malignant cells, suggesting a role of immune evasion in high stemness malignant cells. In addition, high stemness malignant iCCA cells exhibited significant expression of certain cytokine members, including CCL2, CCL20, CXCL1, CXCL2, CXCL6, CXCL8, TNFRSF12A, and IL6ST, indicating communication with surrounding immune cells. These results indicate that high stemness malignant cells retain their intrinsic immunological feature that facilitate the escape of immune surveillance. |
| format | Article |
| id | doaj-art-06b6c77cea3047d2b9b9e4ae9cbbf8bb |
| institution | OA Journals |
| issn | 1687-9678 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-06b6c77cea3047d2b9b9e4ae9cbbf8bb2025-08-20T02:02:48ZengWileyStem Cells International1687-96782022-01-01202210.1155/2022/3558200Characterization of Immunogenicity of Malignant Cells with Stemness in Intrahepatic Cholangiocarcinoma by Single-Cell RNA SequencingJing Bian0Jianyang Fu1Xin Wang2Jihye Lee3Gagandeep Brar4Freddy E. Escorcia5Maggie Cam6Changqing Xie7CCR Collaborative Bioinformatics ResourceGastrointestinal Malignancies SectionGastrointestinal Malignancies SectionGastrointestinal Malignancies SectionSandra and Edward Meyer Cancer CenterMolecular Imaging BranchCCR Collaborative Bioinformatics ResourceGastrointestinal Malignancies SectionCancer stem cells (CSCs) are responsible for long-term maintenance of tumors and thought to play a role in treatment resistance. The interaction between stemness and immunogenicity of CSCs in the intrahepatic cholangiocarcinoma (iCCA) is largely unknown. Here, we used single-cell transcriptomic data to study immunogenicity of malignant cells in human iCCA. Using an established computerized method CytoTRACE, we found significant heterogeneity in stemness/differentiation states among malignant cells. We demonstrated that the high stemness malignant cells express much lower levels of major histocompatibility complex II molecules when compared to low stemness malignant cells, suggesting a role of immune evasion in high stemness malignant cells. In addition, high stemness malignant iCCA cells exhibited significant expression of certain cytokine members, including CCL2, CCL20, CXCL1, CXCL2, CXCL6, CXCL8, TNFRSF12A, and IL6ST, indicating communication with surrounding immune cells. These results indicate that high stemness malignant cells retain their intrinsic immunological feature that facilitate the escape of immune surveillance.http://dx.doi.org/10.1155/2022/3558200 |
| spellingShingle | Jing Bian Jianyang Fu Xin Wang Jihye Lee Gagandeep Brar Freddy E. Escorcia Maggie Cam Changqing Xie Characterization of Immunogenicity of Malignant Cells with Stemness in Intrahepatic Cholangiocarcinoma by Single-Cell RNA Sequencing Stem Cells International |
| title | Characterization of Immunogenicity of Malignant Cells with Stemness in Intrahepatic Cholangiocarcinoma by Single-Cell RNA Sequencing |
| title_full | Characterization of Immunogenicity of Malignant Cells with Stemness in Intrahepatic Cholangiocarcinoma by Single-Cell RNA Sequencing |
| title_fullStr | Characterization of Immunogenicity of Malignant Cells with Stemness in Intrahepatic Cholangiocarcinoma by Single-Cell RNA Sequencing |
| title_full_unstemmed | Characterization of Immunogenicity of Malignant Cells with Stemness in Intrahepatic Cholangiocarcinoma by Single-Cell RNA Sequencing |
| title_short | Characterization of Immunogenicity of Malignant Cells with Stemness in Intrahepatic Cholangiocarcinoma by Single-Cell RNA Sequencing |
| title_sort | characterization of immunogenicity of malignant cells with stemness in intrahepatic cholangiocarcinoma by single cell rna sequencing |
| url | http://dx.doi.org/10.1155/2022/3558200 |
| work_keys_str_mv | AT jingbian characterizationofimmunogenicityofmalignantcellswithstemnessinintrahepaticcholangiocarcinomabysinglecellrnasequencing AT jianyangfu characterizationofimmunogenicityofmalignantcellswithstemnessinintrahepaticcholangiocarcinomabysinglecellrnasequencing AT xinwang characterizationofimmunogenicityofmalignantcellswithstemnessinintrahepaticcholangiocarcinomabysinglecellrnasequencing AT jihyelee characterizationofimmunogenicityofmalignantcellswithstemnessinintrahepaticcholangiocarcinomabysinglecellrnasequencing AT gagandeepbrar characterizationofimmunogenicityofmalignantcellswithstemnessinintrahepaticcholangiocarcinomabysinglecellrnasequencing AT freddyeescorcia characterizationofimmunogenicityofmalignantcellswithstemnessinintrahepaticcholangiocarcinomabysinglecellrnasequencing AT maggiecam characterizationofimmunogenicityofmalignantcellswithstemnessinintrahepaticcholangiocarcinomabysinglecellrnasequencing AT changqingxie characterizationofimmunogenicityofmalignantcellswithstemnessinintrahepaticcholangiocarcinomabysinglecellrnasequencing |