Phosphomolybdic acid boosts polyunsaturated fatty acid and neutral lipid production in Phaeodactylum tricornutum
Phaeodactylum tricornutum is considered a potential lipid production platform due to its high growth rates and elevated natural neutral lipid and polyunsaturated fatty acid (PUFA) contents. Furthermore, microalgae are emerging as promising sources of docosahexaenoic acid (DHA) and eicosapentaenoic a...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2025.1531239/full |
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| author | Kun-Tao Peng Bing-Hong Xiong Gao Cheng Gao Cheng Yuan-Hong Zhong Yuan-Hong Zhong Lin Yu Lin Yu |
| author_facet | Kun-Tao Peng Bing-Hong Xiong Gao Cheng Gao Cheng Yuan-Hong Zhong Yuan-Hong Zhong Lin Yu Lin Yu |
| author_sort | Kun-Tao Peng |
| collection | DOAJ |
| description | Phaeodactylum tricornutum is considered a potential lipid production platform due to its high growth rates and elevated natural neutral lipid and polyunsaturated fatty acid (PUFA) contents. Furthermore, microalgae are emerging as promising sources of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). In this study, phosphomolybdic acid (PMo12), as a photocatalyst, can enhance the synthesis of neutral lipids and PUFAs by influencing the expression of lipid metabolism-related genes and photosynthesis in P. tricornutum. We also observed the contents of EPA and PUFA in engineered microalgae nearly doubled compared to the wild type, while neutral lipid content showed a significant increase of 69.7% in engineered microalgae. Notably, the growth rate of engineered microalgae remained comparable to that of the wild type. This work presents an effective approach to enhance the production of microalgal bioproducts, suggesting that photocatalysts such as PMo12 could serve as viable alternatives to genetic engineering technology, facilitating the commercialization of microalgal biodiesel. |
| format | Article |
| id | doaj-art-06b5ea2de87b46fb9d3eb4c15bc111b1 |
| institution | Kabale University |
| issn | 2296-7745 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj-art-06b5ea2de87b46fb9d3eb4c15bc111b12025-01-27T05:14:28ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452025-01-011210.3389/fmars.2025.15312391531239Phosphomolybdic acid boosts polyunsaturated fatty acid and neutral lipid production in Phaeodactylum tricornutumKun-Tao Peng0Bing-Hong Xiong1Gao Cheng2Gao Cheng3Yuan-Hong Zhong4Yuan-Hong Zhong5Lin Yu6Lin Yu7Key Laboratory of Clean Chemistry Technology of Guangdong Regular Higher Education Institutions, Guangdong Engineering Technology Research Center of Modern Fine Chemical Engineering, School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, ChinaKey Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement and Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, ChinaKey Laboratory of Clean Chemistry Technology of Guangdong Regular Higher Education Institutions, Guangdong Engineering Technology Research Center of Modern Fine Chemical Engineering, School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, ChinaJieyang Branch of Chemistry and Chemical Engineering Guangdong Laboratory (Rongjiang Laboratory), Jieyang, ChinaKey Laboratory of Clean Chemistry Technology of Guangdong Regular Higher Education Institutions, Guangdong Engineering Technology Research Center of Modern Fine Chemical Engineering, School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, ChinaJieyang Branch of Chemistry and Chemical Engineering Guangdong Laboratory (Rongjiang Laboratory), Jieyang, ChinaKey Laboratory of Clean Chemistry Technology of Guangdong Regular Higher Education Institutions, Guangdong Engineering Technology Research Center of Modern Fine Chemical Engineering, School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, ChinaJieyang Branch of Chemistry and Chemical Engineering Guangdong Laboratory (Rongjiang Laboratory), Jieyang, ChinaPhaeodactylum tricornutum is considered a potential lipid production platform due to its high growth rates and elevated natural neutral lipid and polyunsaturated fatty acid (PUFA) contents. Furthermore, microalgae are emerging as promising sources of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). In this study, phosphomolybdic acid (PMo12), as a photocatalyst, can enhance the synthesis of neutral lipids and PUFAs by influencing the expression of lipid metabolism-related genes and photosynthesis in P. tricornutum. We also observed the contents of EPA and PUFA in engineered microalgae nearly doubled compared to the wild type, while neutral lipid content showed a significant increase of 69.7% in engineered microalgae. Notably, the growth rate of engineered microalgae remained comparable to that of the wild type. This work presents an effective approach to enhance the production of microalgal bioproducts, suggesting that photocatalysts such as PMo12 could serve as viable alternatives to genetic engineering technology, facilitating the commercialization of microalgal biodiesel.https://www.frontiersin.org/articles/10.3389/fmars.2025.1531239/fullphosphomolybdic acidmicroalgaelipidpolyunsaturated fatty acidEPADHA |
| spellingShingle | Kun-Tao Peng Bing-Hong Xiong Gao Cheng Gao Cheng Yuan-Hong Zhong Yuan-Hong Zhong Lin Yu Lin Yu Phosphomolybdic acid boosts polyunsaturated fatty acid and neutral lipid production in Phaeodactylum tricornutum Frontiers in Marine Science phosphomolybdic acid microalgae lipid polyunsaturated fatty acid EPA DHA |
| title | Phosphomolybdic acid boosts polyunsaturated fatty acid and neutral lipid production in Phaeodactylum tricornutum |
| title_full | Phosphomolybdic acid boosts polyunsaturated fatty acid and neutral lipid production in Phaeodactylum tricornutum |
| title_fullStr | Phosphomolybdic acid boosts polyunsaturated fatty acid and neutral lipid production in Phaeodactylum tricornutum |
| title_full_unstemmed | Phosphomolybdic acid boosts polyunsaturated fatty acid and neutral lipid production in Phaeodactylum tricornutum |
| title_short | Phosphomolybdic acid boosts polyunsaturated fatty acid and neutral lipid production in Phaeodactylum tricornutum |
| title_sort | phosphomolybdic acid boosts polyunsaturated fatty acid and neutral lipid production in phaeodactylum tricornutum |
| topic | phosphomolybdic acid microalgae lipid polyunsaturated fatty acid EPA DHA |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2025.1531239/full |
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