A New Approach for Developing Spectrophotometric Determination of Phenylephrine Drug in Pure, Pharmaceutics and Serum Samples Using Sodium Periodate as Oxidizing Agent via a Green Method of CFIA/Merging Zone Technique
The research involved a rapid, automated and highly accurate developed CFIA/MZ technique for estimation of phenylephrine hydrochloride (PHE) in pure, dosage forms and biological sample. This method is based on oxidative coupling reaction of 2,4-dinitrophenylhydrazine (DNPH) with PHE in existen...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Baghdad, College of Science for Women
2024-01-01
|
| Series: | مجلة بغداد للعلوم |
| Subjects: | |
| Online Access: | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7671 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849247634207801344 |
|---|---|
| author | Shahad L. Hamed Bushra B. Qassim |
| author_facet | Shahad L. Hamed Bushra B. Qassim |
| author_sort | Shahad L. Hamed |
| collection | DOAJ |
| description |
The research involved a rapid, automated and highly accurate developed CFIA/MZ technique for estimation of phenylephrine hydrochloride (PHE) in pure, dosage forms and biological sample. This method is based on oxidative coupling reaction of 2,4-dinitrophenylhydrazine (DNPH) with PHE in existence of sodium periodate as oxidizing agent in alkaline medium to form a red colored product at ʎmax )520 nm (. A flow rate of 4.3 mL.min-1 using distilled water as a carrier, the method of FIA proved to be as a sensitive and economic analytical tool for estimation of PHE.
Within the concentration range of 5-300 μg.mL-1, a calibration curve was rectilinear, where the detection limit was 3.252 μg.mL-1, and the RSD% was 4.09. Recovery was 100.55% with a sample throughput of 62 sample/hr. The proposed method was successfully applied for determining PHE in serum samples and pharmaceutical formulations, the interference of excipients was studied. The mechanism of the chemical reaction has been proposed. When the suggested method's results were compared to those provided by a British Pharmacopeia reference method, there was no discernible difference between the two at the 95 percent confidence level.
|
| format | Article |
| id | doaj-art-069d5bd932c648a89863b5fa716cb6a2 |
| institution | Kabale University |
| issn | 2078-8665 2411-7986 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | University of Baghdad, College of Science for Women |
| record_format | Article |
| series | مجلة بغداد للعلوم |
| spelling | doaj-art-069d5bd932c648a89863b5fa716cb6a22025-08-20T03:58:10ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862024-01-0121110.21123/bsj.2023.7671A New Approach for Developing Spectrophotometric Determination of Phenylephrine Drug in Pure, Pharmaceutics and Serum Samples Using Sodium Periodate as Oxidizing Agent via a Green Method of CFIA/Merging Zone TechniqueShahad L. Hamed0Bushra B. Qassim1Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq. Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq. The research involved a rapid, automated and highly accurate developed CFIA/MZ technique for estimation of phenylephrine hydrochloride (PHE) in pure, dosage forms and biological sample. This method is based on oxidative coupling reaction of 2,4-dinitrophenylhydrazine (DNPH) with PHE in existence of sodium periodate as oxidizing agent in alkaline medium to form a red colored product at ʎmax )520 nm (. A flow rate of 4.3 mL.min-1 using distilled water as a carrier, the method of FIA proved to be as a sensitive and economic analytical tool for estimation of PHE. Within the concentration range of 5-300 μg.mL-1, a calibration curve was rectilinear, where the detection limit was 3.252 μg.mL-1, and the RSD% was 4.09. Recovery was 100.55% with a sample throughput of 62 sample/hr. The proposed method was successfully applied for determining PHE in serum samples and pharmaceutical formulations, the interference of excipients was studied. The mechanism of the chemical reaction has been proposed. When the suggested method's results were compared to those provided by a British Pharmacopeia reference method, there was no discernible difference between the two at the 95 percent confidence level. https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7671CFIA/MZ technique, DNPH, Pharmaceutics, Phenylephrine. HCl, Serum samples |
| spellingShingle | Shahad L. Hamed Bushra B. Qassim A New Approach for Developing Spectrophotometric Determination of Phenylephrine Drug in Pure, Pharmaceutics and Serum Samples Using Sodium Periodate as Oxidizing Agent via a Green Method of CFIA/Merging Zone Technique مجلة بغداد للعلوم CFIA/MZ technique, DNPH, Pharmaceutics, Phenylephrine. HCl, Serum samples |
| title | A New Approach for Developing Spectrophotometric Determination of Phenylephrine Drug in Pure, Pharmaceutics and Serum Samples Using Sodium Periodate as Oxidizing Agent via a Green Method of CFIA/Merging Zone Technique |
| title_full | A New Approach for Developing Spectrophotometric Determination of Phenylephrine Drug in Pure, Pharmaceutics and Serum Samples Using Sodium Periodate as Oxidizing Agent via a Green Method of CFIA/Merging Zone Technique |
| title_fullStr | A New Approach for Developing Spectrophotometric Determination of Phenylephrine Drug in Pure, Pharmaceutics and Serum Samples Using Sodium Periodate as Oxidizing Agent via a Green Method of CFIA/Merging Zone Technique |
| title_full_unstemmed | A New Approach for Developing Spectrophotometric Determination of Phenylephrine Drug in Pure, Pharmaceutics and Serum Samples Using Sodium Periodate as Oxidizing Agent via a Green Method of CFIA/Merging Zone Technique |
| title_short | A New Approach for Developing Spectrophotometric Determination of Phenylephrine Drug in Pure, Pharmaceutics and Serum Samples Using Sodium Periodate as Oxidizing Agent via a Green Method of CFIA/Merging Zone Technique |
| title_sort | new approach for developing spectrophotometric determination of phenylephrine drug in pure pharmaceutics and serum samples using sodium periodate as oxidizing agent via a green method of cfia merging zone technique |
| topic | CFIA/MZ technique, DNPH, Pharmaceutics, Phenylephrine. HCl, Serum samples |
| url | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7671 |
| work_keys_str_mv | AT shahadlhamed anewapproachfordevelopingspectrophotometricdeterminationofphenylephrinedruginpurepharmaceuticsandserumsamplesusingsodiumperiodateasoxidizingagentviaagreenmethodofcfiamergingzonetechnique AT bushrabqassim anewapproachfordevelopingspectrophotometricdeterminationofphenylephrinedruginpurepharmaceuticsandserumsamplesusingsodiumperiodateasoxidizingagentviaagreenmethodofcfiamergingzonetechnique AT shahadlhamed newapproachfordevelopingspectrophotometricdeterminationofphenylephrinedruginpurepharmaceuticsandserumsamplesusingsodiumperiodateasoxidizingagentviaagreenmethodofcfiamergingzonetechnique AT bushrabqassim newapproachfordevelopingspectrophotometricdeterminationofphenylephrinedruginpurepharmaceuticsandserumsamplesusingsodiumperiodateasoxidizingagentviaagreenmethodofcfiamergingzonetechnique |