Utilizing framework nucleic acids for integrated nano-micro interface system in circulating tumor cells (CTCs) detection, cultivation, and single-cell analysis
The detection and cultivation of circulating tumor cells (CTCs) play a crucial role in monitoring tumor recurrence, metastasis, early disease diagnosis, and assessing the effectiveness of drug treatments. This study specifically focused on investigating human breast cancer cells MCF-7 by utilizing f...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Advanced Sensor and Energy Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773045X24000426 |
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| author | Qian Chen Jie Su Xiaojun Bian Hongmin Zhang Shiqi Yang Juan Yan |
| author_facet | Qian Chen Jie Su Xiaojun Bian Hongmin Zhang Shiqi Yang Juan Yan |
| author_sort | Qian Chen |
| collection | DOAJ |
| description | The detection and cultivation of circulating tumor cells (CTCs) play a crucial role in monitoring tumor recurrence, metastasis, early disease diagnosis, and assessing the effectiveness of drug treatments. This study specifically focused on investigating human breast cancer cells MCF-7 by utilizing framework nucleic acids (FNAs) as bio-probe scaffold in conjunction with fishbone structures and three-dimensional (3D) microcavity structures within microchannels. These components collectively formed an integrated nano-micro interface system designed for a comprehensive examination of CTC detection and cell culture. The study involved the assessment and comparison of rigid 3D FNAs with distinct side lengths of 7, 13, and 26 bases. This approach not only allowed for precise regulation of the DNA biosensor interface through the manipulation of probe spacing, facilitating optimal probe-cell interactions within the microfluidic channel. Consequently, this approach significantly enhances capture efficiency and lowers the CTC detection limit to 5 cells/mL. Moreover, this research successfully observed cell proliferation and individual cell biological behavior within the 3D microcavity structure. The findings indicated that the overall cell population's proliferation was like that in static culture conditions. Although the proliferation cycle of individual cells was notably extended, cell mobility within the microcavity demonstrated their robust biological activity. These significant outcomes not only offer a practical approach for early tumor detection but also provide a valuable pathway for comprehending mechanisms of tumor development and advancement and guiding personalized treatment strategies effectively. |
| format | Article |
| id | doaj-art-0698cb1835c6433cb39b98342ac7e75c |
| institution | DOAJ |
| issn | 2773-045X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Advanced Sensor and Energy Materials |
| spelling | doaj-art-0698cb1835c6433cb39b98342ac7e75c2025-08-20T03:13:04ZengElsevierAdvanced Sensor and Energy Materials2773-045X2025-06-014210013110.1016/j.asems.2024.100131Utilizing framework nucleic acids for integrated nano-micro interface system in circulating tumor cells (CTCs) detection, cultivation, and single-cell analysisQian Chen0Jie Su1Xiaojun Bian2Hongmin Zhang3Shiqi Yang4Juan Yan5International Research Center for Food and Health, Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai Engineering Research Center of Aquatic-Product Process & Preservation, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaInternational Research Center for Food and Health, Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai Engineering Research Center of Aquatic-Product Process & Preservation, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaInternational Research Center for Food and Health, Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai Engineering Research Center of Aquatic-Product Process & Preservation, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaInternational Research Center for Food and Health, Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai Engineering Research Center of Aquatic-Product Process & Preservation, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaInternational Research Center for Food and Health, Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai Engineering Research Center of Aquatic-Product Process & Preservation, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCorresponding author.; International Research Center for Food and Health, Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai Engineering Research Center of Aquatic-Product Process & Preservation, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaThe detection and cultivation of circulating tumor cells (CTCs) play a crucial role in monitoring tumor recurrence, metastasis, early disease diagnosis, and assessing the effectiveness of drug treatments. This study specifically focused on investigating human breast cancer cells MCF-7 by utilizing framework nucleic acids (FNAs) as bio-probe scaffold in conjunction with fishbone structures and three-dimensional (3D) microcavity structures within microchannels. These components collectively formed an integrated nano-micro interface system designed for a comprehensive examination of CTC detection and cell culture. The study involved the assessment and comparison of rigid 3D FNAs with distinct side lengths of 7, 13, and 26 bases. This approach not only allowed for precise regulation of the DNA biosensor interface through the manipulation of probe spacing, facilitating optimal probe-cell interactions within the microfluidic channel. Consequently, this approach significantly enhances capture efficiency and lowers the CTC detection limit to 5 cells/mL. Moreover, this research successfully observed cell proliferation and individual cell biological behavior within the 3D microcavity structure. The findings indicated that the overall cell population's proliferation was like that in static culture conditions. Although the proliferation cycle of individual cells was notably extended, cell mobility within the microcavity demonstrated their robust biological activity. These significant outcomes not only offer a practical approach for early tumor detection but also provide a valuable pathway for comprehending mechanisms of tumor development and advancement and guiding personalized treatment strategies effectively.http://www.sciencedirect.com/science/article/pii/S2773045X24000426Tetrahedral DNA nanostructureSelf-assemblyMicrofluidic chipAptamerHuman breast cancer cellsBiosensing interface |
| spellingShingle | Qian Chen Jie Su Xiaojun Bian Hongmin Zhang Shiqi Yang Juan Yan Utilizing framework nucleic acids for integrated nano-micro interface system in circulating tumor cells (CTCs) detection, cultivation, and single-cell analysis Advanced Sensor and Energy Materials Tetrahedral DNA nanostructure Self-assembly Microfluidic chip Aptamer Human breast cancer cells Biosensing interface |
| title | Utilizing framework nucleic acids for integrated nano-micro interface system in circulating tumor cells (CTCs) detection, cultivation, and single-cell analysis |
| title_full | Utilizing framework nucleic acids for integrated nano-micro interface system in circulating tumor cells (CTCs) detection, cultivation, and single-cell analysis |
| title_fullStr | Utilizing framework nucleic acids for integrated nano-micro interface system in circulating tumor cells (CTCs) detection, cultivation, and single-cell analysis |
| title_full_unstemmed | Utilizing framework nucleic acids for integrated nano-micro interface system in circulating tumor cells (CTCs) detection, cultivation, and single-cell analysis |
| title_short | Utilizing framework nucleic acids for integrated nano-micro interface system in circulating tumor cells (CTCs) detection, cultivation, and single-cell analysis |
| title_sort | utilizing framework nucleic acids for integrated nano micro interface system in circulating tumor cells ctcs detection cultivation and single cell analysis |
| topic | Tetrahedral DNA nanostructure Self-assembly Microfluidic chip Aptamer Human breast cancer cells Biosensing interface |
| url | http://www.sciencedirect.com/science/article/pii/S2773045X24000426 |
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