Emergence of multiple drug resistance Vibrio cholerae O1 in East Delhi
Introduction: Considering the changing geographical and temporal occurrence of Vibrio cholerae, there is a continuing need to monitor the strain characteristics and antibiotic resistance patterns of this pathogen. The present study was conducted to document the changing biology of V. cholerae isola...
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The Journal of Infection in Developing Countries
2011-03-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/1251 |
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| author | Shukla Das Shilpee Choudhry Rumpa Saha Vishnampettai Ganapathysubramanian Ramachandran Kamaldeep Kaur B L Sarkar |
| author_facet | Shukla Das Shilpee Choudhry Rumpa Saha Vishnampettai Ganapathysubramanian Ramachandran Kamaldeep Kaur B L Sarkar |
| author_sort | Shukla Das |
| collection | DOAJ |
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Introduction: Considering the changing geographical and temporal occurrence of Vibrio cholerae, there is a continuing need to monitor the strain characteristics and antibiotic resistance patterns of this pathogen. The present study was conducted to document the changing biology of V. cholerae isolates in and around Delhi, India, and the development of antibiotic resistance.
Methodology: A total of 1,424 stool samples or rectal swabs from patients with acute secretory diarrhoea admitted to Guru Teg Bahadur Hospital, Delhi, between January 2007 and December 2009 were processed using standard bacteriological methods. Strains identified as V. cholerae were further subjected to serogrouping, phage typing and antimicrobial susceptibility testing. Minimum inhibitory concentration (MIC) of gentamicin and tetracycline was determined.
Results: V. cholerae was isolated in 242/1,424 (17.0%) specimens. Of these, the majority were V. cholerae O1 serotype (98.3%) and serovar Ogawa. The drugs to which V. cholerae O1 isolates showed high levels of resistance were nalidixic acid, furazolidone, and cotrimoxazole throughout the study period, whereas strains were usually susceptible to chloramphenicol and cefotaxime. In 2007, there was a sudden increase of resistance to gentamicin and tetracycline, followed by a slow reversal to previous levels in subsequent years. The phage typing pattern (Basu and Mukherjee scheme) showed a dominance of phage type 2 throughout the study period.
Conclusion: The importance of reporting all cases of V. cholerae, should be greatly emphasized, with the ultimate goal of understanding the constantly changing resistance patterns of this pathogen.
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| format | Article |
| id | doaj-art-0677eb30dd53446dab9bd32fca3ccc56 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2011-03-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-0677eb30dd53446dab9bd32fca3ccc562025-08-20T02:57:18ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802011-03-0150410.3855/jidc.1251Emergence of multiple drug resistance Vibrio cholerae O1 in East DelhiShukla Das0Shilpee Choudhry1Rumpa Saha2Vishnampettai Ganapathysubramanian Ramachandran3Kamaldeep Kaur4B L Sarkar5The University College of Medical Sciences and GTB Hospital, University of Delhi, Delhi, IndiaThe University College of Medical Sciences and GTB Hospital, University of Delhi, Delhi, IndiaThe University College of Medical Sciences and GTB Hospital, University of Delhi, Delhi, IndiaThe University College of Medical Sciences and GTB Hospital, University of Delhi, Delhi, IndiaThe University College of Medical Sciences and GTB Hospital, University of Delhi, Delhi, IndiaNational Institute of Cholera and Enteric Diseases, Beliaghata, Kolkata, India Introduction: Considering the changing geographical and temporal occurrence of Vibrio cholerae, there is a continuing need to monitor the strain characteristics and antibiotic resistance patterns of this pathogen. The present study was conducted to document the changing biology of V. cholerae isolates in and around Delhi, India, and the development of antibiotic resistance. Methodology: A total of 1,424 stool samples or rectal swabs from patients with acute secretory diarrhoea admitted to Guru Teg Bahadur Hospital, Delhi, between January 2007 and December 2009 were processed using standard bacteriological methods. Strains identified as V. cholerae were further subjected to serogrouping, phage typing and antimicrobial susceptibility testing. Minimum inhibitory concentration (MIC) of gentamicin and tetracycline was determined. Results: V. cholerae was isolated in 242/1,424 (17.0%) specimens. Of these, the majority were V. cholerae O1 serotype (98.3%) and serovar Ogawa. The drugs to which V. cholerae O1 isolates showed high levels of resistance were nalidixic acid, furazolidone, and cotrimoxazole throughout the study period, whereas strains were usually susceptible to chloramphenicol and cefotaxime. In 2007, there was a sudden increase of resistance to gentamicin and tetracycline, followed by a slow reversal to previous levels in subsequent years. The phage typing pattern (Basu and Mukherjee scheme) showed a dominance of phage type 2 throughout the study period. Conclusion: The importance of reporting all cases of V. cholerae, should be greatly emphasized, with the ultimate goal of understanding the constantly changing resistance patterns of this pathogen. https://jidc.org/index.php/journal/article/view/1251Vibrio choleraeMDRGentamicinTetracyclinephage typing |
| spellingShingle | Shukla Das Shilpee Choudhry Rumpa Saha Vishnampettai Ganapathysubramanian Ramachandran Kamaldeep Kaur B L Sarkar Emergence of multiple drug resistance Vibrio cholerae O1 in East Delhi Journal of Infection in Developing Countries Vibrio cholerae MDR Gentamicin Tetracycline phage typing |
| title | Emergence of multiple drug resistance Vibrio cholerae O1 in East Delhi |
| title_full | Emergence of multiple drug resistance Vibrio cholerae O1 in East Delhi |
| title_fullStr | Emergence of multiple drug resistance Vibrio cholerae O1 in East Delhi |
| title_full_unstemmed | Emergence of multiple drug resistance Vibrio cholerae O1 in East Delhi |
| title_short | Emergence of multiple drug resistance Vibrio cholerae O1 in East Delhi |
| title_sort | emergence of multiple drug resistance vibrio cholerae o1 in east delhi |
| topic | Vibrio cholerae MDR Gentamicin Tetracycline phage typing |
| url | https://jidc.org/index.php/journal/article/view/1251 |
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