In situ template synthesized silver nanoparticulate banana fibre materials with antimicrobial and antibiotic release properties: Efficacy evaluation in ex vivo wound infection model
This study reports the in-situ template synthesis of silver nanoparticles (SNPs) within banana fibres (BF), non-woven sheets (BFS), and microparticles (BFM), yielding multifunctional biocomposites with broad-spectrum antimicrobial properties and controlled antibiotic release capabilities. The prepar...
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Elsevier
2025-01-01
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| Series: | Next Nanotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2949829525000038 |
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| author | Pompi Das Debajit Mahanta Sharmila Giogi Tarh Kaha Ngurang Nisha Sanjeeb Kalita |
| author_facet | Pompi Das Debajit Mahanta Sharmila Giogi Tarh Kaha Ngurang Nisha Sanjeeb Kalita |
| author_sort | Pompi Das |
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| description | This study reports the in-situ template synthesis of silver nanoparticles (SNPs) within banana fibres (BF), non-woven sheets (BFS), and microparticles (BFM), yielding multifunctional biocomposites with broad-spectrum antimicrobial properties and controlled antibiotic release capabilities. The prepared SNPs exhibited a uniform size distribution with an average diameter of 12.6 ± 2.4 nm, confirmed through field emission scanning electron microscopy (FESEM) and energy-dispersive X-ray spectroscopy (EDX). Attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) analysis indicated strong interactions between the banana fibre matrix and SNPs, with characteristic peaks at 1384 and 1612 cm⁻¹ corresponding to the Ag-O bonds. Thermogravimetric analysis (TGA) revealed enhanced thermal stability, with BFS-SNP showing a 25 % improvement in decomposition onset temperature compared to pristine BFS. Mechanical testing demonstrated improved tensile strength in SNP-modified sheets (21.5 ± 0.8 MPa) compared to untreated sheets (16.8 ± 0.7 MPa), highlighting the reinforcement effect of SNP integration. The biocomposites exhibited potent antibacterial activity against Escherichia coli, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus (MRSA), with inhibition zones ranging from 18.5 ± 1.2 mm to 22.3 ± 1.1 mm. Controlled release studies of amoxicillin-loaded composites demonstrated sustained drug release over 72 hours, achieving a cumulative release of 81.6 % in BFS-SNP-AMOX. Cytotoxicity assessment on L929 fibroblasts confirmed the biocompatibility of the composites, with cell viabilities exceeding 90 %. These findings establish BF-SNP, BFS-SNP, and BFM-SNP as promising candidates for antimicrobial wound care applications and controlled drug delivery systems, offering a sustainable, bioresource-based solution for advanced biomedical materials. |
| format | Article |
| id | doaj-art-06707abb08b54707a13c9947e3af0a25 |
| institution | Kabale University |
| issn | 2949-8295 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Next Nanotechnology |
| spelling | doaj-art-06707abb08b54707a13c9947e3af0a252025-02-11T04:35:45ZengElsevierNext Nanotechnology2949-82952025-01-017100134In situ template synthesized silver nanoparticulate banana fibre materials with antimicrobial and antibiotic release properties: Efficacy evaluation in ex vivo wound infection modelPompi Das0Debajit Mahanta1Sharmila Giogi2Tarh Kaha3Ngurang Nisha4Sanjeeb Kalita5Microbial Biotechnology Laboratory, DBT-APSCS&T CoE for Bioresources and Sustainable Development, Kimin, Arunachal Pradesh, IndiaMicrobial Biotechnology Laboratory, DBT-APSCS&T CoE for Bioresources and Sustainable Development, Kimin, Arunachal Pradesh, India; Translational Biotechnology Research Laboratory, DBT-APSCS&T CoE for Bioresources and Sustainable Development, Kimin, Arunachal Pradesh, IndiaMicrobial Biotechnology Laboratory, DBT-APSCS&T CoE for Bioresources and Sustainable Development, Kimin, Arunachal Pradesh, IndiaMicrobial Biotechnology Laboratory, DBT-APSCS&T CoE for Bioresources and Sustainable Development, Kimin, Arunachal Pradesh, IndiaTranslational Biotechnology Research Laboratory, DBT-APSCS&T CoE for Bioresources and Sustainable Development, Kimin, Arunachal Pradesh, IndiaTranslational Biotechnology Research Laboratory, DBT-APSCS&T CoE for Bioresources and Sustainable Development, Kimin, Arunachal Pradesh, India; Corresponding author.This study reports the in-situ template synthesis of silver nanoparticles (SNPs) within banana fibres (BF), non-woven sheets (BFS), and microparticles (BFM), yielding multifunctional biocomposites with broad-spectrum antimicrobial properties and controlled antibiotic release capabilities. The prepared SNPs exhibited a uniform size distribution with an average diameter of 12.6 ± 2.4 nm, confirmed through field emission scanning electron microscopy (FESEM) and energy-dispersive X-ray spectroscopy (EDX). Attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) analysis indicated strong interactions between the banana fibre matrix and SNPs, with characteristic peaks at 1384 and 1612 cm⁻¹ corresponding to the Ag-O bonds. Thermogravimetric analysis (TGA) revealed enhanced thermal stability, with BFS-SNP showing a 25 % improvement in decomposition onset temperature compared to pristine BFS. Mechanical testing demonstrated improved tensile strength in SNP-modified sheets (21.5 ± 0.8 MPa) compared to untreated sheets (16.8 ± 0.7 MPa), highlighting the reinforcement effect of SNP integration. The biocomposites exhibited potent antibacterial activity against Escherichia coli, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus (MRSA), with inhibition zones ranging from 18.5 ± 1.2 mm to 22.3 ± 1.1 mm. Controlled release studies of amoxicillin-loaded composites demonstrated sustained drug release over 72 hours, achieving a cumulative release of 81.6 % in BFS-SNP-AMOX. Cytotoxicity assessment on L929 fibroblasts confirmed the biocompatibility of the composites, with cell viabilities exceeding 90 %. These findings establish BF-SNP, BFS-SNP, and BFM-SNP as promising candidates for antimicrobial wound care applications and controlled drug delivery systems, offering a sustainable, bioresource-based solution for advanced biomedical materials.http://www.sciencedirect.com/science/article/pii/S2949829525000038Banana fibreIn-situ template synthesisSNPsBroad spectrum antimicrobial activityMRSABiocompatible |
| spellingShingle | Pompi Das Debajit Mahanta Sharmila Giogi Tarh Kaha Ngurang Nisha Sanjeeb Kalita In situ template synthesized silver nanoparticulate banana fibre materials with antimicrobial and antibiotic release properties: Efficacy evaluation in ex vivo wound infection model Next Nanotechnology Banana fibre In-situ template synthesis SNPs Broad spectrum antimicrobial activity MRSA Biocompatible |
| title | In situ template synthesized silver nanoparticulate banana fibre materials with antimicrobial and antibiotic release properties: Efficacy evaluation in ex vivo wound infection model |
| title_full | In situ template synthesized silver nanoparticulate banana fibre materials with antimicrobial and antibiotic release properties: Efficacy evaluation in ex vivo wound infection model |
| title_fullStr | In situ template synthesized silver nanoparticulate banana fibre materials with antimicrobial and antibiotic release properties: Efficacy evaluation in ex vivo wound infection model |
| title_full_unstemmed | In situ template synthesized silver nanoparticulate banana fibre materials with antimicrobial and antibiotic release properties: Efficacy evaluation in ex vivo wound infection model |
| title_short | In situ template synthesized silver nanoparticulate banana fibre materials with antimicrobial and antibiotic release properties: Efficacy evaluation in ex vivo wound infection model |
| title_sort | in situ template synthesized silver nanoparticulate banana fibre materials with antimicrobial and antibiotic release properties efficacy evaluation in ex vivo wound infection model |
| topic | Banana fibre In-situ template synthesis SNPs Broad spectrum antimicrobial activity MRSA Biocompatible |
| url | http://www.sciencedirect.com/science/article/pii/S2949829525000038 |
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