Comparison of qPCR protocols for quantification of "Candidatus Saccharibacteria", belonging to the Candidate Phyla Radiation, suggests that 23S rRNA is a better target than 16S rRNA.

Comparison of qPCR protocols for quantification of "Candidatus Saccharibacteria", belonging to the Candidate Phyla Radiation, suggests that 23S rRNA is a better target than 16S rRNA.

<h4>Background</h4>Candidate Phyla Radiation (CPR) is a large monophyletic group encompassing about 25% of bacterial diversity. Among CPR, "Candidatus Saccharibacteria" is one of the most clinically relevant phyla. Indeed, it is enriched in the oral microbiota of subjects suffe...

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Main Authors: Stella Papaleo, Riccardo Nodari, Lodovico Sterzi, Enza D'Auria, Camilla Cattaneo, Giorgia Bettoni, Clara Bonaiti, Ella Pagliarini, Gianvincenzo Zuccotti, Simona Panelli, Francesco Comandatore
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2024-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0310675
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