Influence of spore morphology on spectrophotometric quantification of Trichoderma inocula
Species of the genus Trichoderma are filamentous fungi commonly used in research, industry and agriculture. Trichoderma reesei strains are prominent producers of cellulolytic and hemicellulolytic enzymes as well as being expression hosts; several other species such as T. atroviride might be exploite...
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Taylor & Francis Group
2020-05-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/btn-2019-0152 |
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| author | Georg Schütz Dietmar Haltrich Lea Atanasova |
| author_facet | Georg Schütz Dietmar Haltrich Lea Atanasova |
| author_sort | Georg Schütz |
| collection | DOAJ |
| description | Species of the genus Trichoderma are filamentous fungi commonly used in research, industry and agriculture. Trichoderma reesei strains are prominent producers of cellulolytic and hemicellulolytic enzymes as well as being expression hosts; several other species such as T. atroviride might be exploited as biocontrol agents. A careful preparation of Trichoderma inocula, which consists mainly of conidia (asexual spores), is of immense importance. Conidia concentration is still mostly determined with the help of a hemocytometer; however, as a more accurate and time-saving alternative, absorbance can be used to estimate fungal spore counts. We established a spectrophotometric method for fast and reliable preparation of Trichoderma inocula by evaluating the effect of size, shape and pigmentation of the conidia at different wavelengths. |
| format | Article |
| id | doaj-art-06445a6d91aa41f3b64124e14a2422d3 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2020-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-06445a6d91aa41f3b64124e14a2422d32025-08-20T02:26:09ZengTaylor & Francis GroupBioTechniques0736-62051940-98182020-05-0168527928210.2144/btn-2019-0152Influence of spore morphology on spectrophotometric quantification of Trichoderma inoculaGeorg Schütz0Dietmar Haltrich1Lea Atanasova21Department of Food Sciences & Technology, BOKU-University of Natural Resources & Life Sciences, Vienna, Austria1Department of Food Sciences & Technology, BOKU-University of Natural Resources & Life Sciences, Vienna, Austria1Department of Food Sciences & Technology, BOKU-University of Natural Resources & Life Sciences, Vienna, AustriaSpecies of the genus Trichoderma are filamentous fungi commonly used in research, industry and agriculture. Trichoderma reesei strains are prominent producers of cellulolytic and hemicellulolytic enzymes as well as being expression hosts; several other species such as T. atroviride might be exploited as biocontrol agents. A careful preparation of Trichoderma inocula, which consists mainly of conidia (asexual spores), is of immense importance. Conidia concentration is still mostly determined with the help of a hemocytometer; however, as a more accurate and time-saving alternative, absorbance can be used to estimate fungal spore counts. We established a spectrophotometric method for fast and reliable preparation of Trichoderma inocula by evaluating the effect of size, shape and pigmentation of the conidia at different wavelengths.https://www.future-science.com/doi/10.2144/btn-2019-0152conidiahemocytometerspectrophotometerspore concentrationspore suspensionTrichoderma atroviride |
| spellingShingle | Georg Schütz Dietmar Haltrich Lea Atanasova Influence of spore morphology on spectrophotometric quantification of Trichoderma inocula BioTechniques conidia hemocytometer spectrophotometer spore concentration spore suspension Trichoderma atroviride |
| title | Influence of spore morphology on spectrophotometric quantification of Trichoderma inocula |
| title_full | Influence of spore morphology on spectrophotometric quantification of Trichoderma inocula |
| title_fullStr | Influence of spore morphology on spectrophotometric quantification of Trichoderma inocula |
| title_full_unstemmed | Influence of spore morphology on spectrophotometric quantification of Trichoderma inocula |
| title_short | Influence of spore morphology on spectrophotometric quantification of Trichoderma inocula |
| title_sort | influence of spore morphology on spectrophotometric quantification of trichoderma inocula |
| topic | conidia hemocytometer spectrophotometer spore concentration spore suspension Trichoderma atroviride |
| url | https://www.future-science.com/doi/10.2144/btn-2019-0152 |
| work_keys_str_mv | AT georgschutz influenceofsporemorphologyonspectrophotometricquantificationoftrichodermainocula AT dietmarhaltrich influenceofsporemorphologyonspectrophotometricquantificationoftrichodermainocula AT leaatanasova influenceofsporemorphologyonspectrophotometricquantificationoftrichodermainocula |