Effects of multidrug-resistant bacteria and multi-antibiotic combination on intestinal microbiota in mice
Multidrug-resistant bacteria are a clinical and an epidemiological challenge. However, the effects of multidrug-resistant bacteria and multi-antibiotic combination on gut microbiota are unclear. In this study, the effects of multidrug-resistant bacteria and multi-antibiotic combination on intestinal...
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Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2025-02-01
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Series: | Frontiers in Microbiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1504396/full |
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Summary: | Multidrug-resistant bacteria are a clinical and an epidemiological challenge. However, the effects of multidrug-resistant bacteria and multi-antibiotic combination on gut microbiota are unclear. In this study, the effects of multidrug-resistant bacteria and multi-antibiotic combination on intestinal microbiota in mice have been observed by high-throughput sequencing. Resistant Escherichia coli (RP4) and 0.5, 1, and 2 mg/L antibiotics (Amp, Km, and Tet multi-antibiotic combination) could decrease the number of specific operational taxonomic units from 223 in the normal saline control group to 178 in the antibiotic-resistant bacteria group and 34 in the antibiotic group, and antibiotics are the biggest influencing factor. Multidrug-resistant bacteria and multi-antibiotic combination could affect the function of intestinal microbiota, and the effect of multidrug-resistant bacteria was similar to that of multi-antibiotic combination. Small intestine is the main colonization site of antibiotic-resistant bacteria, and Proteobacteria and Bacteroidetes are the major antibiotic-resistance acquired bacteria as determined by transmission electron microscopy and agarose plate screening culture. |
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ISSN: | 1664-302X |