Overexpression of miR-21-5p as a potential pathogenesis marker for ovarian endometriosis
Abstract Background Endometriosis is a benign gynecological disease characterized by the growth of endometrial cells beyond the uterus, forming endometriotic cyst tissues called ovarian endometriomas. MicroRNAs (miRNAs) are small, non-coding RNAs that epigenetically control the physiological and pat...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2025-01-01
|
| Series: | Journal of Basic and Applied Zoology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s41936-024-00414-5 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559188582432768 |
|---|---|
| author | Mohamed Mahmoud Amr Ahmed WalyEldeen Mohamed A. Samie Ahmed M. Sadek Sayed Elakhras Mohamed Samir Osama Ahmed Rasha Mohamed Samir Sayed Ahmed Abdelaziz Baiomy Sherif Abdelaziz Ibrahim Hebatallah Hassan |
| author_facet | Mohamed Mahmoud Amr Ahmed WalyEldeen Mohamed A. Samie Ahmed M. Sadek Sayed Elakhras Mohamed Samir Osama Ahmed Rasha Mohamed Samir Sayed Ahmed Abdelaziz Baiomy Sherif Abdelaziz Ibrahim Hebatallah Hassan |
| author_sort | Mohamed Mahmoud |
| collection | DOAJ |
| description | Abstract Background Endometriosis is a benign gynecological disease characterized by the growth of endometrial cells beyond the uterus, forming endometriotic cyst tissues called ovarian endometriomas. MicroRNAs (miRNAs) are small, non-coding RNAs that epigenetically control the physiological and pathological processes of different diseases, including endometriosis. In this study, we screened the expression levels of 11-selected miRNAs, namely miR-21-5p, miR-200c-3p, miR-19a-3p, miR-203-3p, miR-181b-5p, miR-182-5p, miR-let7a-5p, miR-205-5p, miR-200b-3p, miR-16-5p, and miR-222-3p in ovarian endometriomas relative to eutopic endometrial tissues using quantitative real-time PCR (qPCR). In addition, the level of mRNA expression of lumican (LUM), an extracellular matrix proteoglycan (PG), and a putative target of miR-21-5p was quantified by qPCR. Results Our screening qPCR results showed that 9 miRNAs were upregulated (miR-21-5p, miR-200c-3p, miR-19a-3p, miR-203-3p, miR-181b-5p, miR-182-5p, miR-let7a-5p, miR-205-5p, and miR-200b-3p), whereas 2 miRNAs were downregulated (miR-16-5p and miR-222-3p) in ovarian endometriomas compared to eutopic endometrium. A significant overexpression of miR-21-5p in ovarian endometrioma was further independently verified by qPCR. Using bioinformatics tools, Gene Ontology Kyoto Encyclopedia of Genes and Genomes pathways, and protein–protein interactions, we identified differentially expressed genes and several pathways regulated by miR-21-5p that may contribute to endometriosis progression. Among them, LUM was found to be significantly diminished expressed in ovarian endometriomas compared to eutopic endometrium. Conclusion In conclusion, this study identified miR-21-5p and LUM as potential factors that may contribute to ovarian endometriomas’ pathogenesis. |
| format | Article |
| id | doaj-art-063548a5efa84c709a894e0190b89302 |
| institution | Kabale University |
| issn | 2090-990X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Basic and Applied Zoology |
| spelling | doaj-art-063548a5efa84c709a894e0190b893022025-01-05T12:42:22ZengSpringerOpenJournal of Basic and Applied Zoology2090-990X2025-01-0186111510.1186/s41936-024-00414-5Overexpression of miR-21-5p as a potential pathogenesis marker for ovarian endometriosisMohamed Mahmoud0Amr Ahmed WalyEldeen1Mohamed A. Samie2Ahmed M. Sadek3Sayed Elakhras4Mohamed Samir5Osama Ahmed6Rasha Mohamed Samir Sayed7Ahmed Abdelaziz Baiomy8Sherif Abdelaziz Ibrahim9Hebatallah Hassan10Department of Zoology, Faculty of Science, Cairo UniversityDepartment of Zoology, Faculty of Science, Cairo UniversityDepartment of Gynecology and Obstetrics, Faculty of Medicine, Al-Azhar UniversityDepartment of Gynecology and Obstetrics, Faculty of Medicine, Benha UniversityConsultant of Obstetrics and Gynecology and Minimally Invasive Surgery, Omam Comprehensive Endometriosis CentreDepartment of Zoology, Faculty of Science, Cairo UniversityDepartment of Zoology, Faculty of Science, Cairo UniversityPathology Department, Faculty of Medicine, Aswan UniversityDepartment of Zoology, Faculty of Science, Cairo UniversityDepartment of Zoology, Faculty of Science, Cairo UniversityDepartment of Zoology, Faculty of Science, Cairo UniversityAbstract Background Endometriosis is a benign gynecological disease characterized by the growth of endometrial cells beyond the uterus, forming endometriotic cyst tissues called ovarian endometriomas. MicroRNAs (miRNAs) are small, non-coding RNAs that epigenetically control the physiological and pathological processes of different diseases, including endometriosis. In this study, we screened the expression levels of 11-selected miRNAs, namely miR-21-5p, miR-200c-3p, miR-19a-3p, miR-203-3p, miR-181b-5p, miR-182-5p, miR-let7a-5p, miR-205-5p, miR-200b-3p, miR-16-5p, and miR-222-3p in ovarian endometriomas relative to eutopic endometrial tissues using quantitative real-time PCR (qPCR). In addition, the level of mRNA expression of lumican (LUM), an extracellular matrix proteoglycan (PG), and a putative target of miR-21-5p was quantified by qPCR. Results Our screening qPCR results showed that 9 miRNAs were upregulated (miR-21-5p, miR-200c-3p, miR-19a-3p, miR-203-3p, miR-181b-5p, miR-182-5p, miR-let7a-5p, miR-205-5p, and miR-200b-3p), whereas 2 miRNAs were downregulated (miR-16-5p and miR-222-3p) in ovarian endometriomas compared to eutopic endometrium. A significant overexpression of miR-21-5p in ovarian endometrioma was further independently verified by qPCR. Using bioinformatics tools, Gene Ontology Kyoto Encyclopedia of Genes and Genomes pathways, and protein–protein interactions, we identified differentially expressed genes and several pathways regulated by miR-21-5p that may contribute to endometriosis progression. Among them, LUM was found to be significantly diminished expressed in ovarian endometriomas compared to eutopic endometrium. Conclusion In conclusion, this study identified miR-21-5p and LUM as potential factors that may contribute to ovarian endometriomas’ pathogenesis.https://doi.org/10.1186/s41936-024-00414-5EndometriosisOvarian endometriomasmicroRNAmiR-21Lumican |
| spellingShingle | Mohamed Mahmoud Amr Ahmed WalyEldeen Mohamed A. Samie Ahmed M. Sadek Sayed Elakhras Mohamed Samir Osama Ahmed Rasha Mohamed Samir Sayed Ahmed Abdelaziz Baiomy Sherif Abdelaziz Ibrahim Hebatallah Hassan Overexpression of miR-21-5p as a potential pathogenesis marker for ovarian endometriosis Journal of Basic and Applied Zoology Endometriosis Ovarian endometriomas microRNA miR-21 Lumican |
| title | Overexpression of miR-21-5p as a potential pathogenesis marker for ovarian endometriosis |
| title_full | Overexpression of miR-21-5p as a potential pathogenesis marker for ovarian endometriosis |
| title_fullStr | Overexpression of miR-21-5p as a potential pathogenesis marker for ovarian endometriosis |
| title_full_unstemmed | Overexpression of miR-21-5p as a potential pathogenesis marker for ovarian endometriosis |
| title_short | Overexpression of miR-21-5p as a potential pathogenesis marker for ovarian endometriosis |
| title_sort | overexpression of mir 21 5p as a potential pathogenesis marker for ovarian endometriosis |
| topic | Endometriosis Ovarian endometriomas microRNA miR-21 Lumican |
| url | https://doi.org/10.1186/s41936-024-00414-5 |
| work_keys_str_mv | AT mohamedmahmoud overexpressionofmir215pasapotentialpathogenesismarkerforovarianendometriosis AT amrahmedwalyeldeen overexpressionofmir215pasapotentialpathogenesismarkerforovarianendometriosis AT mohamedasamie overexpressionofmir215pasapotentialpathogenesismarkerforovarianendometriosis AT ahmedmsadek overexpressionofmir215pasapotentialpathogenesismarkerforovarianendometriosis AT sayedelakhras overexpressionofmir215pasapotentialpathogenesismarkerforovarianendometriosis AT mohamedsamir overexpressionofmir215pasapotentialpathogenesismarkerforovarianendometriosis AT osamaahmed overexpressionofmir215pasapotentialpathogenesismarkerforovarianendometriosis AT rashamohamedsamirsayed overexpressionofmir215pasapotentialpathogenesismarkerforovarianendometriosis AT ahmedabdelazizbaiomy overexpressionofmir215pasapotentialpathogenesismarkerforovarianendometriosis AT sherifabdelazizibrahim overexpressionofmir215pasapotentialpathogenesismarkerforovarianendometriosis AT hebatallahhassan overexpressionofmir215pasapotentialpathogenesismarkerforovarianendometriosis |