A putative gene-for-gene relationship between the Erwinia amylovora effector gene eop1 and the FB_Mar12 resistance locus of Malus ×arnoldiana accession MAL0004
The bacterial pathogen Erwinia amylovora causes fire blight on rosaceous plants, including apples and their wild relatives. The pathogen uses the type III secretion pathogenicity island to inject effector proteins, such as Eop1, into host plants, leading to disease phenotypes in susceptible genotype...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1472536/full |
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| author | Ofere Francis Emeriewen Holger Zetzsche Thomas Wolfgang Wöhner Henryk Flachowsky Andreas Peil |
| author_facet | Ofere Francis Emeriewen Holger Zetzsche Thomas Wolfgang Wöhner Henryk Flachowsky Andreas Peil |
| author_sort | Ofere Francis Emeriewen |
| collection | DOAJ |
| description | The bacterial pathogen Erwinia amylovora causes fire blight on rosaceous plants,
including apples and their wild relatives. The pathogen uses the type III secretion pathogenicity island to inject effector proteins, such as Eop1, into host plants, leading to disease phenotypes in susceptible genotypes. In contrast, resistant genotypes exhibit quantitative resistance associated with genomic regions and/or R-gene-mediated qualitative resistance to withstand the pathogen. In Malus, strong resistance is observed in some wild species accessions, for example, in Malus xarnoldiana accession MAL0004. The resistance locus FB_Mar12, previously identified on linkage group 12 (LG12) of MAL0004, is one of two gene loci in Malus proven to withstand highly virulent North American strains of E. amylovora. This suggests the influence of a major gene, with a few candidate genes proposed within the FB_Mar12 region. In this report, we provide evidence that this gene locus is completely broken down by a mutant strain of the E. amylovora effector protein Eop1 (Δeop1) following artificial shoot inoculations of an ‘Idared’ × MAL0004 F1 progeny set, indicating a gene-for-gene interaction. Interestingly, Δeop1 does not overcome the resistance of the FB_Mar12 donor MAL0004 itself, but only the QTL on LG12, an indication that other resistance factors, possibly QTLs/genes are contributing to the fire blight resistance of MAL0004. |
| format | Article |
| id | doaj-art-06243b178f584c42899b87f7d7d83bf8 |
| institution | OA Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-06243b178f584c42899b87f7d7d83bf82025-08-20T02:30:47ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-12-011510.3389/fpls.2024.14725361472536A putative gene-for-gene relationship between the Erwinia amylovora effector gene eop1 and the FB_Mar12 resistance locus of Malus ×arnoldiana accession MAL0004Ofere Francis Emeriewen0Holger Zetzsche1Thomas Wolfgang Wöhner2Henryk Flachowsky3Andreas Peil4Julius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, Dresden, GermanyJulius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Quedlinburg, GermanyJulius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, Dresden, GermanyJulius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, Dresden, GermanyJulius Kühn-Institut (JKI), Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, Dresden, GermanyThe bacterial pathogen Erwinia amylovora causes fire blight on rosaceous plants, including apples and their wild relatives. The pathogen uses the type III secretion pathogenicity island to inject effector proteins, such as Eop1, into host plants, leading to disease phenotypes in susceptible genotypes. In contrast, resistant genotypes exhibit quantitative resistance associated with genomic regions and/or R-gene-mediated qualitative resistance to withstand the pathogen. In Malus, strong resistance is observed in some wild species accessions, for example, in Malus xarnoldiana accession MAL0004. The resistance locus FB_Mar12, previously identified on linkage group 12 (LG12) of MAL0004, is one of two gene loci in Malus proven to withstand highly virulent North American strains of E. amylovora. This suggests the influence of a major gene, with a few candidate genes proposed within the FB_Mar12 region. In this report, we provide evidence that this gene locus is completely broken down by a mutant strain of the E. amylovora effector protein Eop1 (Δeop1) following artificial shoot inoculations of an ‘Idared’ × MAL0004 F1 progeny set, indicating a gene-for-gene interaction. Interestingly, Δeop1 does not overcome the resistance of the FB_Mar12 donor MAL0004 itself, but only the QTL on LG12, an indication that other resistance factors, possibly QTLs/genes are contributing to the fire blight resistance of MAL0004.https://www.frontiersin.org/articles/10.3389/fpls.2024.1472536/fullapple wild speciesfire blightresistance QTLresistance-breakdownMalus hosts |
| spellingShingle | Ofere Francis Emeriewen Holger Zetzsche Thomas Wolfgang Wöhner Henryk Flachowsky Andreas Peil A putative gene-for-gene relationship between the Erwinia amylovora effector gene eop1 and the FB_Mar12 resistance locus of Malus ×arnoldiana accession MAL0004 Frontiers in Plant Science apple wild species fire blight resistance QTL resistance-breakdown Malus hosts |
| title | A putative gene-for-gene relationship between the Erwinia amylovora effector gene eop1 and the FB_Mar12 resistance locus of Malus ×arnoldiana accession MAL0004 |
| title_full | A putative gene-for-gene relationship between the Erwinia amylovora effector gene eop1 and the FB_Mar12 resistance locus of Malus ×arnoldiana accession MAL0004 |
| title_fullStr | A putative gene-for-gene relationship between the Erwinia amylovora effector gene eop1 and the FB_Mar12 resistance locus of Malus ×arnoldiana accession MAL0004 |
| title_full_unstemmed | A putative gene-for-gene relationship between the Erwinia amylovora effector gene eop1 and the FB_Mar12 resistance locus of Malus ×arnoldiana accession MAL0004 |
| title_short | A putative gene-for-gene relationship between the Erwinia amylovora effector gene eop1 and the FB_Mar12 resistance locus of Malus ×arnoldiana accession MAL0004 |
| title_sort | putative gene for gene relationship between the erwinia amylovora effector gene eop1 and the fb mar12 resistance locus of malus arnoldiana accession mal0004 |
| topic | apple wild species fire blight resistance QTL resistance-breakdown Malus hosts |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1472536/full |
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