Regulation of the non-selective Na+ importer capacity of NRT1.2/NPF4.6/AIT1 by SOS2-mediated phosphorylation in Arabidopsis
Summary: Although preventing sodium ion import is crucial for salt tolerance, the mechanisms and the Na+ importers involved remain unclear. We identified NRT1.2/NPF4.6/AIT1 as a non-selective Na+ importer. NRT1.2 overexpression in Arabidopsis increased salt sensitivity due to Na+ accumulation, while...
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Elsevier
2025-06-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124725005005 |
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| author | Xiaoxiao Liu Lei Zhang Zeqi Zhao Yue Zheng Yue Ren Xiaomin Zhao Shizhong Zhang Guodong Yang Jinguang Huang Kang Yan Chunlong Li Chengchao Zheng Changai Wu |
| author_facet | Xiaoxiao Liu Lei Zhang Zeqi Zhao Yue Zheng Yue Ren Xiaomin Zhao Shizhong Zhang Guodong Yang Jinguang Huang Kang Yan Chunlong Li Chengchao Zheng Changai Wu |
| author_sort | Xiaoxiao Liu |
| collection | DOAJ |
| description | Summary: Although preventing sodium ion import is crucial for salt tolerance, the mechanisms and the Na+ importers involved remain unclear. We identified NRT1.2/NPF4.6/AIT1 as a non-selective Na+ importer. NRT1.2 overexpression in Arabidopsis increased salt sensitivity due to Na+ accumulation, while nrt1.2 mutants exhibited opposite results. Non-invasive micro-test technology indicated Na+ uptake capability of NRT1.2 in Arabidopsis roots. When expressed in Xenopus laevis oocytes, NRT1.2 displayed low-pH-dependent and NO3−-promoted Na+, K+, and Li+ importer capacity, conferring higher potassium and lithium tolerance in NRT1.2 overexpression plants. Mechanically, salt overly sensitive 2 (SOS2) phosphorylated NRT1.2 at Thr248, reducing its Na+ import and preventing excessive Na+ accumulation. Prolonged salt stress also downregulated NRT1.2 transcripts. In summary, our findings unveil a role of NRT1.2 in Na+ transport and a regulatory pathway via SOS2-mediated phosphorylation of NRT1.2 at Thr248, crucial for plant salt-stress adaptation. |
| format | Article |
| id | doaj-art-06194d3d9dbd4f4f959b0e8541baec46 |
| institution | DOAJ |
| issn | 2211-1247 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj-art-06194d3d9dbd4f4f959b0e8541baec462025-08-20T03:13:03ZengElsevierCell Reports2211-12472025-06-0144611572910.1016/j.celrep.2025.115729Regulation of the non-selective Na+ importer capacity of NRT1.2/NPF4.6/AIT1 by SOS2-mediated phosphorylation in ArabidopsisXiaoxiao Liu0Lei Zhang1Zeqi Zhao2Yue Zheng3Yue Ren4Xiaomin Zhao5Shizhong Zhang6Guodong Yang7Jinguang Huang8Kang Yan9Chunlong Li10Chengchao Zheng11Changai Wu12State Key Laboratory of Wheat Improvement, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Engineering Center of Saline-Alkali Soil Plant-Microbial Joint Restoration, College of Life Sciences, Shandong Agricultural University, Shandong, Tai’an 271018, ChinaState Key Laboratory of Wheat Improvement, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Engineering Center of Saline-Alkali Soil Plant-Microbial Joint Restoration, College of Life Sciences, Shandong Agricultural University, Shandong, Tai’an 271018, China; Research Institute for Marine Traditional Chinese Medicine (Qingdao Academy of Chinese Medical Sciences), The SATCM Key Unit of Discovering and Developing New Marine TCM Drugs, Key Laboratory of Marine Traditional Chinese Medicine in Shandong Universities, Shandong University of Traditional Chinese Medicine, Jinan 250355, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Hubei Hongshan Laboratory, Wuhan 430070, ChinaState Key Laboratory of Wheat Improvement, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Engineering Center of Saline-Alkali Soil Plant-Microbial Joint Restoration, College of Life Sciences, Shandong Agricultural University, Shandong, Tai’an 271018, ChinaState Key Laboratory of Wheat Improvement, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Engineering Center of Saline-Alkali Soil Plant-Microbial Joint Restoration, College of Life Sciences, Shandong Agricultural University, Shandong, Tai’an 271018, ChinaState Key Laboratory of Wheat Improvement, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Engineering Center of Saline-Alkali Soil Plant-Microbial Joint Restoration, College of Life Sciences, Shandong Agricultural University, Shandong, Tai’an 271018, ChinaState Key Laboratory of Wheat Improvement, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Engineering Center of Saline-Alkali Soil Plant-Microbial Joint Restoration, College of Life Sciences, Shandong Agricultural University, Shandong, Tai’an 271018, ChinaState Key Laboratory of Wheat Improvement, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Engineering Center of Saline-Alkali Soil Plant-Microbial Joint Restoration, College of Life Sciences, Shandong Agricultural University, Shandong, Tai’an 271018, ChinaState Key Laboratory of Wheat Improvement, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Engineering Center of Saline-Alkali Soil Plant-Microbial Joint Restoration, College of Life Sciences, Shandong Agricultural University, Shandong, Tai’an 271018, ChinaState Key Laboratory of Wheat Improvement, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Engineering Center of Saline-Alkali Soil Plant-Microbial Joint Restoration, College of Life Sciences, Shandong Agricultural University, Shandong, Tai’an 271018, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Hubei Hongshan Laboratory, Wuhan 430070, China; Corresponding authorState Key Laboratory of Wheat Improvement, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Engineering Center of Saline-Alkali Soil Plant-Microbial Joint Restoration, College of Life Sciences, Shandong Agricultural University, Shandong, Tai’an 271018, China; Corresponding authorState Key Laboratory of Wheat Improvement, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Engineering Center of Saline-Alkali Soil Plant-Microbial Joint Restoration, College of Life Sciences, Shandong Agricultural University, Shandong, Tai’an 271018, China; Corresponding authorSummary: Although preventing sodium ion import is crucial for salt tolerance, the mechanisms and the Na+ importers involved remain unclear. We identified NRT1.2/NPF4.6/AIT1 as a non-selective Na+ importer. NRT1.2 overexpression in Arabidopsis increased salt sensitivity due to Na+ accumulation, while nrt1.2 mutants exhibited opposite results. Non-invasive micro-test technology indicated Na+ uptake capability of NRT1.2 in Arabidopsis roots. When expressed in Xenopus laevis oocytes, NRT1.2 displayed low-pH-dependent and NO3−-promoted Na+, K+, and Li+ importer capacity, conferring higher potassium and lithium tolerance in NRT1.2 overexpression plants. Mechanically, salt overly sensitive 2 (SOS2) phosphorylated NRT1.2 at Thr248, reducing its Na+ import and preventing excessive Na+ accumulation. Prolonged salt stress also downregulated NRT1.2 transcripts. In summary, our findings unveil a role of NRT1.2 in Na+ transport and a regulatory pathway via SOS2-mediated phosphorylation of NRT1.2 at Thr248, crucial for plant salt-stress adaptation.http://www.sciencedirect.com/science/article/pii/S2211124725005005CP: PlantsCP: Cell biology |
| spellingShingle | Xiaoxiao Liu Lei Zhang Zeqi Zhao Yue Zheng Yue Ren Xiaomin Zhao Shizhong Zhang Guodong Yang Jinguang Huang Kang Yan Chunlong Li Chengchao Zheng Changai Wu Regulation of the non-selective Na+ importer capacity of NRT1.2/NPF4.6/AIT1 by SOS2-mediated phosphorylation in Arabidopsis Cell Reports CP: Plants CP: Cell biology |
| title | Regulation of the non-selective Na+ importer capacity of NRT1.2/NPF4.6/AIT1 by SOS2-mediated phosphorylation in Arabidopsis |
| title_full | Regulation of the non-selective Na+ importer capacity of NRT1.2/NPF4.6/AIT1 by SOS2-mediated phosphorylation in Arabidopsis |
| title_fullStr | Regulation of the non-selective Na+ importer capacity of NRT1.2/NPF4.6/AIT1 by SOS2-mediated phosphorylation in Arabidopsis |
| title_full_unstemmed | Regulation of the non-selective Na+ importer capacity of NRT1.2/NPF4.6/AIT1 by SOS2-mediated phosphorylation in Arabidopsis |
| title_short | Regulation of the non-selective Na+ importer capacity of NRT1.2/NPF4.6/AIT1 by SOS2-mediated phosphorylation in Arabidopsis |
| title_sort | regulation of the non selective na importer capacity of nrt1 2 npf4 6 ait1 by sos2 mediated phosphorylation in arabidopsis |
| topic | CP: Plants CP: Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124725005005 |
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