Molecularly imprinted poly(o-aminophenol)-based electrochemical sensor for the quantitative detection of a VP28 biomarker for white spot syndrome virus
White spot syndrome virus (WSSV) is a deadly pathogen that can cause mass mortality within several days. VP28, known as a biomarker of WSSV, accounts for over half of the total envelope protein. It plays a crucial role in viral infection and is a useful diagnostic indicator for immunological detecti...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-12-01
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| Series: | Sensors and Actuators Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666053925000931 |
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| Summary: | White spot syndrome virus (WSSV) is a deadly pathogen that can cause mass mortality within several days. VP28, known as a biomarker of WSSV, accounts for over half of the total envelope protein. It plays a crucial role in viral infection and is a useful diagnostic indicator for immunological detection.Here, we developed a molecularly imprinted polymer (MIP)-based electrochemical sensor using a gold nanostructured electrode and assessed its performance in detecting VP28. The electropolymerized poly(o-aminophenol) film supplies specific binding sites complementary to VP28. The molecularly imprinted cavities were examined using electrochemical analysis to confirm their ability to recognize the target molecule. The sensor generates a response signal through label-free sensing with a redox probe. It demonstrated a detection limit of 7.01 ng/mL (S/N = 3) and an imprinting factor of 4.25 at 0.78–50 ng/mL concentrations. The selectivity study revealed a response signal 4.5 times higher for the target molecule than for interfering substances. Compared with the standard PCR method for real samples, the MIP-based sensor performed similarly according to virus concentration. These findings suggested that the MIP-based sensor is a simple and suitable alternative to conventional WSSV detection methods. This electrochemical sensing platform with electropolymerization of the molecularly imprinted polymer can promote the detection and monitoring of WSSV expressing VP28. |
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| ISSN: | 2666-0539 |