Peroxisome Proliferator-Activated Receptor γ Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells
Lipid phosphate phosphohydrolase 1 (LPP1), a membrane ectophosphohydrolase regulating the availability of bioactive lipid phosphates, plays important roles in cellular signaling and physiological processes such as angiogenesis and endothelial migration. However, the regulated expression of LPP1 rema...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2014/740121 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832554198469705728 |
|---|---|
| author | Yazi Huang Beilei Zhao Yahan Liu Nanping Wang |
| author_facet | Yazi Huang Beilei Zhao Yahan Liu Nanping Wang |
| author_sort | Yazi Huang |
| collection | DOAJ |
| description | Lipid phosphate phosphohydrolase 1 (LPP1), a membrane ectophosphohydrolase regulating the availability of bioactive lipid phosphates, plays important roles in cellular signaling and physiological processes such as angiogenesis and endothelial migration. However, the regulated expression of LPP1 remains largely unknown. Here, we aimed to examine a role of peroxisome proliferator-activated receptor γ (PPARγ) in the transcriptional control of LPP1 gene expression. In human umbilical vein endothelial cells (HUVECs), quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) demonstrated that activation of PPARγ increased the mRNA level of LPP1. Chromatin immunoprecipitation assay showed that PPARγ binds to the putative PPAR-responsive elements (PPREs) within the 5′-flanking region of the human LPP1 gene. Genomic fragment containing 1.7-kilobase of the promoter region was cloned by using PCR. The luciferase reporter assays demonstrated that overexpression of PPARγ and rosiglitazone, a specific ligand for PPARγ, could significantly upregulate the reporter activity. However, site-directed mutagenesis of the PPRE motif abolished the induction. In conclusion, our results demonstrated that PPARγ transcriptionally activated the expression of LPP1 gene in ECs, suggesting a potential role of PPARγ in the metabolism of phospholipids. |
| format | Article |
| id | doaj-art-0612630a498a4b3f925bdfb549c6a530 |
| institution | Kabale University |
| issn | 1687-4757 1687-4765 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-0612630a498a4b3f925bdfb549c6a5302025-02-03T05:52:09ZengWileyPPAR Research1687-47571687-47652014-01-01201410.1155/2014/740121740121Peroxisome Proliferator-Activated Receptor γ Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial CellsYazi Huang0Beilei Zhao1Yahan Liu2Nanping Wang3Institute of Cardiovascular Science, Peking University Health Science Center, Beijing 100191, ChinaInstitute of Cardiovascular Science, Peking University Health Science Center, Beijing 100191, ChinaInstitute of Cardiovascular Science, Peking University Health Science Center, Beijing 100191, ChinaInstitute of Cardiovascular Science, Peking University Health Science Center, Beijing 100191, ChinaLipid phosphate phosphohydrolase 1 (LPP1), a membrane ectophosphohydrolase regulating the availability of bioactive lipid phosphates, plays important roles in cellular signaling and physiological processes such as angiogenesis and endothelial migration. However, the regulated expression of LPP1 remains largely unknown. Here, we aimed to examine a role of peroxisome proliferator-activated receptor γ (PPARγ) in the transcriptional control of LPP1 gene expression. In human umbilical vein endothelial cells (HUVECs), quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) demonstrated that activation of PPARγ increased the mRNA level of LPP1. Chromatin immunoprecipitation assay showed that PPARγ binds to the putative PPAR-responsive elements (PPREs) within the 5′-flanking region of the human LPP1 gene. Genomic fragment containing 1.7-kilobase of the promoter region was cloned by using PCR. The luciferase reporter assays demonstrated that overexpression of PPARγ and rosiglitazone, a specific ligand for PPARγ, could significantly upregulate the reporter activity. However, site-directed mutagenesis of the PPRE motif abolished the induction. In conclusion, our results demonstrated that PPARγ transcriptionally activated the expression of LPP1 gene in ECs, suggesting a potential role of PPARγ in the metabolism of phospholipids.http://dx.doi.org/10.1155/2014/740121 |
| spellingShingle | Yazi Huang Beilei Zhao Yahan Liu Nanping Wang Peroxisome Proliferator-Activated Receptor γ Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells PPAR Research |
| title | Peroxisome Proliferator-Activated Receptor γ Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells |
| title_full | Peroxisome Proliferator-Activated Receptor γ Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells |
| title_fullStr | Peroxisome Proliferator-Activated Receptor γ Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells |
| title_full_unstemmed | Peroxisome Proliferator-Activated Receptor γ Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells |
| title_short | Peroxisome Proliferator-Activated Receptor γ Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells |
| title_sort | peroxisome proliferator activated receptor γ regulates the expression of lipid phosphate phosphohydrolase 1 in human vascular endothelial cells |
| url | http://dx.doi.org/10.1155/2014/740121 |
| work_keys_str_mv | AT yazihuang peroxisomeproliferatoractivatedreceptorgregulatestheexpressionoflipidphosphatephosphohydrolase1inhumanvascularendothelialcells AT beileizhao peroxisomeproliferatoractivatedreceptorgregulatestheexpressionoflipidphosphatephosphohydrolase1inhumanvascularendothelialcells AT yahanliu peroxisomeproliferatoractivatedreceptorgregulatestheexpressionoflipidphosphatephosphohydrolase1inhumanvascularendothelialcells AT nanpingwang peroxisomeproliferatoractivatedreceptorgregulatestheexpressionoflipidphosphatephosphohydrolase1inhumanvascularendothelialcells |