Methylation of the telomerase gene promoter region in umbilical cord blood of patients with gestational diabetes mellitus is associated with decreased telomerase expression levels and shortened telomere length
ObjectiveThis study speculates that gestational diabetes mellitus (GDM) may reduce fetal telomere length (TL),which may be related to modification of methylation in the promoter region of the telomerase (TE) gene promoter region.MethodsIn this study, umbilical cord blood samples from patients with a...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-03-01
|
| Series: | Frontiers in Endocrinology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2025.1502329/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850034397484417024 |
|---|---|
| author | Shuhua Liu Shuhua Liu Shuhua Liu Liping Xu Liping Xu Yan Cheng Yan Cheng Dehong Liu Dehong Liu Dehong Liu Bin Zhang Bin Zhang Bin Zhang Xianxia Chen Xianxia Chen Xianxia Chen Xianxia Chen Mingming Zheng Mingming Zheng Mingming Zheng |
| author_facet | Shuhua Liu Shuhua Liu Shuhua Liu Liping Xu Liping Xu Yan Cheng Yan Cheng Dehong Liu Dehong Liu Dehong Liu Bin Zhang Bin Zhang Bin Zhang Xianxia Chen Xianxia Chen Xianxia Chen Xianxia Chen Mingming Zheng Mingming Zheng Mingming Zheng |
| author_sort | Shuhua Liu |
| collection | DOAJ |
| description | ObjectiveThis study speculates that gestational diabetes mellitus (GDM) may reduce fetal telomere length (TL),which may be related to modification of methylation in the promoter region of the telomerase (TE) gene promoter region.MethodsIn this study, umbilical cord blood samples from patients with and without GDM (N = 100 each) were analyzed by prospective case-control. The TL, TE expression levels, and methylation levels of TERT and TERC gene promoter regions in two groups were measured. The significance of the methylation level of each CpG locus employed logistic regression analysis of R software, and the analysis of covariance (ANCOVA) was used to control the influence of confounding factors. Correlation analysis was performed by the Spearman.ResultsThe TL and TE expression levels of the offspring of GDM patients were decreased despite adjusting for PBMI, PWG, and TG. A total of two CpG islands were screened in the promoter region of the TERT gene and three fragments (TERT_2, TERT_3, and TERT_4) containing a total of 70 CpG sites were designed. Additionally, four CpG sites of the TERT gene in the GDM group (TERT_2_40, TERT_2_47, TERT_3_46, and TERT_3_212) showed increased methylation levels compared with the control group (all P < 0.05). In the promoter region of the TERC gene, one CpG island containing 19 CpG loci was screened and designed, and the methylation levels of the two CpG sites were significantly different in TERC_1_67 (0.65 ± 0.21 versus 0.57 ± 0.30; P = 0.040) and TERC_1_120 (0.68 ± 0.23 versus 0.59 ± 0.27; P = 0.014). The methylation levels of TERC gene fragments of GDM patients were significantly higher than those of the control group (0.69 ± 0.06 versus 0.65 ± 0.08, P = 0.001).ConclusionThis study revealed that GDM may induce decreased TE expression by increasing the methylation levels of TE genes promoter region, thereby reducing the TL. |
| format | Article |
| id | doaj-art-06095c8a965f48648007d01c5ad2eca8 |
| institution | DOAJ |
| issn | 1664-2392 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj-art-06095c8a965f48648007d01c5ad2eca82025-08-20T02:57:51ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922025-03-011610.3389/fendo.2025.15023291502329Methylation of the telomerase gene promoter region in umbilical cord blood of patients with gestational diabetes mellitus is associated with decreased telomerase expression levels and shortened telomere lengthShuhua Liu0Shuhua Liu1Shuhua Liu2Liping Xu3Liping Xu4Yan Cheng5Yan Cheng6Dehong Liu7Dehong Liu8Dehong Liu9Bin Zhang10Bin Zhang11Bin Zhang12Xianxia Chen13Xianxia Chen14Xianxia Chen15Xianxia Chen16Mingming Zheng17Mingming Zheng18Mingming Zheng19Department of Obstetrics and Gynecology, Hefei Maternal and Child Health Hospital, Hefei, ChinaDepartment of Obstetrics and Gynecology, Anhui Women and Children’s Medical Center, Hefei, ChinaDepartment of Obstetrics and Gynecology, Maternal and Child Medical Center of Anhui Medical University, Hefei, ChinaDepartment of Obstetrics and Gynecology, Maternal and Child Medical Center of Anhui Medical University, Hefei, ChinaFifth School of Clinical Medicine, Anhui Medical University, Hefei, ChinaDepartment of Obstetrics and Gynecology, Maternal and Child Medical Center of Anhui Medical University, Hefei, ChinaFifth School of Clinical Medicine, Anhui Medical University, Hefei, ChinaDepartment of Obstetrics and Gynecology, Hefei Maternal and Child Health Hospital, Hefei, ChinaDepartment of Obstetrics and Gynecology, Anhui Women and Children’s Medical Center, Hefei, ChinaDepartment of Obstetrics and Gynecology, Maternal and Child Medical Center of Anhui Medical University, Hefei, ChinaDepartment of Obstetrics and Gynecology, Hefei Maternal and Child Health Hospital, Hefei, ChinaDepartment of Obstetrics and Gynecology, Anhui Women and Children’s Medical Center, Hefei, ChinaDepartment of Obstetrics and Gynecology, Maternal and Child Medical Center of Anhui Medical University, Hefei, ChinaDepartment of Obstetrics and Gynecology, Hefei Maternal and Child Health Hospital, Hefei, ChinaDepartment of Obstetrics and Gynecology, Anhui Women and Children’s Medical Center, Hefei, ChinaDepartment of Obstetrics and Gynecology, Maternal and Child Medical Center of Anhui Medical University, Hefei, ChinaFifth School of Clinical Medicine, Anhui Medical University, Hefei, ChinaDepartment of Obstetrics and Gynecology, Hefei Maternal and Child Health Hospital, Hefei, ChinaDepartment of Obstetrics and Gynecology, Anhui Women and Children’s Medical Center, Hefei, ChinaDepartment of Obstetrics and Gynecology, Maternal and Child Medical Center of Anhui Medical University, Hefei, ChinaObjectiveThis study speculates that gestational diabetes mellitus (GDM) may reduce fetal telomere length (TL),which may be related to modification of methylation in the promoter region of the telomerase (TE) gene promoter region.MethodsIn this study, umbilical cord blood samples from patients with and without GDM (N = 100 each) were analyzed by prospective case-control. The TL, TE expression levels, and methylation levels of TERT and TERC gene promoter regions in two groups were measured. The significance of the methylation level of each CpG locus employed logistic regression analysis of R software, and the analysis of covariance (ANCOVA) was used to control the influence of confounding factors. Correlation analysis was performed by the Spearman.ResultsThe TL and TE expression levels of the offspring of GDM patients were decreased despite adjusting for PBMI, PWG, and TG. A total of two CpG islands were screened in the promoter region of the TERT gene and three fragments (TERT_2, TERT_3, and TERT_4) containing a total of 70 CpG sites were designed. Additionally, four CpG sites of the TERT gene in the GDM group (TERT_2_40, TERT_2_47, TERT_3_46, and TERT_3_212) showed increased methylation levels compared with the control group (all P < 0.05). In the promoter region of the TERC gene, one CpG island containing 19 CpG loci was screened and designed, and the methylation levels of the two CpG sites were significantly different in TERC_1_67 (0.65 ± 0.21 versus 0.57 ± 0.30; P = 0.040) and TERC_1_120 (0.68 ± 0.23 versus 0.59 ± 0.27; P = 0.014). The methylation levels of TERC gene fragments of GDM patients were significantly higher than those of the control group (0.69 ± 0.06 versus 0.65 ± 0.08, P = 0.001).ConclusionThis study revealed that GDM may induce decreased TE expression by increasing the methylation levels of TE genes promoter region, thereby reducing the TL.https://www.frontiersin.org/articles/10.3389/fendo.2025.1502329/fullgestational diabetes mellitustelomere lengthtelomeraseTERTTERCmethylation |
| spellingShingle | Shuhua Liu Shuhua Liu Shuhua Liu Liping Xu Liping Xu Yan Cheng Yan Cheng Dehong Liu Dehong Liu Dehong Liu Bin Zhang Bin Zhang Bin Zhang Xianxia Chen Xianxia Chen Xianxia Chen Xianxia Chen Mingming Zheng Mingming Zheng Mingming Zheng Methylation of the telomerase gene promoter region in umbilical cord blood of patients with gestational diabetes mellitus is associated with decreased telomerase expression levels and shortened telomere length Frontiers in Endocrinology gestational diabetes mellitus telomere length telomerase TERT TERC methylation |
| title | Methylation of the telomerase gene promoter region in umbilical cord blood of patients with gestational diabetes mellitus is associated with decreased telomerase expression levels and shortened telomere length |
| title_full | Methylation of the telomerase gene promoter region in umbilical cord blood of patients with gestational diabetes mellitus is associated with decreased telomerase expression levels and shortened telomere length |
| title_fullStr | Methylation of the telomerase gene promoter region in umbilical cord blood of patients with gestational diabetes mellitus is associated with decreased telomerase expression levels and shortened telomere length |
| title_full_unstemmed | Methylation of the telomerase gene promoter region in umbilical cord blood of patients with gestational diabetes mellitus is associated with decreased telomerase expression levels and shortened telomere length |
| title_short | Methylation of the telomerase gene promoter region in umbilical cord blood of patients with gestational diabetes mellitus is associated with decreased telomerase expression levels and shortened telomere length |
| title_sort | methylation of the telomerase gene promoter region in umbilical cord blood of patients with gestational diabetes mellitus is associated with decreased telomerase expression levels and shortened telomere length |
| topic | gestational diabetes mellitus telomere length telomerase TERT TERC methylation |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2025.1502329/full |
| work_keys_str_mv | AT shuhualiu methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength AT shuhualiu methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength AT shuhualiu methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength AT lipingxu methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength AT lipingxu methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength AT yancheng methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength AT yancheng methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength AT dehongliu methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength AT dehongliu methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength AT dehongliu methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength AT binzhang methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength AT binzhang methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength AT binzhang methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength AT xianxiachen methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength AT xianxiachen methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength AT xianxiachen methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength AT xianxiachen methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength AT mingmingzheng methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength AT mingmingzheng methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength AT mingmingzheng methylationofthetelomerasegenepromoterregioninumbilicalcordbloodofpatientswithgestationaldiabetesmellitusisassociatedwithdecreasedtelomeraseexpressionlevelsandshortenedtelomerelength |