Flood Inoculation of Fusarium eumartii in Tomato Seedlings: Method for Evaluating the Infectivity of Pathogen Spores
The Fusarium genus includes various fungi of great significance in agriculture. Fusarium solani f. sp. eumartii (F. eumartii), traditionally known as a potato pathogen, has also been identified as a cause of disease in tomatoes. This protocol provides a detailed, efficient, and robust flood-inoculat...
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Bio-protocol LLC
2025-02-01
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| author | María Terrile Florencia Mesas María Picco María Salcedo Andrea Mansilla |
| author_facet | María Terrile Florencia Mesas María Picco María Salcedo Andrea Mansilla |
| author_sort | María Terrile |
| collection | DOAJ |
| description | The Fusarium genus includes various fungi of great significance in agriculture. Fusarium solani f. sp. eumartii (F. eumartii), traditionally known as a potato pathogen, has also been identified as a cause of disease in tomatoes. This protocol provides a detailed, efficient, and robust flood-inoculation method for assessing F. eumartii infection of young tomato seedlings grown on MS medium plates. It includes the evaluation of the lesion area and the quantification of the remaining fungal inoculum in tomato seedlings. In summary, the straightforwardness and efficiency of this bioassay make it a powerful quantitative tool for selecting fungicidal compounds or defense response inducers in tomato plants, offering a promising approach with significant potential for preventing fungal diseases in crops. |
| format | Article |
| id | doaj-art-060798a66e2c416a9610ff0ebce1c6d7 |
| institution | Kabale University |
| issn | 2331-8325 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Bio-protocol LLC |
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| spelling | doaj-art-060798a66e2c416a9610ff0ebce1c6d72025-02-07T08:16:47ZengBio-protocol LLCBio-Protocol2331-83252025-02-0115310.21769/BioProtoc.5198Flood Inoculation of Fusarium eumartii in Tomato Seedlings: Method for Evaluating the Infectivity of Pathogen SporesMaría Terrile0Florencia Mesas1María Picco2María Salcedo3Andrea Mansilla4Instituto de Investigaciones Biológicas (IIB), UE CONICET-UNMDP, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, ArgentinaInstituto de Investigaciones Biológicas (IIB), UE CONICET-UNMDP, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, ArgentinaInstituto de Investigaciones Biológicas (IIB), UE CONICET-UNMDP, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, ArgentinaInstituto de Investigaciones en Ciencia y Tecnología de Materiales (INTEMA) UE CONICET-UNMdP, Facultad de Ingeniería, Universidad Nacional de Mar del Plata, Mar del Plata, ArgentinaInstituto de Investigaciones Biológicas (IIB), UE CONICET-UNMDP, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, ArgentinaThe Fusarium genus includes various fungi of great significance in agriculture. Fusarium solani f. sp. eumartii (F. eumartii), traditionally known as a potato pathogen, has also been identified as a cause of disease in tomatoes. This protocol provides a detailed, efficient, and robust flood-inoculation method for assessing F. eumartii infection of young tomato seedlings grown on MS medium plates. It includes the evaluation of the lesion area and the quantification of the remaining fungal inoculum in tomato seedlings. In summary, the straightforwardness and efficiency of this bioassay make it a powerful quantitative tool for selecting fungicidal compounds or defense response inducers in tomato plants, offering a promising approach with significant potential for preventing fungal diseases in crops.https://bio-protocol.org/en/bpdetail?id=5198&type=0 |
| spellingShingle | María Terrile Florencia Mesas María Picco María Salcedo Andrea Mansilla Flood Inoculation of Fusarium eumartii in Tomato Seedlings: Method for Evaluating the Infectivity of Pathogen Spores Bio-Protocol |
| title | Flood Inoculation of Fusarium eumartii in Tomato Seedlings: Method for Evaluating the Infectivity of Pathogen Spores |
| title_full | Flood Inoculation of Fusarium eumartii in Tomato Seedlings: Method for Evaluating the Infectivity of Pathogen Spores |
| title_fullStr | Flood Inoculation of Fusarium eumartii in Tomato Seedlings: Method for Evaluating the Infectivity of Pathogen Spores |
| title_full_unstemmed | Flood Inoculation of Fusarium eumartii in Tomato Seedlings: Method for Evaluating the Infectivity of Pathogen Spores |
| title_short | Flood Inoculation of Fusarium eumartii in Tomato Seedlings: Method for Evaluating the Infectivity of Pathogen Spores |
| title_sort | flood inoculation of fusarium eumartii in tomato seedlings method for evaluating the infectivity of pathogen spores |
| url | https://bio-protocol.org/en/bpdetail?id=5198&type=0 |
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