Molecular and Microbial Detections of Streptococcus mutans and Lactobacilli in Dental Caries: An Experimental Study on Iranian Children Aged 5–9
ABSTRACT Objectives Lactobacilli and Streptococcus mutans are stigmatized as cariogenic bacteria, but few studies have simultaneously examined the quantitative and qualitative aspects of lactobacilli and S. mutans in childhood dental caries. Therefore, this study aimed to detect the presence of S. m...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-12-01
|
| Series: | Clinical and Experimental Dental Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cre2.70039 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850249345845166080 |
|---|---|
| author | Marzieh Danaei Milad Mollaali Vida Fakharmohialdini Hamidreza Poureslami Fatemeh Sadat Sajadi Elham Farokh Gisour Fatemeh Jahanimoghadam Aida Gholampour Mehrnaz Foroudisefat Arezoo Mirshekari Raziyeh Shojaeipour |
| author_facet | Marzieh Danaei Milad Mollaali Vida Fakharmohialdini Hamidreza Poureslami Fatemeh Sadat Sajadi Elham Farokh Gisour Fatemeh Jahanimoghadam Aida Gholampour Mehrnaz Foroudisefat Arezoo Mirshekari Raziyeh Shojaeipour |
| author_sort | Marzieh Danaei |
| collection | DOAJ |
| description | ABSTRACT Objectives Lactobacilli and Streptococcus mutans are stigmatized as cariogenic bacteria, but few studies have simultaneously examined the quantitative and qualitative aspects of lactobacilli and S. mutans in childhood dental caries. Therefore, this study aimed to detect the presence of S. mutans and lactobacilli in dental caries using Snyder's test, colony counting, and PCR in the primary teeth of Iranian children with dental caries. Material and Methods A cross‐sectional study was conducted in Kerman, Iran, from March to Jun 2024. After dental examinations, 120 eligible children aged 5–9 were chosen using simple random sampling and classified into four groups based on their decayed, missing, and filled (primary) teeth (dmft) index: Group 1 (dmft = 0), Group 2 (dmft = 4–6), Group 3 (dmft = 7–9), and Group 4 (dmft = 10–13). The salivary levels of lactobacilli and S. mutans were calculated using colony counting (CFU/ml). Moreover, Snyder's test was applied to evaluate caries activity. PCR was also performed for molecular detection of lactobacilli (16S rRNA gene) and S. mutans (gftB gene). Lastly, the association between bacterial counting, molecular findings, and Snyder's test was estimated through statistical methods using SPSS 27. Results Significant differences were found between the age and the PCR results of lactobacilli and S. mutans among all dmft Groups (p < 0.05). Moreover, positive significant correlations were observed between the counts of lactobacilli and S. mutans in dmft Group 1 compared to other dmft Groups (p < 0.05). Furthermore, the counts of these two bacteria differed significantly in Snyder's test (p < 0.05). However, Snyder's test differed significantly from the S. mutans PCR (p < 0.05), but not from the lactobacilli PCR (p > 0.05). Conclusions The results of the study could potentially be considered a promising and cost‐effective screening program to identify children who are susceptible to dental caries. |
| format | Article |
| id | doaj-art-05f783a963614a1ca567da63fbf4a457 |
| institution | OA Journals |
| issn | 2057-4347 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Experimental Dental Research |
| spelling | doaj-art-05f783a963614a1ca567da63fbf4a4572025-08-20T01:58:31ZengWileyClinical and Experimental Dental Research2057-43472024-12-01106n/an/a10.1002/cre2.70039Molecular and Microbial Detections of Streptococcus mutans and Lactobacilli in Dental Caries: An Experimental Study on Iranian Children Aged 5–9Marzieh Danaei0Milad Mollaali1Vida Fakharmohialdini2Hamidreza Poureslami3Fatemeh Sadat Sajadi4Elham Farokh Gisour5Fatemeh Jahanimoghadam6Aida Gholampour7Mehrnaz Foroudisefat8Arezoo Mirshekari9Raziyeh Shojaeipour10Dana Gene Pajoohan Karmania Company, Member of Iran High‐Tech Laboratory Network Kerman IranDana Gene Pajoohan Karmania Company, Member of Iran High‐Tech Laboratory Network Kerman IranDana Gene Pajoohan Karmania Company, Member of Iran High‐Tech Laboratory Network Kerman IranOral and Dental Diseases Research Center, Department of Pediatrics Dentistry, School of Dentistry Kerman University of Medical Sciences Kerman IranOral and Dental Diseases Research Center, Department of Pediatrics Dentistry, School of Dentistry Kerman University of Medical Sciences Kerman IranOral and Dental Diseases Research Center, Department of Pediatrics Dentistry, School of Dentistry Kerman University of Medical Sciences Kerman IranSocial Determinants on Oral Health Research Center Kerman University of Medical Sciences Kerman IranDepartment of Pediatrics Dentistry, School of Dentistry Kerman University of Medical Sciences Kerman IranDepartment of Pediatrics Dentistry, School of Dentistry Kerman University of Medical Sciences Kerman IranDepartment of Pediatrics Dentistry, School of Dentistry Kerman University of Medical Sciences Kerman IranOral and Dental Diseases Research Center, Department of Pediatrics Dentistry, School of Dentistry Kerman University of Medical Sciences Kerman IranABSTRACT Objectives Lactobacilli and Streptococcus mutans are stigmatized as cariogenic bacteria, but few studies have simultaneously examined the quantitative and qualitative aspects of lactobacilli and S. mutans in childhood dental caries. Therefore, this study aimed to detect the presence of S. mutans and lactobacilli in dental caries using Snyder's test, colony counting, and PCR in the primary teeth of Iranian children with dental caries. Material and Methods A cross‐sectional study was conducted in Kerman, Iran, from March to Jun 2024. After dental examinations, 120 eligible children aged 5–9 were chosen using simple random sampling and classified into four groups based on their decayed, missing, and filled (primary) teeth (dmft) index: Group 1 (dmft = 0), Group 2 (dmft = 4–6), Group 3 (dmft = 7–9), and Group 4 (dmft = 10–13). The salivary levels of lactobacilli and S. mutans were calculated using colony counting (CFU/ml). Moreover, Snyder's test was applied to evaluate caries activity. PCR was also performed for molecular detection of lactobacilli (16S rRNA gene) and S. mutans (gftB gene). Lastly, the association between bacterial counting, molecular findings, and Snyder's test was estimated through statistical methods using SPSS 27. Results Significant differences were found between the age and the PCR results of lactobacilli and S. mutans among all dmft Groups (p < 0.05). Moreover, positive significant correlations were observed between the counts of lactobacilli and S. mutans in dmft Group 1 compared to other dmft Groups (p < 0.05). Furthermore, the counts of these two bacteria differed significantly in Snyder's test (p < 0.05). However, Snyder's test differed significantly from the S. mutans PCR (p < 0.05), but not from the lactobacilli PCR (p > 0.05). Conclusions The results of the study could potentially be considered a promising and cost‐effective screening program to identify children who are susceptible to dental caries.https://doi.org/10.1002/cre2.70039Bacterial loadDental care for childrenDental cariesDental caries activity tests |
| spellingShingle | Marzieh Danaei Milad Mollaali Vida Fakharmohialdini Hamidreza Poureslami Fatemeh Sadat Sajadi Elham Farokh Gisour Fatemeh Jahanimoghadam Aida Gholampour Mehrnaz Foroudisefat Arezoo Mirshekari Raziyeh Shojaeipour Molecular and Microbial Detections of Streptococcus mutans and Lactobacilli in Dental Caries: An Experimental Study on Iranian Children Aged 5–9 Clinical and Experimental Dental Research Bacterial load Dental care for children Dental caries Dental caries activity tests |
| title | Molecular and Microbial Detections of Streptococcus mutans and Lactobacilli in Dental Caries: An Experimental Study on Iranian Children Aged 5–9 |
| title_full | Molecular and Microbial Detections of Streptococcus mutans and Lactobacilli in Dental Caries: An Experimental Study on Iranian Children Aged 5–9 |
| title_fullStr | Molecular and Microbial Detections of Streptococcus mutans and Lactobacilli in Dental Caries: An Experimental Study on Iranian Children Aged 5–9 |
| title_full_unstemmed | Molecular and Microbial Detections of Streptococcus mutans and Lactobacilli in Dental Caries: An Experimental Study on Iranian Children Aged 5–9 |
| title_short | Molecular and Microbial Detections of Streptococcus mutans and Lactobacilli in Dental Caries: An Experimental Study on Iranian Children Aged 5–9 |
| title_sort | molecular and microbial detections of streptococcus mutans and lactobacilli in dental caries an experimental study on iranian children aged 5 9 |
| topic | Bacterial load Dental care for children Dental caries Dental caries activity tests |
| url | https://doi.org/10.1002/cre2.70039 |
| work_keys_str_mv | AT marziehdanaei molecularandmicrobialdetectionsofstreptococcusmutansandlactobacilliindentalcariesanexperimentalstudyoniranianchildrenaged59 AT miladmollaali molecularandmicrobialdetectionsofstreptococcusmutansandlactobacilliindentalcariesanexperimentalstudyoniranianchildrenaged59 AT vidafakharmohialdini molecularandmicrobialdetectionsofstreptococcusmutansandlactobacilliindentalcariesanexperimentalstudyoniranianchildrenaged59 AT hamidrezapoureslami molecularandmicrobialdetectionsofstreptococcusmutansandlactobacilliindentalcariesanexperimentalstudyoniranianchildrenaged59 AT fatemehsadatsajadi molecularandmicrobialdetectionsofstreptococcusmutansandlactobacilliindentalcariesanexperimentalstudyoniranianchildrenaged59 AT elhamfarokhgisour molecularandmicrobialdetectionsofstreptococcusmutansandlactobacilliindentalcariesanexperimentalstudyoniranianchildrenaged59 AT fatemehjahanimoghadam molecularandmicrobialdetectionsofstreptococcusmutansandlactobacilliindentalcariesanexperimentalstudyoniranianchildrenaged59 AT aidagholampour molecularandmicrobialdetectionsofstreptococcusmutansandlactobacilliindentalcariesanexperimentalstudyoniranianchildrenaged59 AT mehrnazforoudisefat molecularandmicrobialdetectionsofstreptococcusmutansandlactobacilliindentalcariesanexperimentalstudyoniranianchildrenaged59 AT arezoomirshekari molecularandmicrobialdetectionsofstreptococcusmutansandlactobacilliindentalcariesanexperimentalstudyoniranianchildrenaged59 AT raziyehshojaeipour molecularandmicrobialdetectionsofstreptococcusmutansandlactobacilliindentalcariesanexperimentalstudyoniranianchildrenaged59 |