Ameliorative Effect of <i>Sipunculus nudus</i> Hydrolysate on Cisplatin-Induced Nephrotoxicity by Mitigating Oxidative Stress, Inflammation and Apoptosis
The present study investigated the protective effects and possible mechanisms of an ultrafiltration fraction of <i>Sipunculus nudus</i> hydrolysate (UFSH) on cisplatin-induced kidney damage in a mouse model. The results showed that UFSH significantly attenuated cisplatin-induced nephroto...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/23/3/100 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850091155139592192 |
|---|---|
| author | Susu Tao Yi Qi Jialong Gao Huafang Yuan Ruimin Wang Xiaoqin Shen Gang Wei Zhilan Peng |
| author_facet | Susu Tao Yi Qi Jialong Gao Huafang Yuan Ruimin Wang Xiaoqin Shen Gang Wei Zhilan Peng |
| author_sort | Susu Tao |
| collection | DOAJ |
| description | The present study investigated the protective effects and possible mechanisms of an ultrafiltration fraction of <i>Sipunculus nudus</i> hydrolysate (UFSH) on cisplatin-induced kidney damage in a mouse model. The results showed that UFSH significantly attenuated cisplatin-induced nephrotoxicity by inhibiting increases in blood urea nitrogen (BUN) and serum creatinine (SCr). Additionally, UFSH treatment significantly alleviated cisplatin-induced renal histopathological changes, such as significant dilation of renal tubules, cast formation, and tubular cell necrosis, as well as tubulointerstitial fibrosis. Moreover, UFSH decreased cisplatin-induced oxidative stress by increasing the activities of antioxidant enzymes SOD and GSH-Px, while reducing the malondialdehyde (MDA) level in the kidney. Furthermore, UFSH significantly inhibited cisplatin-induced increases in inflammatory cytokines, including Interleukin 1-beta (IL-1β), Interleukin-6 (IL-6), and Tumor necrosis factor-alpha (TNF-α). Western blotting revealed that UFSH inhibited the phosphorylation of the inflammation-associated MAPK/NF-κB signaling pathway, lowered the expression of the apoptosis-related protein Bax, and reversed the reduction in the anti-apoptotic Bcl-2 protein. This investigation demonstrated that UFSH can ameliorate cisplatin-induced nephrotoxicity by mitigating oxidative stress, inflammation, and apoptosis. |
| format | Article |
| id | doaj-art-05eb1ce61a3546ada9df8dc03cf806eb |
| institution | DOAJ |
| issn | 1660-3397 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj-art-05eb1ce61a3546ada9df8dc03cf806eb2025-08-20T02:42:26ZengMDPI AGMarine Drugs1660-33972025-02-0123310010.3390/md23030100Ameliorative Effect of <i>Sipunculus nudus</i> Hydrolysate on Cisplatin-Induced Nephrotoxicity by Mitigating Oxidative Stress, Inflammation and ApoptosisSusu Tao0Yi Qi1Jialong Gao2Huafang Yuan3Ruimin Wang4Xiaoqin Shen5Gang Wei6Zhilan Peng7School of Ocean and Tropical Medicine, Guangdong Medical University, Zhanjiang 524023, ChinaSchool of Ocean and Tropical Medicine, Guangdong Medical University, Zhanjiang 524023, ChinaCollege of Food Science & Technology, Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Zhanjiang 524088, ChinaThe Marine Biomedical Research Institute of Guangdong Zhanjiang, Zhanjiang 524023, ChinaThe Marine Biomedical Research Institute of Guangdong Zhanjiang, Zhanjiang 524023, ChinaThe Marine Biomedical Research Institute of Guangdong Zhanjiang, Zhanjiang 524023, ChinaSchool of Ocean and Tropical Medicine, Guangdong Medical University, Zhanjiang 524023, ChinaSchool of Ocean and Tropical Medicine, Guangdong Medical University, Zhanjiang 524023, ChinaThe present study investigated the protective effects and possible mechanisms of an ultrafiltration fraction of <i>Sipunculus nudus</i> hydrolysate (UFSH) on cisplatin-induced kidney damage in a mouse model. The results showed that UFSH significantly attenuated cisplatin-induced nephrotoxicity by inhibiting increases in blood urea nitrogen (BUN) and serum creatinine (SCr). Additionally, UFSH treatment significantly alleviated cisplatin-induced renal histopathological changes, such as significant dilation of renal tubules, cast formation, and tubular cell necrosis, as well as tubulointerstitial fibrosis. Moreover, UFSH decreased cisplatin-induced oxidative stress by increasing the activities of antioxidant enzymes SOD and GSH-Px, while reducing the malondialdehyde (MDA) level in the kidney. Furthermore, UFSH significantly inhibited cisplatin-induced increases in inflammatory cytokines, including Interleukin 1-beta (IL-1β), Interleukin-6 (IL-6), and Tumor necrosis factor-alpha (TNF-α). Western blotting revealed that UFSH inhibited the phosphorylation of the inflammation-associated MAPK/NF-κB signaling pathway, lowered the expression of the apoptosis-related protein Bax, and reversed the reduction in the anti-apoptotic Bcl-2 protein. This investigation demonstrated that UFSH can ameliorate cisplatin-induced nephrotoxicity by mitigating oxidative stress, inflammation, and apoptosis.https://www.mdpi.com/1660-3397/23/3/100<i>Sipunculus nudus</i>cisplatinnephrotoxicityhydrolysateoxidative stressinflammation |
| spellingShingle | Susu Tao Yi Qi Jialong Gao Huafang Yuan Ruimin Wang Xiaoqin Shen Gang Wei Zhilan Peng Ameliorative Effect of <i>Sipunculus nudus</i> Hydrolysate on Cisplatin-Induced Nephrotoxicity by Mitigating Oxidative Stress, Inflammation and Apoptosis Marine Drugs <i>Sipunculus nudus</i> cisplatin nephrotoxicity hydrolysate oxidative stress inflammation |
| title | Ameliorative Effect of <i>Sipunculus nudus</i> Hydrolysate on Cisplatin-Induced Nephrotoxicity by Mitigating Oxidative Stress, Inflammation and Apoptosis |
| title_full | Ameliorative Effect of <i>Sipunculus nudus</i> Hydrolysate on Cisplatin-Induced Nephrotoxicity by Mitigating Oxidative Stress, Inflammation and Apoptosis |
| title_fullStr | Ameliorative Effect of <i>Sipunculus nudus</i> Hydrolysate on Cisplatin-Induced Nephrotoxicity by Mitigating Oxidative Stress, Inflammation and Apoptosis |
| title_full_unstemmed | Ameliorative Effect of <i>Sipunculus nudus</i> Hydrolysate on Cisplatin-Induced Nephrotoxicity by Mitigating Oxidative Stress, Inflammation and Apoptosis |
| title_short | Ameliorative Effect of <i>Sipunculus nudus</i> Hydrolysate on Cisplatin-Induced Nephrotoxicity by Mitigating Oxidative Stress, Inflammation and Apoptosis |
| title_sort | ameliorative effect of i sipunculus nudus i hydrolysate on cisplatin induced nephrotoxicity by mitigating oxidative stress inflammation and apoptosis |
| topic | <i>Sipunculus nudus</i> cisplatin nephrotoxicity hydrolysate oxidative stress inflammation |
| url | https://www.mdpi.com/1660-3397/23/3/100 |
| work_keys_str_mv | AT susutao ameliorativeeffectofisipunculusnudusihydrolysateoncisplatininducednephrotoxicitybymitigatingoxidativestressinflammationandapoptosis AT yiqi ameliorativeeffectofisipunculusnudusihydrolysateoncisplatininducednephrotoxicitybymitigatingoxidativestressinflammationandapoptosis AT jialonggao ameliorativeeffectofisipunculusnudusihydrolysateoncisplatininducednephrotoxicitybymitigatingoxidativestressinflammationandapoptosis AT huafangyuan ameliorativeeffectofisipunculusnudusihydrolysateoncisplatininducednephrotoxicitybymitigatingoxidativestressinflammationandapoptosis AT ruiminwang ameliorativeeffectofisipunculusnudusihydrolysateoncisplatininducednephrotoxicitybymitigatingoxidativestressinflammationandapoptosis AT xiaoqinshen ameliorativeeffectofisipunculusnudusihydrolysateoncisplatininducednephrotoxicitybymitigatingoxidativestressinflammationandapoptosis AT gangwei ameliorativeeffectofisipunculusnudusihydrolysateoncisplatininducednephrotoxicitybymitigatingoxidativestressinflammationandapoptosis AT zhilanpeng ameliorativeeffectofisipunculusnudusihydrolysateoncisplatininducednephrotoxicitybymitigatingoxidativestressinflammationandapoptosis |