Retrospective review: single- and multidonor washed microbiota transplantation have equivalent efficacy in the treatment of autism
BackgroundAutism spectrum disorder (ASD) is a serious neurodevelopmental disorder with no effective treatment. This study explored the short-term clinical effects of washed microbiota transplantation (WMT) with different numbers of donors on autism.MethodsConsecutive ASD patients treated with two co...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1606417/full |
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| author | Ya-Mei Zheng Meng-Meng Ye Hong-Ying Zhang Dan-Ping Luo Tao Liu Xing-Xiang He Xian-Yun Chen Li-Hao Wu |
| author_facet | Ya-Mei Zheng Meng-Meng Ye Hong-Ying Zhang Dan-Ping Luo Tao Liu Xing-Xiang He Xian-Yun Chen Li-Hao Wu |
| author_sort | Ya-Mei Zheng |
| collection | DOAJ |
| description | BackgroundAutism spectrum disorder (ASD) is a serious neurodevelopmental disorder with no effective treatment. This study explored the short-term clinical effects of washed microbiota transplantation (WMT) with different numbers of donors on autism.MethodsConsecutive ASD patients treated with two continuous WMT courses from March 2020 to March 2022 at the First Affiliated Hospital of Guangdong Pharmaceutical University were retrospectively assessed. Basic information, aberrant behavior checklist (ABC) scores, childhood autism rating scale (CARS) scores, sleep disturbance scale for children (SDSC) scores, adverse reactions, and feces were collected.ResultsForty-four patients were included (single-donor group: 17 patients; multidonor group: 27 patients). The CARS, ABC and SDSC scores didn’t differ between the two groups before treatment. After two courses, the scores for the 44 patients were lower than those at baseline (P<0.05), with no severe adverse reactions observed. After the first course, the mean ABC (P=0.049) and SDSC (P=0.019) scores were significantly different between the single-donor and multidonor groups, but the difference disappeared after two courses. The alpha-diversity of the faecal flora in the effective-group was greater than that in the ineffective-group (Shannon index P=0.0018). Lactobacillus was the predominant genus in the effective group, whereas Faecalibacterium, Campylobacter, and Sphingomonas were predominant genera in the ineffective group.ConclusionAfter two WMT courses, the symptoms of ASD improved, with good short-term treatment efficacy. The ASD symptom improvement did not differ between the single-donor and multidonor groups. Changes in the alpha-diversity and abundance of the faecal microbiota after WMT may be related to treatment efficacy. |
| format | Article |
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| institution | OA Journals |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-05e7a1bec39247aa96d5f2e88f13c3e82025-08-20T02:36:16ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-07-011510.3389/fcimb.2025.16064171606417Retrospective review: single- and multidonor washed microbiota transplantation have equivalent efficacy in the treatment of autismYa-Mei Zheng0Meng-Meng Ye1Hong-Ying Zhang2Dan-Ping Luo3Tao Liu4Xing-Xiang He5Xian-Yun Chen6Li-Hao Wu7Department of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Research Center for Engineering Techniques of Microbiota-Targeted Therapies of Guangdong Province, Guangdong, Guangzhou, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Research Center for Engineering Techniques of Microbiota-Targeted Therapies of Guangdong Province, Guangdong, Guangzhou, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Research Center for Engineering Techniques of Microbiota-Targeted Therapies of Guangdong Province, Guangdong, Guangzhou, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Research Center for Engineering Techniques of Microbiota-Targeted Therapies of Guangdong Province, Guangdong, Guangzhou, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Research Center for Engineering Techniques of Microbiota-Targeted Therapies of Guangdong Province, Guangdong, Guangzhou, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Research Center for Engineering Techniques of Microbiota-Targeted Therapies of Guangdong Province, Guangdong, Guangzhou, ChinaDepartment of Gastroenterology, The First People's Hospital of Shaoguan, Shaoguan, Guangdong, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Research Center for Engineering Techniques of Microbiota-Targeted Therapies of Guangdong Province, Guangdong, Guangzhou, ChinaBackgroundAutism spectrum disorder (ASD) is a serious neurodevelopmental disorder with no effective treatment. This study explored the short-term clinical effects of washed microbiota transplantation (WMT) with different numbers of donors on autism.MethodsConsecutive ASD patients treated with two continuous WMT courses from March 2020 to March 2022 at the First Affiliated Hospital of Guangdong Pharmaceutical University were retrospectively assessed. Basic information, aberrant behavior checklist (ABC) scores, childhood autism rating scale (CARS) scores, sleep disturbance scale for children (SDSC) scores, adverse reactions, and feces were collected.ResultsForty-four patients were included (single-donor group: 17 patients; multidonor group: 27 patients). The CARS, ABC and SDSC scores didn’t differ between the two groups before treatment. After two courses, the scores for the 44 patients were lower than those at baseline (P<0.05), with no severe adverse reactions observed. After the first course, the mean ABC (P=0.049) and SDSC (P=0.019) scores were significantly different between the single-donor and multidonor groups, but the difference disappeared after two courses. The alpha-diversity of the faecal flora in the effective-group was greater than that in the ineffective-group (Shannon index P=0.0018). Lactobacillus was the predominant genus in the effective group, whereas Faecalibacterium, Campylobacter, and Sphingomonas were predominant genera in the ineffective group.ConclusionAfter two WMT courses, the symptoms of ASD improved, with good short-term treatment efficacy. The ASD symptom improvement did not differ between the single-donor and multidonor groups. Changes in the alpha-diversity and abundance of the faecal microbiota after WMT may be related to treatment efficacy.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1606417/fullwashed microbiota transplantationautismshort-term efficacyfaecal florasingle donormultidonor |
| spellingShingle | Ya-Mei Zheng Meng-Meng Ye Hong-Ying Zhang Dan-Ping Luo Tao Liu Xing-Xiang He Xian-Yun Chen Li-Hao Wu Retrospective review: single- and multidonor washed microbiota transplantation have equivalent efficacy in the treatment of autism Frontiers in Cellular and Infection Microbiology washed microbiota transplantation autism short-term efficacy faecal flora single donor multidonor |
| title | Retrospective review: single- and multidonor washed microbiota transplantation have equivalent efficacy in the treatment of autism |
| title_full | Retrospective review: single- and multidonor washed microbiota transplantation have equivalent efficacy in the treatment of autism |
| title_fullStr | Retrospective review: single- and multidonor washed microbiota transplantation have equivalent efficacy in the treatment of autism |
| title_full_unstemmed | Retrospective review: single- and multidonor washed microbiota transplantation have equivalent efficacy in the treatment of autism |
| title_short | Retrospective review: single- and multidonor washed microbiota transplantation have equivalent efficacy in the treatment of autism |
| title_sort | retrospective review single and multidonor washed microbiota transplantation have equivalent efficacy in the treatment of autism |
| topic | washed microbiota transplantation autism short-term efficacy faecal flora single donor multidonor |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1606417/full |
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