Combined application of pectin and Bacillus spp. in the diets of rohu, Labeo rohita (Hamilton): Effects on growth, feed utilization, immunity, haemato-biochemical profile and pathogen resistance
Probiotics, prebiotics, and synbiotics have drawn attention to enhance growth, immunity, and disease resistance in fish. This study evaluated the single or combinatorial effects of pectin (1 %) and Bacillus safensis or B. amyloliquefaciens (7 Log CFU g−1 feed) on rohu, Labeo rohita fingerlings. The...
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Elsevier
2025-09-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425002716 |
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| author | Tanaya Sukul Koushik Ghosh |
| author_facet | Tanaya Sukul Koushik Ghosh |
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| description | Probiotics, prebiotics, and synbiotics have drawn attention to enhance growth, immunity, and disease resistance in fish. This study evaluated the single or combinatorial effects of pectin (1 %) and Bacillus safensis or B. amyloliquefaciens (7 Log CFU g−1 feed) on rohu, Labeo rohita fingerlings. The basal diet without pectin or bacilli was the control (C). The basal diet fortified with pectin (E1), B. safensis (E2), B. amyloliquefaciens (E3), B. safensis + pectin (E4), and B. amyloliquefaciens + pectin (E5) were fed to the experimental fish (6.4 ± 0.2 g) for 90 days in triplicate. Overall, the results demonstrated significant (p < 0.05) improvement in weight gain (%) and feed efficiency with the combined application of B. safensis and pectin (E4) compared to the control and only probiotic/prebiotic fed groups. Improved activities of digestive (protease, lipase) and glycolytic enzymes (glucokinase, hexokinase) were noticed with dietary synbiotics administration. Further, synbiotics-fed groups (E4 and E5) demonstrated significant (p < 0.05) improvements in immune parameters (serum lysozyme, antiprotease, phagocytosis, respiratory burst, peroxidase, and complement activity), erythrocytes, haemoglobin content and serum protein compared to the control group. Following 90 days of feeding, pathogenic Aeromonas sobria was intra-peritoneally injected into the fish, and information on stress along with immune parameters was recorded. Results depicted that the application of pectin together with B. safensis or B. amyloliquefaciens improved the innate immunity and survivability of the challenged fish. Further, increased superoxide dismutase and glutathione peroxidase activities might indicate the fish's resilience to oxidative stress. Fish fed a diet containing B. safensis and pectin had the highest post-challenge survival rate, suggesting prospective application of this specific combination as functional feed additive to improve growth, immunity, and pathogen resistance in L. rohita. |
| format | Article |
| id | doaj-art-05e5bfc8ddd64328a59b4c42e448034d |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
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| series | Aquaculture Reports |
| spelling | doaj-art-05e5bfc8ddd64328a59b4c42e448034d2025-08-20T04:00:27ZengElsevierAquaculture Reports2352-51342025-09-014310288510.1016/j.aqrep.2025.102885Combined application of pectin and Bacillus spp. in the diets of rohu, Labeo rohita (Hamilton): Effects on growth, feed utilization, immunity, haemato-biochemical profile and pathogen resistanceTanaya Sukul0Koushik Ghosh1Aquaculture Laboratory, Department of Zoology, The University of Burdwan, Golapbag, Burdwan, West Bengal 713104, IndiaCorresponding author.; Aquaculture Laboratory, Department of Zoology, The University of Burdwan, Golapbag, Burdwan, West Bengal 713104, IndiaProbiotics, prebiotics, and synbiotics have drawn attention to enhance growth, immunity, and disease resistance in fish. This study evaluated the single or combinatorial effects of pectin (1 %) and Bacillus safensis or B. amyloliquefaciens (7 Log CFU g−1 feed) on rohu, Labeo rohita fingerlings. The basal diet without pectin or bacilli was the control (C). The basal diet fortified with pectin (E1), B. safensis (E2), B. amyloliquefaciens (E3), B. safensis + pectin (E4), and B. amyloliquefaciens + pectin (E5) were fed to the experimental fish (6.4 ± 0.2 g) for 90 days in triplicate. Overall, the results demonstrated significant (p < 0.05) improvement in weight gain (%) and feed efficiency with the combined application of B. safensis and pectin (E4) compared to the control and only probiotic/prebiotic fed groups. Improved activities of digestive (protease, lipase) and glycolytic enzymes (glucokinase, hexokinase) were noticed with dietary synbiotics administration. Further, synbiotics-fed groups (E4 and E5) demonstrated significant (p < 0.05) improvements in immune parameters (serum lysozyme, antiprotease, phagocytosis, respiratory burst, peroxidase, and complement activity), erythrocytes, haemoglobin content and serum protein compared to the control group. Following 90 days of feeding, pathogenic Aeromonas sobria was intra-peritoneally injected into the fish, and information on stress along with immune parameters was recorded. Results depicted that the application of pectin together with B. safensis or B. amyloliquefaciens improved the innate immunity and survivability of the challenged fish. Further, increased superoxide dismutase and glutathione peroxidase activities might indicate the fish's resilience to oxidative stress. Fish fed a diet containing B. safensis and pectin had the highest post-challenge survival rate, suggesting prospective application of this specific combination as functional feed additive to improve growth, immunity, and pathogen resistance in L. rohita.http://www.sciencedirect.com/science/article/pii/S2352513425002716ProbioticSynbioticPectinCarpStressAeromonas sobria |
| spellingShingle | Tanaya Sukul Koushik Ghosh Combined application of pectin and Bacillus spp. in the diets of rohu, Labeo rohita (Hamilton): Effects on growth, feed utilization, immunity, haemato-biochemical profile and pathogen resistance Aquaculture Reports Probiotic Synbiotic Pectin Carp Stress Aeromonas sobria |
| title | Combined application of pectin and Bacillus spp. in the diets of rohu, Labeo rohita (Hamilton): Effects on growth, feed utilization, immunity, haemato-biochemical profile and pathogen resistance |
| title_full | Combined application of pectin and Bacillus spp. in the diets of rohu, Labeo rohita (Hamilton): Effects on growth, feed utilization, immunity, haemato-biochemical profile and pathogen resistance |
| title_fullStr | Combined application of pectin and Bacillus spp. in the diets of rohu, Labeo rohita (Hamilton): Effects on growth, feed utilization, immunity, haemato-biochemical profile and pathogen resistance |
| title_full_unstemmed | Combined application of pectin and Bacillus spp. in the diets of rohu, Labeo rohita (Hamilton): Effects on growth, feed utilization, immunity, haemato-biochemical profile and pathogen resistance |
| title_short | Combined application of pectin and Bacillus spp. in the diets of rohu, Labeo rohita (Hamilton): Effects on growth, feed utilization, immunity, haemato-biochemical profile and pathogen resistance |
| title_sort | combined application of pectin and bacillus spp in the diets of rohu labeo rohita hamilton effects on growth feed utilization immunity haemato biochemical profile and pathogen resistance |
| topic | Probiotic Synbiotic Pectin Carp Stress Aeromonas sobria |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425002716 |
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