Characterization of Genetic Determinants Involved in Antimicrobial Resistance in <em>Aeromonas hydrophila</em>, <em>Escherichia coli</em> and <em>Vibrio cholerae</em> Isolated from Different Aquatic Environments
<i>Aeromonas hydrophila</i>, <i>Escherichia coli</i> and <i>Vibrio cholerae</i> are among a myriad of bacteria pathogen commonly found in natural water bodies that cause serious waterborne infection while antibiotic resistance genes are emerging contaminants posin...
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MDPI AG
2020-08-01
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| author | Iroha I. Romanus Ude Ibiam Ejikeugwu C. Peter Onochie C. Chike |
| author_facet | Iroha I. Romanus Ude Ibiam Ejikeugwu C. Peter Onochie C. Chike |
| author_sort | Iroha I. Romanus |
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| description | <i>Aeromonas hydrophila</i>, <i>Escherichia coli</i> and <i>Vibrio cholerae</i> are among a myriad of bacteria pathogen commonly found in natural water bodies that cause serious waterborne infection while antibiotic resistance genes are emerging contaminants posing potential worldwide human health risk. This study was designed to determined genetic determinants involved in antimicrobial resistance in bacteria isolates from aquatic environments. A total of 372 water samples, comprising of 111, 144 and 117 ponds, rivers and streams were collected from three local governments areas (Abakaliki, Ebonyi and Ikwo) of Ebonyi State Nigeria over a period of twelve (12) months. Bacteria Isolates obtained from water bodies were identified and characterized by polymerase chain reaction (PCR) analysis using 16S rRNA specific primers. The susceptibility of the isolates to different antibiotics was determined using disc diffusion technique. Total DNA was extracted and sequenced on Genetic Analyzer 3130 xl sequencer and the amplified 16S rRNA gene sequence. The presence of antibiotic resistance genes was determined by PCR using specific primers. Bacteria isolated were <i>Aeromonas hydrophila</i> (103), <i>Escherichia coli</i> (118) and <i>Vibrio cholera</i> (87). The isolates were susceptibility to gentamicin (96.12%), meropenem (94.17%), ciprofloxacin (89.32%) imipenem (85.44%) and chloramphenicol (84.47%) while they showed 94.17%, 93.20%, 90.29%, 89.32%, 88.35%, 86.41% and 83.5% resistance to penicillin, sulphamethoxazole, kanamycin, azithromycin, streptomycin cephalothin and cefuroxime respectively. The isolates were found to harbor the following antibiotic resistance genes, blaCTXM, blaSHV, class 1 Integron Ac, Intl1, blaTEM and TetB. These findings deserve serious attention, as the presence of bacteria coliforms in water harbouring antibiotic resistance genes are potential risk to the health of people. Keywords: aquatic environment resistant genes characterization. |
| format | Article |
| id | doaj-art-05dceb6245be4a5b91cd6b2b9cf46b55 |
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| issn | 2504-3900 |
| language | English |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-05dceb6245be4a5b91cd6b2b9cf46b552025-08-20T02:23:32ZengMDPI AGProceedings2504-39002020-08-014511510.3390/proceedings2020045015Characterization of Genetic Determinants Involved in Antimicrobial Resistance in <em>Aeromonas hydrophila</em>, <em>Escherichia coli</em> and <em>Vibrio cholerae</em> Isolated from Different Aquatic EnvironmentsIroha I. Romanus0Ude Ibiam1Ejikeugwu C. Peter2Onochie C. Chike3Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki 480214, NigeriaDepartment of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki 480214, NigeriaDepartment of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki 480214, NigeriaDepartment of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki 480214, Nigeria<i>Aeromonas hydrophila</i>, <i>Escherichia coli</i> and <i>Vibrio cholerae</i> are among a myriad of bacteria pathogen commonly found in natural water bodies that cause serious waterborne infection while antibiotic resistance genes are emerging contaminants posing potential worldwide human health risk. This study was designed to determined genetic determinants involved in antimicrobial resistance in bacteria isolates from aquatic environments. A total of 372 water samples, comprising of 111, 144 and 117 ponds, rivers and streams were collected from three local governments areas (Abakaliki, Ebonyi and Ikwo) of Ebonyi State Nigeria over a period of twelve (12) months. Bacteria Isolates obtained from water bodies were identified and characterized by polymerase chain reaction (PCR) analysis using 16S rRNA specific primers. The susceptibility of the isolates to different antibiotics was determined using disc diffusion technique. Total DNA was extracted and sequenced on Genetic Analyzer 3130 xl sequencer and the amplified 16S rRNA gene sequence. The presence of antibiotic resistance genes was determined by PCR using specific primers. Bacteria isolated were <i>Aeromonas hydrophila</i> (103), <i>Escherichia coli</i> (118) and <i>Vibrio cholera</i> (87). The isolates were susceptibility to gentamicin (96.12%), meropenem (94.17%), ciprofloxacin (89.32%) imipenem (85.44%) and chloramphenicol (84.47%) while they showed 94.17%, 93.20%, 90.29%, 89.32%, 88.35%, 86.41% and 83.5% resistance to penicillin, sulphamethoxazole, kanamycin, azithromycin, streptomycin cephalothin and cefuroxime respectively. The isolates were found to harbor the following antibiotic resistance genes, blaCTXM, blaSHV, class 1 Integron Ac, Intl1, blaTEM and TetB. These findings deserve serious attention, as the presence of bacteria coliforms in water harbouring antibiotic resistance genes are potential risk to the health of people. Keywords: aquatic environment resistant genes characterization.https://www.mdpi.com/2504-3900/45/1/15n/a |
| spellingShingle | Iroha I. Romanus Ude Ibiam Ejikeugwu C. Peter Onochie C. Chike Characterization of Genetic Determinants Involved in Antimicrobial Resistance in <em>Aeromonas hydrophila</em>, <em>Escherichia coli</em> and <em>Vibrio cholerae</em> Isolated from Different Aquatic Environments Proceedings n/a |
| title | Characterization of Genetic Determinants Involved in Antimicrobial Resistance in <em>Aeromonas hydrophila</em>, <em>Escherichia coli</em> and <em>Vibrio cholerae</em> Isolated from Different Aquatic Environments |
| title_full | Characterization of Genetic Determinants Involved in Antimicrobial Resistance in <em>Aeromonas hydrophila</em>, <em>Escherichia coli</em> and <em>Vibrio cholerae</em> Isolated from Different Aquatic Environments |
| title_fullStr | Characterization of Genetic Determinants Involved in Antimicrobial Resistance in <em>Aeromonas hydrophila</em>, <em>Escherichia coli</em> and <em>Vibrio cholerae</em> Isolated from Different Aquatic Environments |
| title_full_unstemmed | Characterization of Genetic Determinants Involved in Antimicrobial Resistance in <em>Aeromonas hydrophila</em>, <em>Escherichia coli</em> and <em>Vibrio cholerae</em> Isolated from Different Aquatic Environments |
| title_short | Characterization of Genetic Determinants Involved in Antimicrobial Resistance in <em>Aeromonas hydrophila</em>, <em>Escherichia coli</em> and <em>Vibrio cholerae</em> Isolated from Different Aquatic Environments |
| title_sort | characterization of genetic determinants involved in antimicrobial resistance in em aeromonas hydrophila em em escherichia coli em and em vibrio cholerae em isolated from different aquatic environments |
| topic | n/a |
| url | https://www.mdpi.com/2504-3900/45/1/15 |
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