Nestin Reporter Transgene Labels Multiple Central Nervous System Precursor Cells
Embryonic neuroepithelia and adult subventricular zone (SVZ) stem and progenitor cells express nestin. We characterized a transgenic line that expresses enhanced green fluorescent protein (eGFP) specified to neural tissue by the second intronic enhancer of the nestin promoter that had several novel...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2010/894374 |
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| author | Avery S. Walker Gwendolyn E. Goings Yongsoo Kim Richard J. Miller Anjen Chenn Francis G. Szele |
| author_facet | Avery S. Walker Gwendolyn E. Goings Yongsoo Kim Richard J. Miller Anjen Chenn Francis G. Szele |
| author_sort | Avery S. Walker |
| collection | DOAJ |
| description | Embryonic neuroepithelia and adult subventricular zone (SVZ) stem and progenitor cells express nestin. We characterized a transgenic line that expresses enhanced green fluorescent protein (eGFP) specified to neural tissue by the second intronic enhancer of the nestin promoter that had several novel features. During embryogenesis, the dorsal telencephalon contained many and the ventral telencephalon few eGFP+ cells. eGFP+ cells were found in postnatal and adult neurogenic regions. eGFP+ cells in the SVZ expressed multiple phenotype markers, glial fibrillary acidic protein, Dlx, and neuroblast-specific molecules suggesting the transgene is expressed through the lineage. eGFP+ cell numbers increased in the SVZ after cortical injury, suggesting this line will be useful in probing postinjury neurogenesis. In non-neurogenic regions, eGFP was strongly expressed in oligodendrocyte progenitors, but not in astrocytes, even when they were reactive. This eGFP+ mouse will facilitate studies of proliferative neuroepithelia and adult neurogenesis, as well as of parenchymal oligodendrocytes. |
| format | Article |
| id | doaj-art-05d9281c6cbc478b90ef8e200a641b65 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-05d9281c6cbc478b90ef8e200a641b652025-02-03T01:00:53ZengWileyNeural Plasticity2090-59041687-54432010-01-01201010.1155/2010/894374894374Nestin Reporter Transgene Labels Multiple Central Nervous System Precursor CellsAvery S. Walker0Gwendolyn E. Goings1Yongsoo Kim2Richard J. Miller3Anjen Chenn4Francis G. Szele5Department of Pediatrics, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611-3008, USADepartment of Pediatrics, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611-3008, USADepartment of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UKDepartment of Molecular Pharmacology and Biological Chemistry, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611-3008, USADepartment of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611-3008, USADepartment of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UKEmbryonic neuroepithelia and adult subventricular zone (SVZ) stem and progenitor cells express nestin. We characterized a transgenic line that expresses enhanced green fluorescent protein (eGFP) specified to neural tissue by the second intronic enhancer of the nestin promoter that had several novel features. During embryogenesis, the dorsal telencephalon contained many and the ventral telencephalon few eGFP+ cells. eGFP+ cells were found in postnatal and adult neurogenic regions. eGFP+ cells in the SVZ expressed multiple phenotype markers, glial fibrillary acidic protein, Dlx, and neuroblast-specific molecules suggesting the transgene is expressed through the lineage. eGFP+ cell numbers increased in the SVZ after cortical injury, suggesting this line will be useful in probing postinjury neurogenesis. In non-neurogenic regions, eGFP was strongly expressed in oligodendrocyte progenitors, but not in astrocytes, even when they were reactive. This eGFP+ mouse will facilitate studies of proliferative neuroepithelia and adult neurogenesis, as well as of parenchymal oligodendrocytes.http://dx.doi.org/10.1155/2010/894374 |
| spellingShingle | Avery S. Walker Gwendolyn E. Goings Yongsoo Kim Richard J. Miller Anjen Chenn Francis G. Szele Nestin Reporter Transgene Labels Multiple Central Nervous System Precursor Cells Neural Plasticity |
| title | Nestin Reporter Transgene Labels Multiple Central Nervous System Precursor Cells |
| title_full | Nestin Reporter Transgene Labels Multiple Central Nervous System Precursor Cells |
| title_fullStr | Nestin Reporter Transgene Labels Multiple Central Nervous System Precursor Cells |
| title_full_unstemmed | Nestin Reporter Transgene Labels Multiple Central Nervous System Precursor Cells |
| title_short | Nestin Reporter Transgene Labels Multiple Central Nervous System Precursor Cells |
| title_sort | nestin reporter transgene labels multiple central nervous system precursor cells |
| url | http://dx.doi.org/10.1155/2010/894374 |
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