Araçá-Boi Extract and Gallic Acid Reduce Cell Viability and Modify the Expression of Tumor Suppressor Genes and Genes Involved in Epigenetic Processes in Ovarian Cancer
In the present study, we characterized and investigated the effect of the araçá-boi extract on antioxidant activity, cell viability, and the regulation of genes related to tumor suppression and epigenetic mechanisms in ovarian cancer cells. The results showed that araçá-boi extract revealed a remark...
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MDPI AG
2025-05-01
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| author | Felipe Tecchio Borsoi Henrique Silvano Arruda Amanda Cristina Andrade Mônica Pezenatto dos Santos Isabelle Nogueira da Silva Leonardo Augusto Marson Ana Sofia Martelli Chaib Saliba Severino Matias de Alencar Murilo Vieira Geraldo Iramaia Angélica Neri Numa Glaucia Maria Pastore |
| author_facet | Felipe Tecchio Borsoi Henrique Silvano Arruda Amanda Cristina Andrade Mônica Pezenatto dos Santos Isabelle Nogueira da Silva Leonardo Augusto Marson Ana Sofia Martelli Chaib Saliba Severino Matias de Alencar Murilo Vieira Geraldo Iramaia Angélica Neri Numa Glaucia Maria Pastore |
| author_sort | Felipe Tecchio Borsoi |
| collection | DOAJ |
| description | In the present study, we characterized and investigated the effect of the araçá-boi extract on antioxidant activity, cell viability, and the regulation of genes related to tumor suppression and epigenetic mechanisms in ovarian cancer cells. The results showed that araçá-boi extract revealed a remarkable diversity of phytochemicals (organic acids, phenolic acids, and flavonoids), significant antioxidant potential, and efficient scavenging of reactive oxygen species, particularly hydroxyl and peroxyl radicals. Gallic acid, one of the phenolic acids present in the extract, was used alone to verify its contribution to cytotoxic activities. Exposure of human ovarian cancer cells (NCI/ADR-RES and OVCAR3) to the extract (0.15–150 μg/mL) and gallic acid (6–48 μg/mL) resulted in a significant reduction in cell viability, particularly after 48 h of treatment. Both treatments modulated genes involved in DNA repair, tumor suppression, and epigenetic regulation. However, no changes were observed in the methylation status of the <i>BRCA1</i> gene promoter region with either araçá-boi extract or gallic acid. These findings reinforce the therapeutic potential of araçá-boi extract and its phenolic compounds against ovarian cancer and point to the need for further studies to better elucidate the molecular pathways involved and validate these effects in vivo. |
| format | Article |
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| institution | OA Journals |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-05ca1f2ea73b43f4aeef1d4cf1729c2e2025-08-20T02:33:11ZengMDPI AGPlants2223-77472025-05-011411167110.3390/plants14111671Araçá-Boi Extract and Gallic Acid Reduce Cell Viability and Modify the Expression of Tumor Suppressor Genes and Genes Involved in Epigenetic Processes in Ovarian CancerFelipe Tecchio Borsoi0Henrique Silvano Arruda1Amanda Cristina Andrade2Mônica Pezenatto dos Santos3Isabelle Nogueira da Silva4Leonardo Augusto Marson5Ana Sofia Martelli Chaib Saliba6Severino Matias de Alencar7Murilo Vieira Geraldo8Iramaia Angélica Neri Numa9Glaucia Maria Pastore10Department of Food Science and Nutrition (DECAN), School of Food Engineering (FEA), University of Campinas (UNICAMP), Monteiro Lobato Street 80, Campinas 13083-862, São Paulo, BrazilDepartment of Food Science and Nutrition (DECAN), School of Food Engineering (FEA), University of Campinas (UNICAMP), Monteiro Lobato Street 80, Campinas 13083-862, São Paulo, BrazilDepartment of Food Science and Nutrition (DECAN), School of Food Engineering (FEA), University of Campinas (UNICAMP), Monteiro Lobato Street 80, Campinas 13083-862, São Paulo, BrazilDepartment of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas 13083-863, São Paulo, BrazilDepartment of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas 13083-863, São Paulo, BrazilDepartment of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas 13083-863, São Paulo, BrazilDepartment of Agri-Food Industry, Food and Nutrition, “Luiz de Queiroz” College of Agriculture (ESALQ), University of São Paulo (USP), Piracicaba 13418-900, São Paulo, BrazilDepartment of Agri-Food Industry, Food and Nutrition, “Luiz de Queiroz” College of Agriculture (ESALQ), University of São Paulo (USP), Piracicaba 13418-900, São Paulo, BrazilDepartment of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas 13083-863, São Paulo, BrazilDepartment of Food Science and Nutrition (DECAN), School of Food Engineering (FEA), University of Campinas (UNICAMP), Monteiro Lobato Street 80, Campinas 13083-862, São Paulo, BrazilDepartment of Food Science and Nutrition (DECAN), School of Food Engineering (FEA), University of Campinas (UNICAMP), Monteiro Lobato Street 80, Campinas 13083-862, São Paulo, BrazilIn the present study, we characterized and investigated the effect of the araçá-boi extract on antioxidant activity, cell viability, and the regulation of genes related to tumor suppression and epigenetic mechanisms in ovarian cancer cells. The results showed that araçá-boi extract revealed a remarkable diversity of phytochemicals (organic acids, phenolic acids, and flavonoids), significant antioxidant potential, and efficient scavenging of reactive oxygen species, particularly hydroxyl and peroxyl radicals. Gallic acid, one of the phenolic acids present in the extract, was used alone to verify its contribution to cytotoxic activities. Exposure of human ovarian cancer cells (NCI/ADR-RES and OVCAR3) to the extract (0.15–150 μg/mL) and gallic acid (6–48 μg/mL) resulted in a significant reduction in cell viability, particularly after 48 h of treatment. Both treatments modulated genes involved in DNA repair, tumor suppression, and epigenetic regulation. However, no changes were observed in the methylation status of the <i>BRCA1</i> gene promoter region with either araçá-boi extract or gallic acid. These findings reinforce the therapeutic potential of araçá-boi extract and its phenolic compounds against ovarian cancer and point to the need for further studies to better elucidate the molecular pathways involved and validate these effects in vivo.https://www.mdpi.com/2223-7747/14/11/1671<i>Eugenia stipitata</i>phenolic extractgene expression<i>BRCA1</i>DNA methylation |
| spellingShingle | Felipe Tecchio Borsoi Henrique Silvano Arruda Amanda Cristina Andrade Mônica Pezenatto dos Santos Isabelle Nogueira da Silva Leonardo Augusto Marson Ana Sofia Martelli Chaib Saliba Severino Matias de Alencar Murilo Vieira Geraldo Iramaia Angélica Neri Numa Glaucia Maria Pastore Araçá-Boi Extract and Gallic Acid Reduce Cell Viability and Modify the Expression of Tumor Suppressor Genes and Genes Involved in Epigenetic Processes in Ovarian Cancer Plants <i>Eugenia stipitata</i> phenolic extract gene expression <i>BRCA1</i> DNA methylation |
| title | Araçá-Boi Extract and Gallic Acid Reduce Cell Viability and Modify the Expression of Tumor Suppressor Genes and Genes Involved in Epigenetic Processes in Ovarian Cancer |
| title_full | Araçá-Boi Extract and Gallic Acid Reduce Cell Viability and Modify the Expression of Tumor Suppressor Genes and Genes Involved in Epigenetic Processes in Ovarian Cancer |
| title_fullStr | Araçá-Boi Extract and Gallic Acid Reduce Cell Viability and Modify the Expression of Tumor Suppressor Genes and Genes Involved in Epigenetic Processes in Ovarian Cancer |
| title_full_unstemmed | Araçá-Boi Extract and Gallic Acid Reduce Cell Viability and Modify the Expression of Tumor Suppressor Genes and Genes Involved in Epigenetic Processes in Ovarian Cancer |
| title_short | Araçá-Boi Extract and Gallic Acid Reduce Cell Viability and Modify the Expression of Tumor Suppressor Genes and Genes Involved in Epigenetic Processes in Ovarian Cancer |
| title_sort | araca boi extract and gallic acid reduce cell viability and modify the expression of tumor suppressor genes and genes involved in epigenetic processes in ovarian cancer |
| topic | <i>Eugenia stipitata</i> phenolic extract gene expression <i>BRCA1</i> DNA methylation |
| url | https://www.mdpi.com/2223-7747/14/11/1671 |
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