Characterization and genomic analysis of Lactiplantibacillus plantarum LP8 as a probiotic candidate for medical applicationsNCBI

This study presents the whole genome sequencing (WGS) and functional analysis of Lactiplantibacillus plantarum LP8, a promising probiotic strain, is presented in this research. The genome comprises a 3.23 Mbp circular chromosome and three plasmids including plasmid1_LP8 (58,764 bp), plasmid2_LP8 (45...

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Bibliographic Details
Main Authors: Nirusna Jehma, Nattarika Chaichana, Jirasa Boonsan, Kamonnut Singkhamanan, Monwadee Wonglapsuwan, Rattanaruji Pomwised, Sarunyou Chusri, Komwit Surachat
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Data in Brief
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Online Access:http://www.sciencedirect.com/science/article/pii/S2352340925002872
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Summary:This study presents the whole genome sequencing (WGS) and functional analysis of Lactiplantibacillus plantarum LP8, a promising probiotic strain, is presented in this research. The genome comprises a 3.23 Mbp circular chromosome and three plasmids including plasmid1_LP8 (58,764 bp), plasmid2_LP8 (45,003 bp), and plasmid3_LP8 (7985 bp). Functional annotation identified 3151 coding sequences (CDSs) mapped to 209 RAST subsystems, alongside biosynthesis gene clusters encoding Plantaricin J and other secondary metabolites such as RiPP-like peptides and terpenes. Safety analysis revealed no acquired antimicrobial resistance gene (AMR) or virulence genes, with only intrinsic resistance to certain antibiotics. In vitro analysis confirmed its sensitivity to antibiotics such as ampicillin, erythromycin, and tetracycline, and its hemolytic activity displayed an α-hemoly-sis pattern. These findings confirm L. plantarum LP8 as a safeand effective probiotic candidate with significant potential for antimicrobial and biotechnological applications.
ISSN:2352-3409