Soy Glycinin Contains a Functional Inhibitory Sequence against Muscle-Atrophy-Associated Ubiquitin Ligase Cbl-b

Background. Unloading stress induces skeletal muscle atrophy. We have reported that Cbl-b ubiquitin ligase is a master regulator of unloading-associated muscle atrophy. The present study was designed to elucidate whether dietary soy glycinin protein prevents denervation-mediated muscle atrophy, base...

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Main Authors: Tomoki Abe, Shohei Kohno, Tomonari Yama, Arisa Ochi, Takuro Suto, Katsuya Hirasaka, Ayako Ohno, Shigetada Teshima-Kondo, Yuushi Okumura, Motoko Oarada, Inho Choi, Rie Mukai, Junji Terao, Takeshi Nikawa
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:International Journal of Endocrinology
Online Access:http://dx.doi.org/10.1155/2013/907565
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author Tomoki Abe
Shohei Kohno
Tomonari Yama
Arisa Ochi
Takuro Suto
Katsuya Hirasaka
Ayako Ohno
Shigetada Teshima-Kondo
Yuushi Okumura
Motoko Oarada
Inho Choi
Rie Mukai
Junji Terao
Takeshi Nikawa
author_facet Tomoki Abe
Shohei Kohno
Tomonari Yama
Arisa Ochi
Takuro Suto
Katsuya Hirasaka
Ayako Ohno
Shigetada Teshima-Kondo
Yuushi Okumura
Motoko Oarada
Inho Choi
Rie Mukai
Junji Terao
Takeshi Nikawa
author_sort Tomoki Abe
collection DOAJ
description Background. Unloading stress induces skeletal muscle atrophy. We have reported that Cbl-b ubiquitin ligase is a master regulator of unloading-associated muscle atrophy. The present study was designed to elucidate whether dietary soy glycinin protein prevents denervation-mediated muscle atrophy, based on the presence of inhibitory peptides against Cbl-b ubiquitin ligase in soy glycinin protein. Methods. Mice were fed either 20% casein diet, 20% soy protein isolate diet, 10% glycinin diet containing 10% casein, or 20% glycinin diet. One week later, the right sciatic nerve was cut. The wet weight, cross sectional area (CSA), IGF-1 signaling, and atrogene expression in hindlimb muscles were examined at 1, 3, 3.5, or 4 days after denervation. Results. 20% soy glycinin diet significantly prevented denervation-induced decreases in muscle wet weight and myofiber CSA. Furthermore, dietary soy protein inhibited denervation-induced ubiquitination and degradation of IRS-1 in tibialis anterior muscle. Dietary soy glycinin partially suppressed the denervation-mediated expression of atrogenes, such as MAFbx/atrogin-1 and MuRF-1, through the protection of IGF-1 signaling estimated by phosphorylation of Akt-1. Conclusions. Soy glycinin contains a functional inhibitory sequence against muscle-atrophy-associated ubiquitin ligase Cbl-b. Dietary soy glycinin protein significantly prevented muscle atrophy after denervation in mice.
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spelling doaj-art-05bfca4eff8645a79d5cc6bbbb090ea62025-08-20T02:02:57ZengWileyInternational Journal of Endocrinology1687-83371687-83452013-01-01201310.1155/2013/907565907565Soy Glycinin Contains a Functional Inhibitory Sequence against Muscle-Atrophy-Associated Ubiquitin Ligase Cbl-bTomoki Abe0Shohei Kohno1Tomonari Yama2Arisa Ochi3Takuro Suto4Katsuya Hirasaka5Ayako Ohno6Shigetada Teshima-Kondo7Yuushi Okumura8Motoko Oarada9Inho Choi10Rie Mukai11Junji Terao12Takeshi Nikawa13Department of Nutritional Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, JapanDepartment of Nutritional Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, JapanDepartment of Nutritional Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, JapanDepartment of Nutritional Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, JapanDepartment of Nutritional Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, JapanDepartment of Nutritional Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, JapanDepartment of Nutritional Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, JapanDepartment of Nutritional Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, JapanDepartment of Nutritional Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, JapanMedical Mycology Research Center, The University of Chiba, Chiba 260-8673, JapanDivision of Biological Science and Technology, College of Science and Technology, Institute of Biomaterials, The University of Yonsei, Wonju 220-710, Republic of KoreaDepartment of Food Science, Institute of Health Biosciences, The University of Tokushima, Tokushima 770-8503, JapanDepartment of Food Science, Institute of Health Biosciences, The University of Tokushima, Tokushima 770-8503, JapanDepartment of Nutritional Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, JapanBackground. Unloading stress induces skeletal muscle atrophy. We have reported that Cbl-b ubiquitin ligase is a master regulator of unloading-associated muscle atrophy. The present study was designed to elucidate whether dietary soy glycinin protein prevents denervation-mediated muscle atrophy, based on the presence of inhibitory peptides against Cbl-b ubiquitin ligase in soy glycinin protein. Methods. Mice were fed either 20% casein diet, 20% soy protein isolate diet, 10% glycinin diet containing 10% casein, or 20% glycinin diet. One week later, the right sciatic nerve was cut. The wet weight, cross sectional area (CSA), IGF-1 signaling, and atrogene expression in hindlimb muscles were examined at 1, 3, 3.5, or 4 days after denervation. Results. 20% soy glycinin diet significantly prevented denervation-induced decreases in muscle wet weight and myofiber CSA. Furthermore, dietary soy protein inhibited denervation-induced ubiquitination and degradation of IRS-1 in tibialis anterior muscle. Dietary soy glycinin partially suppressed the denervation-mediated expression of atrogenes, such as MAFbx/atrogin-1 and MuRF-1, through the protection of IGF-1 signaling estimated by phosphorylation of Akt-1. Conclusions. Soy glycinin contains a functional inhibitory sequence against muscle-atrophy-associated ubiquitin ligase Cbl-b. Dietary soy glycinin protein significantly prevented muscle atrophy after denervation in mice.http://dx.doi.org/10.1155/2013/907565
spellingShingle Tomoki Abe
Shohei Kohno
Tomonari Yama
Arisa Ochi
Takuro Suto
Katsuya Hirasaka
Ayako Ohno
Shigetada Teshima-Kondo
Yuushi Okumura
Motoko Oarada
Inho Choi
Rie Mukai
Junji Terao
Takeshi Nikawa
Soy Glycinin Contains a Functional Inhibitory Sequence against Muscle-Atrophy-Associated Ubiquitin Ligase Cbl-b
International Journal of Endocrinology
title Soy Glycinin Contains a Functional Inhibitory Sequence against Muscle-Atrophy-Associated Ubiquitin Ligase Cbl-b
title_full Soy Glycinin Contains a Functional Inhibitory Sequence against Muscle-Atrophy-Associated Ubiquitin Ligase Cbl-b
title_fullStr Soy Glycinin Contains a Functional Inhibitory Sequence against Muscle-Atrophy-Associated Ubiquitin Ligase Cbl-b
title_full_unstemmed Soy Glycinin Contains a Functional Inhibitory Sequence against Muscle-Atrophy-Associated Ubiquitin Ligase Cbl-b
title_short Soy Glycinin Contains a Functional Inhibitory Sequence against Muscle-Atrophy-Associated Ubiquitin Ligase Cbl-b
title_sort soy glycinin contains a functional inhibitory sequence against muscle atrophy associated ubiquitin ligase cbl b
url http://dx.doi.org/10.1155/2013/907565
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