Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering
Concentrations of S-Adenosyl-L-Methionine (SAM) in aqueous solution and fermentation liquids were quantitatively determined by surface-enhanced Raman scattering (SERS) and verified by high-pressure liquid chromatography (HPLC). The Ag nanoparticle/silicon nanowire array substrate was fabricated and...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2016/4910630 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832546106036191232 |
|---|---|
| author | Hairui Ren Zhaoyang Chen Xin Zhang Yongmei Zhao Zheng Wang Zhenglong Wu Haijun Xu |
| author_facet | Hairui Ren Zhaoyang Chen Xin Zhang Yongmei Zhao Zheng Wang Zhenglong Wu Haijun Xu |
| author_sort | Hairui Ren |
| collection | DOAJ |
| description | Concentrations of S-Adenosyl-L-Methionine (SAM) in aqueous solution and fermentation liquids were quantitatively determined by surface-enhanced Raman scattering (SERS) and verified by high-pressure liquid chromatography (HPLC). The Ag nanoparticle/silicon nanowire array substrate was fabricated and employed as an active SERS substrate to indirectly measure the SAM concentration. The linear relationship between the integrated intensity of peak centered at ~2920 cm−1 in SERS spectra and the SAM concentration was established, and the limit of detections of SAM concentrations was analyzed to be ~0.1 g/L. The concentration of SAM in real solution could be predicted by the linear relationship and verified by the HPLC detection method. The relative deviations (δ) of the predicted SAM concentration are less than 13% and the correlation coefficient is 0.9998. Rolling-Circle Filter was utilized to subtract fluorescence background and the optimal results were obtained when the radius of the analyzing circle is 650 cm−1. |
| format | Article |
| id | doaj-art-05bdeea78aae4c0794d7665f3f354746 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-05bdeea78aae4c0794d7665f3f3547462025-02-03T07:23:52ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732016-01-01201610.1155/2016/49106304910630Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman ScatteringHairui Ren0Zhaoyang Chen1Xin Zhang2Yongmei Zhao3Zheng Wang4Zhenglong Wu5Haijun Xu6Beijing Bioprocess Key Laboratory, Beijing University of Chemical Technology, Beijing 100029, ChinaBeijing Bioprocess Key Laboratory, Beijing University of Chemical Technology, Beijing 100029, ChinaBeijing Bioprocess Key Laboratory, Beijing University of Chemical Technology, Beijing 100029, ChinaEngineering Research Center for Semiconductor Integrated Technology, Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, ChinaBeijing Bioprocess Key Laboratory, Beijing University of Chemical Technology, Beijing 100029, ChinaAnalytical and Testing Center, Beijing Normal University, Beijing 100875, ChinaBeijing Bioprocess Key Laboratory, Beijing University of Chemical Technology, Beijing 100029, ChinaConcentrations of S-Adenosyl-L-Methionine (SAM) in aqueous solution and fermentation liquids were quantitatively determined by surface-enhanced Raman scattering (SERS) and verified by high-pressure liquid chromatography (HPLC). The Ag nanoparticle/silicon nanowire array substrate was fabricated and employed as an active SERS substrate to indirectly measure the SAM concentration. The linear relationship between the integrated intensity of peak centered at ~2920 cm−1 in SERS spectra and the SAM concentration was established, and the limit of detections of SAM concentrations was analyzed to be ~0.1 g/L. The concentration of SAM in real solution could be predicted by the linear relationship and verified by the HPLC detection method. The relative deviations (δ) of the predicted SAM concentration are less than 13% and the correlation coefficient is 0.9998. Rolling-Circle Filter was utilized to subtract fluorescence background and the optimal results were obtained when the radius of the analyzing circle is 650 cm−1.http://dx.doi.org/10.1155/2016/4910630 |
| spellingShingle | Hairui Ren Zhaoyang Chen Xin Zhang Yongmei Zhao Zheng Wang Zhenglong Wu Haijun Xu Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering Journal of Analytical Methods in Chemistry |
| title | Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering |
| title_full | Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering |
| title_fullStr | Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering |
| title_full_unstemmed | Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering |
| title_short | Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering |
| title_sort | rapid and quantitative determination of s adenosyl l methionine in the fermentation process by surface enhanced raman scattering |
| url | http://dx.doi.org/10.1155/2016/4910630 |
| work_keys_str_mv | AT hairuiren rapidandquantitativedeterminationofsadenosyllmethionineinthefermentationprocessbysurfaceenhancedramanscattering AT zhaoyangchen rapidandquantitativedeterminationofsadenosyllmethionineinthefermentationprocessbysurfaceenhancedramanscattering AT xinzhang rapidandquantitativedeterminationofsadenosyllmethionineinthefermentationprocessbysurfaceenhancedramanscattering AT yongmeizhao rapidandquantitativedeterminationofsadenosyllmethionineinthefermentationprocessbysurfaceenhancedramanscattering AT zhengwang rapidandquantitativedeterminationofsadenosyllmethionineinthefermentationprocessbysurfaceenhancedramanscattering AT zhenglongwu rapidandquantitativedeterminationofsadenosyllmethionineinthefermentationprocessbysurfaceenhancedramanscattering AT haijunxu rapidandquantitativedeterminationofsadenosyllmethionineinthefermentationprocessbysurfaceenhancedramanscattering |