Allotetraploid Origin and Putative Ancient Introgression in Plantago hakusanensis (Plantaginaceae)
ABSTRACT Plantago hakusanensis (2n = 4x = 24) is an endangered endemic species that occurs in subalpine zones in Japan. To clarify the unresolved phylogenetic position of P. hakusanensis within subgenus Plantago, we constructed a phylogenetic tree based on the nuclear‐encoded single‐copy gene sucros...
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| Format: | Article |
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Wiley
2025-03-01
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| Series: | Ecology and Evolution |
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| Online Access: | https://doi.org/10.1002/ece3.71144 |
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| author | Naoko Ishikawa Shota Sakaguchi Chikako Hasekura Alexey Shipunov Ayumi Matsuo Yoshihisa Suyama Hirokazu Tsukaya Hiroshi Ikeda Motomi Ito |
| author_facet | Naoko Ishikawa Shota Sakaguchi Chikako Hasekura Alexey Shipunov Ayumi Matsuo Yoshihisa Suyama Hirokazu Tsukaya Hiroshi Ikeda Motomi Ito |
| author_sort | Naoko Ishikawa |
| collection | DOAJ |
| description | ABSTRACT Plantago hakusanensis (2n = 4x = 24) is an endangered endemic species that occurs in subalpine zones in Japan. To clarify the unresolved phylogenetic position of P. hakusanensis within subgenus Plantago, we constructed a phylogenetic tree based on the nuclear‐encoded single‐copy gene sucrose–proton symporter 1 (SUC1) using 60 previously reported alleles from 24 taxa in subgenus Plantago. We found that P. hakusanensis was closely related to Plantago asiatica var. densiuscula. The phylogenetic relationships between P. hakusanensis and P. asiatica var. densiuscula were further examined by analyses of the SUC1 nuclear regions and the internal transcribed spacer (ITS) of rDNA, genome‐wide single‐nucleotide polymorphism genotyping (via multiplexed inter‐simple sequence repeat genotyping by sequencing), as well as by additional analyses of three chloroplast (cp) regions (trnL‐F, ndhF‐rpl32, and rpl32‐trnL) in 25 individuals of P. hakusanensis and 53 individuals of P. asiatica var. densiuscula covering the species' geographical distribution. Monophyly of P. hakusanensis was suggested by the nuclear marker analyses, whereas the cp haplotypes of P. hakusanensis were shared with P. asiatica var. densiuscula and P. asiatica in China. The disparity between the nuclear and cp data may be explained by the introgression of the cp genome (cp capture). This research provides a phylogenetic tree showing the position of P. hakusanensis within subgenus Plantago and molecular evidence that implies complicated introgressions between P. hakusanensis and P. asiatica var. densiuscula. |
| format | Article |
| id | doaj-art-05a6905efc3b443fa3245ba0ac412a28 |
| institution | DOAJ |
| issn | 2045-7758 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj-art-05a6905efc3b443fa3245ba0ac412a282025-08-20T03:14:22ZengWileyEcology and Evolution2045-77582025-03-01153n/an/a10.1002/ece3.71144Allotetraploid Origin and Putative Ancient Introgression in Plantago hakusanensis (Plantaginaceae)Naoko Ishikawa0Shota Sakaguchi1Chikako Hasekura2Alexey Shipunov3Ayumi Matsuo4Yoshihisa Suyama5Hirokazu Tsukaya6Hiroshi Ikeda7Motomi Ito8Kawatabi Field Science Center, Graduate School of Agricultural Science Tohoku University Osaki Miyagi JapanGraduate School of Human and Environmental Studies Kyoto University, Yoshida‐Nihonmatsu‐Cho Kyoto JapanFaculty of Agriculture Tokyo University of Agriculture Atsugi Kanagawa JapanDepartment of Biology Minot State University Minot North Dakota USAGENODAS Inc Urbannet Sendai‐Chuo Bld Sendai Miyagi JapanKawatabi Field Science Center, Graduate School of Agricultural Science Tohoku University Osaki Miyagi JapanGraduate School of Science The University of Tokyo Tokyo JapanThe University Museum The University of Tokyo Tokyo JapanGraduate School of Arts and Sciences the University of Tokyo Tokyo JapanABSTRACT Plantago hakusanensis (2n = 4x = 24) is an endangered endemic species that occurs in subalpine zones in Japan. To clarify the unresolved phylogenetic position of P. hakusanensis within subgenus Plantago, we constructed a phylogenetic tree based on the nuclear‐encoded single‐copy gene sucrose–proton symporter 1 (SUC1) using 60 previously reported alleles from 24 taxa in subgenus Plantago. We found that P. hakusanensis was closely related to Plantago asiatica var. densiuscula. The phylogenetic relationships between P. hakusanensis and P. asiatica var. densiuscula were further examined by analyses of the SUC1 nuclear regions and the internal transcribed spacer (ITS) of rDNA, genome‐wide single‐nucleotide polymorphism genotyping (via multiplexed inter‐simple sequence repeat genotyping by sequencing), as well as by additional analyses of three chloroplast (cp) regions (trnL‐F, ndhF‐rpl32, and rpl32‐trnL) in 25 individuals of P. hakusanensis and 53 individuals of P. asiatica var. densiuscula covering the species' geographical distribution. Monophyly of P. hakusanensis was suggested by the nuclear marker analyses, whereas the cp haplotypes of P. hakusanensis were shared with P. asiatica var. densiuscula and P. asiatica in China. The disparity between the nuclear and cp data may be explained by the introgression of the cp genome (cp capture). This research provides a phylogenetic tree showing the position of P. hakusanensis within subgenus Plantago and molecular evidence that implies complicated introgressions between P. hakusanensis and P. asiatica var. densiuscula.https://doi.org/10.1002/ece3.71144allopolyploidchloroplast captureendangered speciesintrogressionphylogeographical inferencePlantago |
| spellingShingle | Naoko Ishikawa Shota Sakaguchi Chikako Hasekura Alexey Shipunov Ayumi Matsuo Yoshihisa Suyama Hirokazu Tsukaya Hiroshi Ikeda Motomi Ito Allotetraploid Origin and Putative Ancient Introgression in Plantago hakusanensis (Plantaginaceae) Ecology and Evolution allopolyploid chloroplast capture endangered species introgression phylogeographical inference Plantago |
| title | Allotetraploid Origin and Putative Ancient Introgression in Plantago hakusanensis (Plantaginaceae) |
| title_full | Allotetraploid Origin and Putative Ancient Introgression in Plantago hakusanensis (Plantaginaceae) |
| title_fullStr | Allotetraploid Origin and Putative Ancient Introgression in Plantago hakusanensis (Plantaginaceae) |
| title_full_unstemmed | Allotetraploid Origin and Putative Ancient Introgression in Plantago hakusanensis (Plantaginaceae) |
| title_short | Allotetraploid Origin and Putative Ancient Introgression in Plantago hakusanensis (Plantaginaceae) |
| title_sort | allotetraploid origin and putative ancient introgression in plantago hakusanensis plantaginaceae |
| topic | allopolyploid chloroplast capture endangered species introgression phylogeographical inference Plantago |
| url | https://doi.org/10.1002/ece3.71144 |
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