Extracellular vesicles: From large-scale production and engineering to clinical applications

Extracellular vesicles (EVs) have emerged as a promising strategy for treating a wide spectrum of pathologies, as they can deliver their cargo to recipient cells and regulate the signaling pathway of these cells to modulate their fate. Despite the great potential of EVs in clinical applications, the...

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Main Authors: Yuxuan Wang, Jiali Xiong, Kun Ouyang, Mingwang Ling, Junyi Luo, Jiajie Sun, Qianyun Xi, Ting Chen, Yongliang Zhang
Format: Article
Language:English
Published: SAGE Publishing 2025-04-01
Series:Journal of Tissue Engineering
Online Access:https://doi.org/10.1177/20417314251319474
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author Yuxuan Wang
Jiali Xiong
Kun Ouyang
Mingwang Ling
Junyi Luo
Jiajie Sun
Qianyun Xi
Ting Chen
Yongliang Zhang
author_facet Yuxuan Wang
Jiali Xiong
Kun Ouyang
Mingwang Ling
Junyi Luo
Jiajie Sun
Qianyun Xi
Ting Chen
Yongliang Zhang
author_sort Yuxuan Wang
collection DOAJ
description Extracellular vesicles (EVs) have emerged as a promising strategy for treating a wide spectrum of pathologies, as they can deliver their cargo to recipient cells and regulate the signaling pathway of these cells to modulate their fate. Despite the great potential of EVs in clinical applications, their low yield and the challenges of cargo loading remain significant obstacles, hindering their transition from experimental research to clinical practice. Therefore, promoting EV release and enhancing EV cargo-loading are promising fields with substantial research potential and broad application prospects. In this review, we summarize the clinical applications of EVs, the methods and technologies for their large-scale production, engineering, and modification, as well as the challenges that must be addressed during their development. We also discuss the future perspectives of this exciting field of research to facilitate its transformation from bench to clinical reality.
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id doaj-art-059cd76edcbb403890e6ad483c84f99b
institution DOAJ
issn 2041-7314
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publishDate 2025-04-01
publisher SAGE Publishing
record_format Article
series Journal of Tissue Engineering
spelling doaj-art-059cd76edcbb403890e6ad483c84f99b2025-08-20T02:55:52ZengSAGE PublishingJournal of Tissue Engineering2041-73142025-04-011610.1177/20417314251319474Extracellular vesicles: From large-scale production and engineering to clinical applicationsYuxuan Wang0Jiali Xiong1Kun Ouyang2Mingwang Ling3Junyi Luo4Jiajie Sun5Qianyun Xi6Ting Chen7Yongliang Zhang8College of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, Guangdong, ChinaCollege of Medicine, Jiaxing University, Jiaxing, Zhejiang, ChinaCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, Guangdong, ChinaCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, Guangdong, ChinaCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, Guangdong, ChinaCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, Guangdong, ChinaCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, Guangdong, ChinaCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, Guangdong, ChinaCollege of Animal Science, Guangdong Province Key Laboratory of Animal Nutritional Regulation, National Engineering Research Center for Breeding Swine Industry, State Key Laboratory of Livestock and Poultry Breeding, South China Agricultural University, Guangzhou, Guangdong, ChinaExtracellular vesicles (EVs) have emerged as a promising strategy for treating a wide spectrum of pathologies, as they can deliver their cargo to recipient cells and regulate the signaling pathway of these cells to modulate their fate. Despite the great potential of EVs in clinical applications, their low yield and the challenges of cargo loading remain significant obstacles, hindering their transition from experimental research to clinical practice. Therefore, promoting EV release and enhancing EV cargo-loading are promising fields with substantial research potential and broad application prospects. In this review, we summarize the clinical applications of EVs, the methods and technologies for their large-scale production, engineering, and modification, as well as the challenges that must be addressed during their development. We also discuss the future perspectives of this exciting field of research to facilitate its transformation from bench to clinical reality.https://doi.org/10.1177/20417314251319474
spellingShingle Yuxuan Wang
Jiali Xiong
Kun Ouyang
Mingwang Ling
Junyi Luo
Jiajie Sun
Qianyun Xi
Ting Chen
Yongliang Zhang
Extracellular vesicles: From large-scale production and engineering to clinical applications
Journal of Tissue Engineering
title Extracellular vesicles: From large-scale production and engineering to clinical applications
title_full Extracellular vesicles: From large-scale production and engineering to clinical applications
title_fullStr Extracellular vesicles: From large-scale production and engineering to clinical applications
title_full_unstemmed Extracellular vesicles: From large-scale production and engineering to clinical applications
title_short Extracellular vesicles: From large-scale production and engineering to clinical applications
title_sort extracellular vesicles from large scale production and engineering to clinical applications
url https://doi.org/10.1177/20417314251319474
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