A Novel Allelic Variant of <i>OsAGPL2</i> Influences Rice Eating and Cooking Quality
Starch biosynthesis is crucial in determining rice quality during rice endosperm development. This study obtained a stable inheritable white-core endosperm mutant, <i>h5</i>, by treating the japonica rice variety Nipponbare with MNU (N-methyl-N-nitro-sourea). The mutated gene is an allel...
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MDPI AG
2025-04-01
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| author | Yuqing Dan Fudeng Huang Junfeng Xu Yong He Ruixiao Peng Chunshou Li Jiayu Song Yuanyuan Hao Zhihong Tian |
| author_facet | Yuqing Dan Fudeng Huang Junfeng Xu Yong He Ruixiao Peng Chunshou Li Jiayu Song Yuanyuan Hao Zhihong Tian |
| author_sort | Yuqing Dan |
| collection | DOAJ |
| description | Starch biosynthesis is crucial in determining rice quality during rice endosperm development. This study obtained a stable inheritable white-core endosperm mutant, <i>h5</i>, by treating the japonica rice variety Nipponbare with MNU (N-methyl-N-nitro-sourea). The mutated gene is an allele of <i>OsAGPL2</i>, which encodes the large subunit of ADP-glucose pyrophosphorylase (AGPase), a key and rate-limiting enzyme in the rice starch biosynthesis pathway. A G-C mutation in the third exon of <i>OsAGPL2</i> led to impaired starch synthesis, significantly reduced amylose content (AC) and gel consistency (GC), and a marked decrease in AGPase activity. The haplotype analysis revealed that an SNP in the 3′UTR and two SNPs in the 5′UTR of <i>OsAGPL2</i> were associated with significant differences in AC and GC among rice resources. These SNPs can be utilized to design molecular markers for breeding programs to improve rice quality. This study elucidates the impact of OsAGPL2 on the eating and cooking quality of rice. It identifies superior haplotypes, providing a theoretical foundation and molecular markers for accumulating minor-effect genes to enhance rice quality. |
| format | Article |
| id | doaj-art-059ca43d93ba43bdb2e5dda2823be876 |
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| issn | 2073-4409 |
| language | English |
| publishDate | 2025-04-01 |
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| series | Cells |
| spelling | doaj-art-059ca43d93ba43bdb2e5dda2823be8762025-08-20T02:24:47ZengMDPI AGCells2073-44092025-04-0114963410.3390/cells14090634A Novel Allelic Variant of <i>OsAGPL2</i> Influences Rice Eating and Cooking QualityYuqing Dan0Fudeng Huang1Junfeng Xu2Yong He3Ruixiao Peng4Chunshou Li5Jiayu Song6Yuanyuan Hao7Zhihong Tian8Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, College of Life Science, Yangtze University, Jingzhou 434025, ChinaInstitute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaEngineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, College of Life Science, Yangtze University, Jingzhou 434025, ChinaEngineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, College of Life Science, Yangtze University, Jingzhou 434025, ChinaInstitute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaEngineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, College of Life Science, Yangtze University, Jingzhou 434025, ChinaStarch biosynthesis is crucial in determining rice quality during rice endosperm development. This study obtained a stable inheritable white-core endosperm mutant, <i>h5</i>, by treating the japonica rice variety Nipponbare with MNU (N-methyl-N-nitro-sourea). The mutated gene is an allele of <i>OsAGPL2</i>, which encodes the large subunit of ADP-glucose pyrophosphorylase (AGPase), a key and rate-limiting enzyme in the rice starch biosynthesis pathway. A G-C mutation in the third exon of <i>OsAGPL2</i> led to impaired starch synthesis, significantly reduced amylose content (AC) and gel consistency (GC), and a marked decrease in AGPase activity. The haplotype analysis revealed that an SNP in the 3′UTR and two SNPs in the 5′UTR of <i>OsAGPL2</i> were associated with significant differences in AC and GC among rice resources. These SNPs can be utilized to design molecular markers for breeding programs to improve rice quality. This study elucidates the impact of OsAGPL2 on the eating and cooking quality of rice. It identifies superior haplotypes, providing a theoretical foundation and molecular markers for accumulating minor-effect genes to enhance rice quality.https://www.mdpi.com/2073-4409/14/9/634rice quality<i>OsAGPL2</i>white-core endosperm mutantsuperior haplotype |
| spellingShingle | Yuqing Dan Fudeng Huang Junfeng Xu Yong He Ruixiao Peng Chunshou Li Jiayu Song Yuanyuan Hao Zhihong Tian A Novel Allelic Variant of <i>OsAGPL2</i> Influences Rice Eating and Cooking Quality Cells rice quality <i>OsAGPL2</i> white-core endosperm mutant superior haplotype |
| title | A Novel Allelic Variant of <i>OsAGPL2</i> Influences Rice Eating and Cooking Quality |
| title_full | A Novel Allelic Variant of <i>OsAGPL2</i> Influences Rice Eating and Cooking Quality |
| title_fullStr | A Novel Allelic Variant of <i>OsAGPL2</i> Influences Rice Eating and Cooking Quality |
| title_full_unstemmed | A Novel Allelic Variant of <i>OsAGPL2</i> Influences Rice Eating and Cooking Quality |
| title_short | A Novel Allelic Variant of <i>OsAGPL2</i> Influences Rice Eating and Cooking Quality |
| title_sort | novel allelic variant of i osagpl2 i influences rice eating and cooking quality |
| topic | rice quality <i>OsAGPL2</i> white-core endosperm mutant superior haplotype |
| url | https://www.mdpi.com/2073-4409/14/9/634 |
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