Targeting the PI3K-AKT pathway to alleviate 6PPDQ-induced cognitive impairment associated with metabolic syndrome

6PPD-quinone (6PPDQ), a derivative of the antioxidant 6PPD used in the rubber industry, has been implicated in environmental and public health risks. To explore its impact on metabolic and cognitive functions, we orally administered 6PPDQ to mice and assessed its effects. Our results show that 6PPDQ...

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Main Authors: Leisheng Wang, Yifei Fu, Shiwei Xu, Enhong Chen, Shuo Zhang, Dongjie Yang, Youzhao He, Yuanlong Gu, Yong Mao, Fenglai Yuan, Hao Hu
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Ecotoxicology and Environmental Safety
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Online Access:http://www.sciencedirect.com/science/article/pii/S0147651325010565
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author Leisheng Wang
Yifei Fu
Shiwei Xu
Enhong Chen
Shuo Zhang
Dongjie Yang
Youzhao He
Yuanlong Gu
Yong Mao
Fenglai Yuan
Hao Hu
author_facet Leisheng Wang
Yifei Fu
Shiwei Xu
Enhong Chen
Shuo Zhang
Dongjie Yang
Youzhao He
Yuanlong Gu
Yong Mao
Fenglai Yuan
Hao Hu
author_sort Leisheng Wang
collection DOAJ
description 6PPD-quinone (6PPDQ), a derivative of the antioxidant 6PPD used in the rubber industry, has been implicated in environmental and public health risks. To explore its impact on metabolic and cognitive functions, we orally administered 6PPDQ to mice and assessed its effects. Our results show that 6PPDQ induces a dose-dependent increase in body weight, elevated fasting blood glucose, insulin resistance, and other hallmarks of metabolic syndrome. Furthermore, cognitive tests revealed significant impairments in learning and memory in 6PPDQ-treated mice. Transcriptomic and Western blot analyses suggest that 6PPDQ disrupts insulin signaling through inhibition of the PI3K-AKT pathway, leading to metabolic and cognitive dysfunction. Notably, treatment with the PI3K-AKT agonist SC79 partially alleviated these effects. These findings highlight the health risks of 6PPDQ exposure and suggest potential avenues for preventive and therapeutic strategies.
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institution Kabale University
issn 0147-6513
language English
publishDate 2025-09-01
publisher Elsevier
record_format Article
series Ecotoxicology and Environmental Safety
spelling doaj-art-05906009065a4e53bb8b4c9d82e968672025-08-20T03:41:53ZengElsevierEcotoxicology and Environmental Safety0147-65132025-09-0130211871110.1016/j.ecoenv.2025.118711Targeting the PI3K-AKT pathway to alleviate 6PPDQ-induced cognitive impairment associated with metabolic syndromeLeisheng Wang0Yifei Fu1Shiwei Xu2Enhong Chen3Shuo Zhang4Dongjie Yang5Youzhao He6Yuanlong Gu7Yong Mao8Fenglai Yuan9Hao Hu10Affiliated Hospital of Jiangnan University, Wuxi 214122, China; Wuxi Medical College, Jiangnan University, Wuxi 214122, China; Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, ChinaAffiliated Hospital of Jiangnan University, Wuxi 214122, China; Wuxi Medical College, Jiangnan University, Wuxi 214122, ChinaAffiliated Hospital of Jiangnan University, Wuxi 214122, China; Wuxi Medical College, Jiangnan University, Wuxi 214122, ChinaAffiliated Hospital of Jiangnan University, Wuxi 214122, ChinaAffiliated Hospital of Jiangnan University, Wuxi 214122, ChinaAffiliated Hospital of Jiangnan University, Wuxi 214122, ChinaAffiliated Hospital of Jiangnan University, Wuxi 214122, ChinaAffiliated Hospital of Jiangnan University, Wuxi 214122, ChinaAffiliated Hospital of Jiangnan University, Wuxi 214122, China; Wuxi Medical College, Jiangnan University, Wuxi 214122, China; Corresponding authors at: Affiliated Hospital of Jiangnan University, Wuxi 214122, China.Affiliated Hospital of Jiangnan University, Wuxi 214122, China; Wuxi Medical College, Jiangnan University, Wuxi 214122, China; Corresponding authors at: Affiliated Hospital of Jiangnan University, Wuxi 214122, China.Affiliated Hospital of Jiangnan University, Wuxi 214122, China; Wuxi Medical College, Jiangnan University, Wuxi 214122, China; Corresponding authors at: Affiliated Hospital of Jiangnan University, Wuxi 214122, China.6PPD-quinone (6PPDQ), a derivative of the antioxidant 6PPD used in the rubber industry, has been implicated in environmental and public health risks. To explore its impact on metabolic and cognitive functions, we orally administered 6PPDQ to mice and assessed its effects. Our results show that 6PPDQ induces a dose-dependent increase in body weight, elevated fasting blood glucose, insulin resistance, and other hallmarks of metabolic syndrome. Furthermore, cognitive tests revealed significant impairments in learning and memory in 6PPDQ-treated mice. Transcriptomic and Western blot analyses suggest that 6PPDQ disrupts insulin signaling through inhibition of the PI3K-AKT pathway, leading to metabolic and cognitive dysfunction. Notably, treatment with the PI3K-AKT agonist SC79 partially alleviated these effects. These findings highlight the health risks of 6PPDQ exposure and suggest potential avenues for preventive and therapeutic strategies.http://www.sciencedirect.com/science/article/pii/S01476513250105656PPD-quinoneMetabolic syndromeCognitive impairment
spellingShingle Leisheng Wang
Yifei Fu
Shiwei Xu
Enhong Chen
Shuo Zhang
Dongjie Yang
Youzhao He
Yuanlong Gu
Yong Mao
Fenglai Yuan
Hao Hu
Targeting the PI3K-AKT pathway to alleviate 6PPDQ-induced cognitive impairment associated with metabolic syndrome
Ecotoxicology and Environmental Safety
6PPD-quinone
Metabolic syndrome
Cognitive impairment
title Targeting the PI3K-AKT pathway to alleviate 6PPDQ-induced cognitive impairment associated with metabolic syndrome
title_full Targeting the PI3K-AKT pathway to alleviate 6PPDQ-induced cognitive impairment associated with metabolic syndrome
title_fullStr Targeting the PI3K-AKT pathway to alleviate 6PPDQ-induced cognitive impairment associated with metabolic syndrome
title_full_unstemmed Targeting the PI3K-AKT pathway to alleviate 6PPDQ-induced cognitive impairment associated with metabolic syndrome
title_short Targeting the PI3K-AKT pathway to alleviate 6PPDQ-induced cognitive impairment associated with metabolic syndrome
title_sort targeting the pi3k akt pathway to alleviate 6ppdq induced cognitive impairment associated with metabolic syndrome
topic 6PPD-quinone
Metabolic syndrome
Cognitive impairment
url http://www.sciencedirect.com/science/article/pii/S0147651325010565
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