Imaging Plant Lipids with Fluorescent Reporters
In plants, lipids function as structural elements and signaling molecules. Understanding lipid composition and dynamics is essential for unraveling their biological functions and metabolism. Mapping the spatiotemporal distribution of lipids in plants holds great potential for elucidating lipid biosy...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/14/1/15 |
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| author | Yong-Kang Li Guang-Yi Dai Yu-Meng Zhang Nan Yao |
| author_facet | Yong-Kang Li Guang-Yi Dai Yu-Meng Zhang Nan Yao |
| author_sort | Yong-Kang Li |
| collection | DOAJ |
| description | In plants, lipids function as structural elements and signaling molecules. Understanding lipid composition and dynamics is essential for unraveling their biological functions and metabolism. Mapping the spatiotemporal distribution of lipids in plants holds great potential for elucidating lipid biosynthetic pathways and gaining insights to guide crop genetic engineering. Recent progress in fluorescence microscopy and imaging has opened new opportunities for researchers to visualize plant lipids <i>in vivo</i> at high spatiotemporal resolution. In this review, we provide an up-to-date overview of the methods used to image plant lipids with fluorescence microscopy. We highlight caveats and potential limitations of these approaches and provide suggestions for optimizing their utilization. This review synthesizes current knowledge and highlights the potential of these methods to provide new insights into lipid biology. |
| format | Article |
| id | doaj-art-058f8aa205b14e0aa0918bb8e14a7248 |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-058f8aa205b14e0aa0918bb8e14a72482025-01-10T13:19:30ZengMDPI AGPlants2223-77472024-12-011411510.3390/plants14010015Imaging Plant Lipids with Fluorescent ReportersYong-Kang Li0Guang-Yi Dai1Yu-Meng Zhang2Nan Yao3Guangdong Provincial Key Laboratory of Plant Stress Biology, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaSouth China National Botanical Garden, Chinese Academy of Sciences, Guangzhou 510275, ChinaGuangdong Provincial Key Laboratory of Plant Stress Biology, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaGuangdong Provincial Key Laboratory of Plant Stress Biology, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaIn plants, lipids function as structural elements and signaling molecules. Understanding lipid composition and dynamics is essential for unraveling their biological functions and metabolism. Mapping the spatiotemporal distribution of lipids in plants holds great potential for elucidating lipid biosynthetic pathways and gaining insights to guide crop genetic engineering. Recent progress in fluorescence microscopy and imaging has opened new opportunities for researchers to visualize plant lipids <i>in vivo</i> at high spatiotemporal resolution. In this review, we provide an up-to-date overview of the methods used to image plant lipids with fluorescence microscopy. We highlight caveats and potential limitations of these approaches and provide suggestions for optimizing their utilization. This review synthesizes current knowledge and highlights the potential of these methods to provide new insights into lipid biology.https://www.mdpi.com/2223-7747/14/1/15plant lipidsvisualizationfluorescencebiosensorspatiotemporal dynamics |
| spellingShingle | Yong-Kang Li Guang-Yi Dai Yu-Meng Zhang Nan Yao Imaging Plant Lipids with Fluorescent Reporters Plants plant lipids visualization fluorescence biosensor spatiotemporal dynamics |
| title | Imaging Plant Lipids with Fluorescent Reporters |
| title_full | Imaging Plant Lipids with Fluorescent Reporters |
| title_fullStr | Imaging Plant Lipids with Fluorescent Reporters |
| title_full_unstemmed | Imaging Plant Lipids with Fluorescent Reporters |
| title_short | Imaging Plant Lipids with Fluorescent Reporters |
| title_sort | imaging plant lipids with fluorescent reporters |
| topic | plant lipids visualization fluorescence biosensor spatiotemporal dynamics |
| url | https://www.mdpi.com/2223-7747/14/1/15 |
| work_keys_str_mv | AT yongkangli imagingplantlipidswithfluorescentreporters AT guangyidai imagingplantlipidswithfluorescentreporters AT yumengzhang imagingplantlipidswithfluorescentreporters AT nanyao imagingplantlipidswithfluorescentreporters |