Effects of Adding Punicalagin or Oleuropein to TRIS Diluent on Quality of Frozen–Thawed Semen from Rams
This study aimed to investigate the effects of adding different levels of punicalagin or oleuropein to TRIS diluent on the quality of frozen–thawed semen from Najdi rams. Semen was diluted using TRIS-based diluter with 15% egg yolk (control group); supplemented with 0.1, 0.5, or 1 mg/100 mL punicala...
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MDPI AG
2025-04-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/15/9/1242 |
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| author | Mohamed Shehab-El-Deen Mohamed Ali Ibrahim Alolayan Abdullah Aljuaythin Yasser Alrauji Soliman Aldobaib Shaaban S. Elnesr |
| author_facet | Mohamed Shehab-El-Deen Mohamed Ali Ibrahim Alolayan Abdullah Aljuaythin Yasser Alrauji Soliman Aldobaib Shaaban S. Elnesr |
| author_sort | Mohamed Shehab-El-Deen |
| collection | DOAJ |
| description | This study aimed to investigate the effects of adding different levels of punicalagin or oleuropein to TRIS diluent on the quality of frozen–thawed semen from Najdi rams. Semen was diluted using TRIS-based diluter with 15% egg yolk (control group); supplemented with 0.1, 0.5, or 1 mg/100 mL punicalagin (in Experiment 1); or supplemented with 1, 2.5, or 5 mg/100 mL oleuropein (in Experiment 2). The collected semen was evaluated and cryopreserved, with the motility and concentration of sperm assessed using a CASA system. The results showed that the total motile spermatozoa (TMS), percentage of progressive motile spermatozoa (PMS), curvilinear velocity (VCL), rectilinear velocity, average path velocity (VAP), linearity coefficient, straightness index, minor defects, and sperm vitality were higher in the 0.1 mg/100 mL punicalagin group (<i>p</i> < 0.05) than in other groups. HOST% post-thawing was significantly higher in all punicalagin groups compared to the control group (<i>p</i> < 0.001). The percentages of PMS, TMS, VCL, minor defects, and sperm vitality were higher in the 1 mg/100 mL oleuropein group (<i>p</i> < 0.05) than in other groups. Oleuropein supplementation at 5 mg/100 mL decreased VAP in cooled sperms, while all levels increased VAP post-thawing. HOST-positive sperms% post-thawing was higher in all oleuropein-treated groups than the control group (<i>p</i> < 0.001). Moreover, oleuropein nonsignificantly increased the acrosome integrity in cooled sperms, while higher studied concentrations of oleuropein (2.5 and 5 mg/100 mL) decreased the acrosome integrity in frozen sperms. In conclusion, adding punicalagin (0.1 mg/100 mL) or oleuropein (1 mg/100 mL) to TRIS diluent improved the quality of frozen–thawed semen from rams. |
| format | Article |
| id | doaj-art-0581efbb56244c3c8f2613dda2e216d2 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-0581efbb56244c3c8f2613dda2e216d22025-08-20T03:52:57ZengMDPI AGAnimals2076-26152025-04-01159124210.3390/ani15091242Effects of Adding Punicalagin or Oleuropein to TRIS Diluent on Quality of Frozen–Thawed Semen from RamsMohamed Shehab-El-Deen0Mohamed Ali1Ibrahim Alolayan2Abdullah Aljuaythin3Yasser Alrauji4Soliman Aldobaib5Shaaban S. Elnesr6Department of Animal and Poultry Production, College of Agriculture and Food, Qassim University, Buraydah 51452, Al-Qassim, Saudi ArabiaDepartment of Animal and Poultry Production, College of Agriculture and Food, Qassim University, Buraydah 51452, Al-Qassim, Saudi ArabiaDepartment of Animal and Poultry Production, College of Agriculture and Food, Qassim University, Buraydah 51452, Al-Qassim, Saudi ArabiaDepartment of Animal and Poultry Production, College of Agriculture and Food, Qassim University, Buraydah 51452, Al-Qassim, Saudi ArabiaDepartment of Animal and Poultry Production, College of Agriculture and Food, Qassim University, Buraydah 51452, Al-Qassim, Saudi ArabiaDepartment of Animal and Poultry Production, College of Agriculture and Food, Qassim University, Buraydah 51452, Al-Qassim, Saudi ArabiaDepartment of Poultry Production, Faculty of Agriculture, Fayoum University, Fayoum 63514, EgyptThis study aimed to investigate the effects of adding different levels of punicalagin or oleuropein to TRIS diluent on the quality of frozen–thawed semen from Najdi rams. Semen was diluted using TRIS-based diluter with 15% egg yolk (control group); supplemented with 0.1, 0.5, or 1 mg/100 mL punicalagin (in Experiment 1); or supplemented with 1, 2.5, or 5 mg/100 mL oleuropein (in Experiment 2). The collected semen was evaluated and cryopreserved, with the motility and concentration of sperm assessed using a CASA system. The results showed that the total motile spermatozoa (TMS), percentage of progressive motile spermatozoa (PMS), curvilinear velocity (VCL), rectilinear velocity, average path velocity (VAP), linearity coefficient, straightness index, minor defects, and sperm vitality were higher in the 0.1 mg/100 mL punicalagin group (<i>p</i> < 0.05) than in other groups. HOST% post-thawing was significantly higher in all punicalagin groups compared to the control group (<i>p</i> < 0.001). The percentages of PMS, TMS, VCL, minor defects, and sperm vitality were higher in the 1 mg/100 mL oleuropein group (<i>p</i> < 0.05) than in other groups. Oleuropein supplementation at 5 mg/100 mL decreased VAP in cooled sperms, while all levels increased VAP post-thawing. HOST-positive sperms% post-thawing was higher in all oleuropein-treated groups than the control group (<i>p</i> < 0.001). Moreover, oleuropein nonsignificantly increased the acrosome integrity in cooled sperms, while higher studied concentrations of oleuropein (2.5 and 5 mg/100 mL) decreased the acrosome integrity in frozen sperms. In conclusion, adding punicalagin (0.1 mg/100 mL) or oleuropein (1 mg/100 mL) to TRIS diluent improved the quality of frozen–thawed semen from rams.https://www.mdpi.com/2076-2615/15/9/1242sheepsemen cryopreservationextenderantioxidantspunicalaginoleuropein |
| spellingShingle | Mohamed Shehab-El-Deen Mohamed Ali Ibrahim Alolayan Abdullah Aljuaythin Yasser Alrauji Soliman Aldobaib Shaaban S. Elnesr Effects of Adding Punicalagin or Oleuropein to TRIS Diluent on Quality of Frozen–Thawed Semen from Rams Animals sheep semen cryopreservation extender antioxidants punicalagin oleuropein |
| title | Effects of Adding Punicalagin or Oleuropein to TRIS Diluent on Quality of Frozen–Thawed Semen from Rams |
| title_full | Effects of Adding Punicalagin or Oleuropein to TRIS Diluent on Quality of Frozen–Thawed Semen from Rams |
| title_fullStr | Effects of Adding Punicalagin or Oleuropein to TRIS Diluent on Quality of Frozen–Thawed Semen from Rams |
| title_full_unstemmed | Effects of Adding Punicalagin or Oleuropein to TRIS Diluent on Quality of Frozen–Thawed Semen from Rams |
| title_short | Effects of Adding Punicalagin or Oleuropein to TRIS Diluent on Quality of Frozen–Thawed Semen from Rams |
| title_sort | effects of adding punicalagin or oleuropein to tris diluent on quality of frozen thawed semen from rams |
| topic | sheep semen cryopreservation extender antioxidants punicalagin oleuropein |
| url | https://www.mdpi.com/2076-2615/15/9/1242 |
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