Genome-wide identification and bioinformatics analysis of PRX gene family in Brassica rapa

In order to further study the functions of class Ⅲ peroxidase (PRX) family genes in Brassica rapa (BrPRX), the BrPRX was identified by genome-wide analysis, and its bioinformatics analysis was carried out. The results showed that there were a total of 121 BrPRX members, contained 0-9 introns, exhibi...

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Main Authors: CHEN Guohu, WANG Hao, LI Guang, TANG Xiaoyan, WANG Chenggang, ZHANG Lei, HOU Jinfeng, YUAN Lingyun
Format: Article
Language:English
Published: Zhejiang University Press 2020-12-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2020.06.011
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author CHEN Guohu
WANG Hao
LI Guang
TANG Xiaoyan
WANG Chenggang
ZHANG Lei
HOU Jinfeng
YUAN Lingyun
author_facet CHEN Guohu
WANG Hao
LI Guang
TANG Xiaoyan
WANG Chenggang
ZHANG Lei
HOU Jinfeng
YUAN Lingyun
author_sort CHEN Guohu
collection DOAJ
description In order to further study the functions of class Ⅲ peroxidase (PRX) family genes in Brassica rapa (BrPRX), the BrPRX was identified by genome-wide analysis, and its bioinformatics analysis was carried out. The results showed that there were a total of 121 BrPRX members, contained 0-9 introns, exhibited unequal distribution on chromosomes, and multiple pairs of BrPRX genes were distributed on chromosomes with tandem duplication. Phylogenetic analysis of PRX family genes from Arabidopsis thaliana and B. rapa suggested that these members could be divided into five groups. Synteny and K<sub>a</sub>/K<sub>s</sub> analyses demonstrated that B. rapa had a high homology with A. thaliana, and most of BrPRX family gene had undergone strong purifying selection in the evolution. The analysis of cis-acting regulatory elements of BrPRX family genes showed that they played important roles in hormone response regulation, light response and resistance to adversity. In addition, transcriptome data analysis showed that the BrPRX genes had tissue expression specificity, and some of them were down-regulated in the male sterility materials. These results lay a foundation for the further study of PRX genes’ function in B. rapa.
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series 浙江大学学报. 农业与生命科学版
spelling doaj-art-0569a7b4d4ee48819b5848a10aa7eeaf2025-08-20T03:16:01ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552020-12-014667768610.3785/j.issn.1008-9209.2020.06.01110089209Genome-wide identification and bioinformatics analysis of PRX gene family in Brassica rapaCHEN GuohuWANG HaoLI GuangTANG XiaoyanWANG ChenggangZHANG LeiHOU JinfengYUAN LingyunIn order to further study the functions of class Ⅲ peroxidase (PRX) family genes in Brassica rapa (BrPRX), the BrPRX was identified by genome-wide analysis, and its bioinformatics analysis was carried out. The results showed that there were a total of 121 BrPRX members, contained 0-9 introns, exhibited unequal distribution on chromosomes, and multiple pairs of BrPRX genes were distributed on chromosomes with tandem duplication. Phylogenetic analysis of PRX family genes from Arabidopsis thaliana and B. rapa suggested that these members could be divided into five groups. Synteny and K<sub>a</sub>/K<sub>s</sub> analyses demonstrated that B. rapa had a high homology with A. thaliana, and most of BrPRX family gene had undergone strong purifying selection in the evolution. The analysis of cis-acting regulatory elements of BrPRX family genes showed that they played important roles in hormone response regulation, light response and resistance to adversity. In addition, transcriptome data analysis showed that the BrPRX genes had tissue expression specificity, and some of them were down-regulated in the male sterility materials. These results lay a foundation for the further study of PRX genes’ function in B. rapa.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2020.06.011<italic>Brassica rapa</italic> peroxidasegenome-wide identificationtranscriptome analysis
spellingShingle CHEN Guohu
WANG Hao
LI Guang
TANG Xiaoyan
WANG Chenggang
ZHANG Lei
HOU Jinfeng
YUAN Lingyun
Genome-wide identification and bioinformatics analysis of PRX gene family in Brassica rapa
浙江大学学报. 农业与生命科学版
<italic>Brassica rapa</italic>
peroxidase
genome-wide identification
transcriptome analysis
title Genome-wide identification and bioinformatics analysis of PRX gene family in Brassica rapa
title_full Genome-wide identification and bioinformatics analysis of PRX gene family in Brassica rapa
title_fullStr Genome-wide identification and bioinformatics analysis of PRX gene family in Brassica rapa
title_full_unstemmed Genome-wide identification and bioinformatics analysis of PRX gene family in Brassica rapa
title_short Genome-wide identification and bioinformatics analysis of PRX gene family in Brassica rapa
title_sort genome wide identification and bioinformatics analysis of prx gene family in brassica rapa
topic <italic>Brassica rapa</italic>
peroxidase
genome-wide identification
transcriptome analysis
url https://www.academax.com/doi/10.3785/j.issn.1008-9209.2020.06.011
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