Retrospective evaluation of multiplex PCR panel results from CSF samples in a university hospital
Aim: Given the significant mortality and sequelae due to meningitis, rapid diagnosis and initiation of treatment have a major impact on patient outcomes. In many cases of meningitis/meningoencephalitis, empirical treatment should be initiated immediately. This empirical treatment regimen is initiat...
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| Format: | Article |
| Language: | English |
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Izzet Baysal Training and Research Hospital
2025-01-01
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| Series: | Northwestern Medical Journal |
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| Online Access: | https://nwmedj.org/article/view/90 |
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| author | Funda Şahin Nida Özcan Erdal Özbek Selahattin Atmaca Hakan Temiz |
| author_facet | Funda Şahin Nida Özcan Erdal Özbek Selahattin Atmaca Hakan Temiz |
| author_sort | Funda Şahin |
| collection | DOAJ |
| description |
Aim: Given the significant mortality and sequelae due to meningitis, rapid diagnosis and initiation of treatment have a major impact on patient outcomes. In many cases of meningitis/meningoencephalitis, empirical treatment should be initiated immediately. This empirical treatment regimen is initiated based on the cumulative antibiotic susceptibility results in the region. The aim of our study was to retrospectively determine the causative agents in cerebrospinal fluid samples of patients who received a presumptive diagnosis of meningitis, using Multiplex Polymerase Chain Reaction (PCR) tests.
Materials and Methods: The study included 206 cerebrospinal fluid samples from different patients with a preliminary diagnosis of meningitis sent from various clinics. The Biospeedy viral nucleic acid isolation kit (Bioeksen, Türkiye) was used for the isolation of genetic material. Genetic materials (DNA/RNA) related to Herpes simplex virus 1-2, Humman herpesvirus 6-7-8, Varicella zoster virus, Enterovirus, Cytomegalovirus, Human Parechoviruses, Haemophilus influenzae, Listeria monocytogenes, Streptococcus pneumoniae, Neisseria meningitidis, Streptococcus agalactiae, Escherichia coli K1, Cryptococcus gattii/neoformans in cerebrospinal fluid samples were investigated using the Meningitis/Encephalitis RT-qPCR MX-17 Panel (RT-qPCR MX-17S Panel, Bio-Speedy®, Bioeksen, Türkiye) multiplex PCR kit.
Results: According to the PCR results, the causative agent was identified in a total of 19 patients. Nine patients were found to have Streptococcus pneumoniae, two had Varicella zoster virus, and two had Enterovirus. Additionally, six patients had separate detections of Haemophilus influenzae, Cytomegalovirus, Herpes simplex virus 1, Human herpesvirus 6, Human herpesvirus 8, and Parechoviruses.
Conclusion: Recently, simple and rapid molecular tests such as PCR have contributed to an increase in the early detection of causative agents. Based on the performance of diagnostic tests, we propose an algorithm for the use of both syndromic and specific tests in patients at risk for meningitis/encephalitis.
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| format | Article |
| id | doaj-art-05567abb0f3c4e928ef01ddeac4001c6 |
| institution | Kabale University |
| issn | 2979-9538 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Izzet Baysal Training and Research Hospital |
| record_format | Article |
| series | Northwestern Medical Journal |
| spelling | doaj-art-05567abb0f3c4e928ef01ddeac4001c62025-02-04T12:17:08ZengIzzet Baysal Training and Research HospitalNorthwestern Medical Journal2979-95382025-01-015110.54307/2025.NWMJ.90Retrospective evaluation of multiplex PCR panel results from CSF samples in a university hospitalFunda Şahin0https://orcid.org/0000-0001-8406-2011Nida Özcan1https://orcid.org/0000-0001-6898-7516Erdal Özbek2https://orcid.org/0000-0002-8593-224XSelahattin Atmaca3https://orcid.org/0000-0002-2730-5790Hakan Temiz4https://orcid.org/0000-0002-3402-2625Department of Medical Microbiology, Faculty of Medicine, Adıyaman University, Adıyaman, TürkiyeDepartment of Medical Microbiology, Faculty of Medicine, Dicle University, Diyarbakır, TürkiyeDepartment of Medical Microbiology, Faculty of Medicine, Dicle University, Diyarbakır, TürkiyeDepartment of Medical Microbiology, Faculty of Medicine, Dicle University, Diyarbakır, TürkiyeDepartment of Medical Microbiology, Faculty of Medicine, Dicle University, Diyarbakır, Türkiye Aim: Given the significant mortality and sequelae due to meningitis, rapid diagnosis and initiation of treatment have a major impact on patient outcomes. In many cases of meningitis/meningoencephalitis, empirical treatment should be initiated immediately. This empirical treatment regimen is initiated based on the cumulative antibiotic susceptibility results in the region. The aim of our study was to retrospectively determine the causative agents in cerebrospinal fluid samples of patients who received a presumptive diagnosis of meningitis, using Multiplex Polymerase Chain Reaction (PCR) tests. Materials and Methods: The study included 206 cerebrospinal fluid samples from different patients with a preliminary diagnosis of meningitis sent from various clinics. The Biospeedy viral nucleic acid isolation kit (Bioeksen, Türkiye) was used for the isolation of genetic material. Genetic materials (DNA/RNA) related to Herpes simplex virus 1-2, Humman herpesvirus 6-7-8, Varicella zoster virus, Enterovirus, Cytomegalovirus, Human Parechoviruses, Haemophilus influenzae, Listeria monocytogenes, Streptococcus pneumoniae, Neisseria meningitidis, Streptococcus agalactiae, Escherichia coli K1, Cryptococcus gattii/neoformans in cerebrospinal fluid samples were investigated using the Meningitis/Encephalitis RT-qPCR MX-17 Panel (RT-qPCR MX-17S Panel, Bio-Speedy®, Bioeksen, Türkiye) multiplex PCR kit. Results: According to the PCR results, the causative agent was identified in a total of 19 patients. Nine patients were found to have Streptococcus pneumoniae, two had Varicella zoster virus, and two had Enterovirus. Additionally, six patients had separate detections of Haemophilus influenzae, Cytomegalovirus, Herpes simplex virus 1, Human herpesvirus 6, Human herpesvirus 8, and Parechoviruses. Conclusion: Recently, simple and rapid molecular tests such as PCR have contributed to an increase in the early detection of causative agents. Based on the performance of diagnostic tests, we propose an algorithm for the use of both syndromic and specific tests in patients at risk for meningitis/encephalitis. https://nwmedj.org/article/view/90cerebrospinal fluidmeningitismultiplex PCR |
| spellingShingle | Funda Şahin Nida Özcan Erdal Özbek Selahattin Atmaca Hakan Temiz Retrospective evaluation of multiplex PCR panel results from CSF samples in a university hospital Northwestern Medical Journal cerebrospinal fluid meningitis multiplex PCR |
| title | Retrospective evaluation of multiplex PCR panel results from CSF samples in a university hospital |
| title_full | Retrospective evaluation of multiplex PCR panel results from CSF samples in a university hospital |
| title_fullStr | Retrospective evaluation of multiplex PCR panel results from CSF samples in a university hospital |
| title_full_unstemmed | Retrospective evaluation of multiplex PCR panel results from CSF samples in a university hospital |
| title_short | Retrospective evaluation of multiplex PCR panel results from CSF samples in a university hospital |
| title_sort | retrospective evaluation of multiplex pcr panel results from csf samples in a university hospital |
| topic | cerebrospinal fluid meningitis multiplex PCR |
| url | https://nwmedj.org/article/view/90 |
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