Modified Nonnutrient Agar Plate Culture for the Diagnosis of Strongyloides stercoralis and Hookworm Infections in La-Ngu District, Satun Province, Southern Thailand
Due to the low prevalence and light intensity of Strongyloides stercoralis (S. stercoralis, Ss) and hookworm (HW) in Thailand, an increase in the efficacy of detection with the appropriate diagnosis is needed. This cross-sectional survey is aimed at using modified nonnutrient agar plate culture (mNN...
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Wiley
2022-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/1117400 |
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| author | Sirima Kitvatanachai Kanyanan Kritsiriwutthinan Aree Taylor Pochong Rhongbutsri |
| author_facet | Sirima Kitvatanachai Kanyanan Kritsiriwutthinan Aree Taylor Pochong Rhongbutsri |
| author_sort | Sirima Kitvatanachai |
| collection | DOAJ |
| description | Due to the low prevalence and light intensity of Strongyloides stercoralis (S. stercoralis, Ss) and hookworm (HW) in Thailand, an increase in the efficacy of detection with the appropriate diagnosis is needed. This cross-sectional survey is aimed at using modified nonnutrient agar plate culture (mNNAPC) to assess the prevalence of S. stercoralis and hookworm infections and to report intestinal parasitic infections in La-Ngu villagers, Satun Province in Southern Thailand. We used wet smear, modified Harada-Mori filter paper culture (mHMFPC), and mNNAPC to investigate 204 villagers (4 villages) between August 2016 and January 2017. The combination of mHMFPC and mNNAPC raised the prevalence of S. stercoralis and hookworm infections among the study population from 3.4% and 1.0% to 6.9% and 2.5%, respectively. There were no significant differences between demographic characteristics and these infections (p<0.05). Three types of protozoal infections, Blastocystis spp. (2.9%), Entamoeba histolytica-like (0.5%), and Giardia duodenalis (0.5%), and 3 species of helminthic infections, S. stercoralis (6.9%) (14 cases), hookworm 2.5%, and Enterobius vermicularis 0.5%, were demonstrated in this area. The mNNAPC showed the highest efficacy in detecting both parasites (Ss 92.9% and HW 80%), whereas the wet smear detected none. |
| format | Article |
| id | doaj-art-0543a2a456e149c8babc8a7032758538 |
| institution | OA Journals |
| issn | 2090-0031 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-0543a2a456e149c8babc8a70327585382025-08-20T02:02:59ZengWileyJournal of Parasitology Research2090-00312022-01-01202210.1155/2022/1117400Modified Nonnutrient Agar Plate Culture for the Diagnosis of Strongyloides stercoralis and Hookworm Infections in La-Ngu District, Satun Province, Southern ThailandSirima Kitvatanachai0Kanyanan Kritsiriwutthinan1Aree Taylor2Pochong Rhongbutsri3Faculty of Medical TechnologyFaculty of Medical TechnologyDepartment of Preclinical ScienceDepartment of Preclinical ScienceDue to the low prevalence and light intensity of Strongyloides stercoralis (S. stercoralis, Ss) and hookworm (HW) in Thailand, an increase in the efficacy of detection with the appropriate diagnosis is needed. This cross-sectional survey is aimed at using modified nonnutrient agar plate culture (mNNAPC) to assess the prevalence of S. stercoralis and hookworm infections and to report intestinal parasitic infections in La-Ngu villagers, Satun Province in Southern Thailand. We used wet smear, modified Harada-Mori filter paper culture (mHMFPC), and mNNAPC to investigate 204 villagers (4 villages) between August 2016 and January 2017. The combination of mHMFPC and mNNAPC raised the prevalence of S. stercoralis and hookworm infections among the study population from 3.4% and 1.0% to 6.9% and 2.5%, respectively. There were no significant differences between demographic characteristics and these infections (p<0.05). Three types of protozoal infections, Blastocystis spp. (2.9%), Entamoeba histolytica-like (0.5%), and Giardia duodenalis (0.5%), and 3 species of helminthic infections, S. stercoralis (6.9%) (14 cases), hookworm 2.5%, and Enterobius vermicularis 0.5%, were demonstrated in this area. The mNNAPC showed the highest efficacy in detecting both parasites (Ss 92.9% and HW 80%), whereas the wet smear detected none.http://dx.doi.org/10.1155/2022/1117400 |
| spellingShingle | Sirima Kitvatanachai Kanyanan Kritsiriwutthinan Aree Taylor Pochong Rhongbutsri Modified Nonnutrient Agar Plate Culture for the Diagnosis of Strongyloides stercoralis and Hookworm Infections in La-Ngu District, Satun Province, Southern Thailand Journal of Parasitology Research |
| title | Modified Nonnutrient Agar Plate Culture for the Diagnosis of Strongyloides stercoralis and Hookworm Infections in La-Ngu District, Satun Province, Southern Thailand |
| title_full | Modified Nonnutrient Agar Plate Culture for the Diagnosis of Strongyloides stercoralis and Hookworm Infections in La-Ngu District, Satun Province, Southern Thailand |
| title_fullStr | Modified Nonnutrient Agar Plate Culture for the Diagnosis of Strongyloides stercoralis and Hookworm Infections in La-Ngu District, Satun Province, Southern Thailand |
| title_full_unstemmed | Modified Nonnutrient Agar Plate Culture for the Diagnosis of Strongyloides stercoralis and Hookworm Infections in La-Ngu District, Satun Province, Southern Thailand |
| title_short | Modified Nonnutrient Agar Plate Culture for the Diagnosis of Strongyloides stercoralis and Hookworm Infections in La-Ngu District, Satun Province, Southern Thailand |
| title_sort | modified nonnutrient agar plate culture for the diagnosis of strongyloides stercoralis and hookworm infections in la ngu district satun province southern thailand |
| url | http://dx.doi.org/10.1155/2022/1117400 |
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