Tapak Liman (Elephantopus scaber L.) Leaves Ethanol Extract Improves the Production of IL-6 and IL-17 Cytokines in Mice with Bleomycin-induced Pulmonary Fibrosis
IL-6 and IL-17 are pro-inflammatory and pro-fibrotic cytokines that increase pulmonary fibrosis due to lung alveolar epithelial cell damage. Tapak liman leaves (Elephantopus scaber L.) have anti-inflammatory and anti-asthmatic properties. This study aimed to investigate the effects of the Elephantop...
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University of Brawijaya
2024-02-01
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| Series: | Journal of Experimental Life Science |
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| Online Access: | https://jels.ub.ac.id/index.php/jels/article/view/554 |
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| author | Sarah Nahdah Nabilah Yuyun Ika Christina Dinia Rizqi Dwijayanti Muhaimin Rifa’i Muhammad Sasmito Djati |
| author_facet | Sarah Nahdah Nabilah Yuyun Ika Christina Dinia Rizqi Dwijayanti Muhaimin Rifa’i Muhammad Sasmito Djati |
| author_sort | Sarah Nahdah Nabilah |
| collection | DOAJ |
| description | IL-6 and IL-17 are pro-inflammatory and pro-fibrotic cytokines that increase pulmonary fibrosis due to lung alveolar epithelial cell damage. Tapak liman leaves (Elephantopus scaber L.) have anti-inflammatory and anti-asthmatic properties. This study aimed to investigate the effects of the Elephantopus scaber L. ethanol extract (ESEE) on IL-6 and IL-17 produced by CD4+ and CD8+ in the bleomycin-induced pulmonary fibrosis mice model. Fifty-six male BALB/c mice will be divided into seven groups consisting of healthy mice (N), vehicle mice (V), pulmonary fibrosis (PF), Dexamethasone (DEX) as a drug control, and three doses of ESEE (0.0504, 0.1008, and 0.2016 mg.kg-1 BW). ESEE will be administered orally, followed by intraperitoneal bleomycin injection for 14 days. Mice are then dissected on days 7 and 14, and the spleen will be isolated for analysis of the expression of IL-6 and IL-17. The results showed that ESEE effectively reduced levels of IL-6 and IL-17 cytokines produced by CD4+ and CD8+ T cells, and doses three of ESEE (0.2016 mg.kg-1 BW) (0.2016 mg.kg-1 BW) showed the most effective reduction activity than the Dexamethasone group. The treatment was proven to reduce the expression of IL-6 and IL-17 in mice with a model of pulmonary fibrosis. |
| format | Article |
| id | doaj-art-0543244de78d495c9db54bfacc47e34e |
| institution | DOAJ |
| issn | 2087-2852 2338-1655 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Experimental Life Science |
| spelling | doaj-art-0543244de78d495c9db54bfacc47e34e2025-08-20T03:19:17ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552024-02-011411017https://doi.org/10.21776/ub.jels.2024.014.01.02Tapak Liman (Elephantopus scaber L.) Leaves Ethanol Extract Improves the Production of IL-6 and IL-17 Cytokines in Mice with Bleomycin-induced Pulmonary FibrosisSarah Nahdah Nabilah0Yuyun Ika Christina1Dinia Rizqi Dwijayanti2Muhaimin Rifa’i3Muhammad Sasmito Djati4Universitas Brawijaya, Malang, IndonesiaUniversitas Brawijaya, Malang, IndonesiaUniversitas Brawijaya, Malang, IndonesiaUniversitas Brawijaya, Malang, IndonesiaUniversitas Brawijaya, Malang, IndonesiaIL-6 and IL-17 are pro-inflammatory and pro-fibrotic cytokines that increase pulmonary fibrosis due to lung alveolar epithelial cell damage. Tapak liman leaves (Elephantopus scaber L.) have anti-inflammatory and anti-asthmatic properties. This study aimed to investigate the effects of the Elephantopus scaber L. ethanol extract (ESEE) on IL-6 and IL-17 produced by CD4+ and CD8+ in the bleomycin-induced pulmonary fibrosis mice model. Fifty-six male BALB/c mice will be divided into seven groups consisting of healthy mice (N), vehicle mice (V), pulmonary fibrosis (PF), Dexamethasone (DEX) as a drug control, and three doses of ESEE (0.0504, 0.1008, and 0.2016 mg.kg-1 BW). ESEE will be administered orally, followed by intraperitoneal bleomycin injection for 14 days. Mice are then dissected on days 7 and 14, and the spleen will be isolated for analysis of the expression of IL-6 and IL-17. The results showed that ESEE effectively reduced levels of IL-6 and IL-17 cytokines produced by CD4+ and CD8+ T cells, and doses three of ESEE (0.2016 mg.kg-1 BW) (0.2016 mg.kg-1 BW) showed the most effective reduction activity than the Dexamethasone group. The treatment was proven to reduce the expression of IL-6 and IL-17 in mice with a model of pulmonary fibrosis.https://jels.ub.ac.id/index.php/jels/article/view/554bleomycinelephantopus scaber l.il-6il-17pulmonary fibrosis. |
| spellingShingle | Sarah Nahdah Nabilah Yuyun Ika Christina Dinia Rizqi Dwijayanti Muhaimin Rifa’i Muhammad Sasmito Djati Tapak Liman (Elephantopus scaber L.) Leaves Ethanol Extract Improves the Production of IL-6 and IL-17 Cytokines in Mice with Bleomycin-induced Pulmonary Fibrosis Journal of Experimental Life Science bleomycin elephantopus scaber l. il-6 il-17 pulmonary fibrosis. |
| title | Tapak Liman (Elephantopus scaber L.) Leaves Ethanol Extract Improves the Production of IL-6 and IL-17 Cytokines in Mice with Bleomycin-induced Pulmonary Fibrosis |
| title_full | Tapak Liman (Elephantopus scaber L.) Leaves Ethanol Extract Improves the Production of IL-6 and IL-17 Cytokines in Mice with Bleomycin-induced Pulmonary Fibrosis |
| title_fullStr | Tapak Liman (Elephantopus scaber L.) Leaves Ethanol Extract Improves the Production of IL-6 and IL-17 Cytokines in Mice with Bleomycin-induced Pulmonary Fibrosis |
| title_full_unstemmed | Tapak Liman (Elephantopus scaber L.) Leaves Ethanol Extract Improves the Production of IL-6 and IL-17 Cytokines in Mice with Bleomycin-induced Pulmonary Fibrosis |
| title_short | Tapak Liman (Elephantopus scaber L.) Leaves Ethanol Extract Improves the Production of IL-6 and IL-17 Cytokines in Mice with Bleomycin-induced Pulmonary Fibrosis |
| title_sort | tapak liman elephantopus scaber l leaves ethanol extract improves the production of il 6 and il 17 cytokines in mice with bleomycin induced pulmonary fibrosis |
| topic | bleomycin elephantopus scaber l. il-6 il-17 pulmonary fibrosis. |
| url | https://jels.ub.ac.id/index.php/jels/article/view/554 |
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