Methylation-Specific PCR Unraveled
Methylation‐specific PCR (MSP) is a simple, quick and cost‐effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whe...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2004-01-01
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| Series: | Cellular Oncology |
| Online Access: | http://dx.doi.org/10.1155/2004/370301 |
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| author | Sarah Derks Marjolein H. F. M. Lentjes Debby M. E. I. Hellebrekers Adriaan P. de Bruïne James G. Herman Manon van Engeland |
| author_facet | Sarah Derks Marjolein H. F. M. Lentjes Debby M. E. I. Hellebrekers Adriaan P. de Bruïne James G. Herman Manon van Engeland |
| author_sort | Sarah Derks |
| collection | DOAJ |
| description | Methylation‐specific PCR (MSP) is a simple, quick and cost‐effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whereby methylated cytosines remains unchanged and (2) detection of the bisulfite induced sequence differences by PCR using specific primer sets for both unmethylated and methylated DNA. This review discusses the critical parameters of MSP and presents an overview of the available MSP variants and the (clinical) applications. |
| format | Article |
| id | doaj-art-05403afa329742dbb10b5ee058bf2c7e |
| institution | OA Journals |
| issn | 1570-5870 1875-8606 |
| language | English |
| publishDate | 2004-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cellular Oncology |
| spelling | doaj-art-05403afa329742dbb10b5ee058bf2c7e2025-08-20T02:02:59ZengWileyCellular Oncology1570-58701875-86062004-01-01265-629129910.1155/2004/370301Methylation-Specific PCR UnraveledSarah Derks0Marjolein H. F. M. Lentjes1Debby M. E. I. Hellebrekers2Adriaan P. de Bruïne3James G. Herman4Manon van Engeland5Research Institute GROW, Department of Pathology, University Maastricht, PO Box 616, 6200 MD Maastricht, The NetherlandsResearch Institute GROW, Department of Pathology, University Maastricht, PO Box 616, 6200 MD Maastricht, The NetherlandsResearch Institute GROW, Department of Pathology, University Maastricht, PO Box 616, 6200 MD Maastricht, The NetherlandsResearch Institute GROW, Department of Pathology, University Maastricht, PO Box 616, 6200 MD Maastricht, The NetherlandsThe Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD 21231, USAResearch Institute GROW, Department of Pathology, University Maastricht, PO Box 616, 6200 MD Maastricht, The NetherlandsMethylation‐specific PCR (MSP) is a simple, quick and cost‐effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whereby methylated cytosines remains unchanged and (2) detection of the bisulfite induced sequence differences by PCR using specific primer sets for both unmethylated and methylated DNA. This review discusses the critical parameters of MSP and presents an overview of the available MSP variants and the (clinical) applications.http://dx.doi.org/10.1155/2004/370301 |
| spellingShingle | Sarah Derks Marjolein H. F. M. Lentjes Debby M. E. I. Hellebrekers Adriaan P. de Bruïne James G. Herman Manon van Engeland Methylation-Specific PCR Unraveled Cellular Oncology |
| title | Methylation-Specific PCR Unraveled |
| title_full | Methylation-Specific PCR Unraveled |
| title_fullStr | Methylation-Specific PCR Unraveled |
| title_full_unstemmed | Methylation-Specific PCR Unraveled |
| title_short | Methylation-Specific PCR Unraveled |
| title_sort | methylation specific pcr unraveled |
| url | http://dx.doi.org/10.1155/2004/370301 |
| work_keys_str_mv | AT sarahderks methylationspecificpcrunraveled AT marjoleinhfmlentjes methylationspecificpcrunraveled AT debbymeihellebrekers methylationspecificpcrunraveled AT adriaanpdebruine methylationspecificpcrunraveled AT jamesgherman methylationspecificpcrunraveled AT manonvanengeland methylationspecificpcrunraveled |