Proliferation and gene expression data of rabbit Achilles tenocyte in vitro culture in the presence of high-molecular weight hyaluronic acid (1.01–1.8 MDa)Mendeley DataMendeley Data
Hyaluronic acid (HA) is a natural bio macromolecule and a good bio lubricant. As it is a predominant component of the synovial fluid, the aim was to explore the effects HA on rabbit Achilles tenocytes in vitro. The data set presents proliferation assessed by the Alamar Blue® assay; and gene expressi...
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Elsevier
2025-06-01
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| Series: | Data in Brief |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S235234092500191X |
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| author | Iris Miescher Petra Wolint Christine Opelz Jess G. Snedeker Pietro Giovanoli Maurizio Calcagni Johanna Buschmann |
| author_facet | Iris Miescher Petra Wolint Christine Opelz Jess G. Snedeker Pietro Giovanoli Maurizio Calcagni Johanna Buschmann |
| author_sort | Iris Miescher |
| collection | DOAJ |
| description | Hyaluronic acid (HA) is a natural bio macromolecule and a good bio lubricant. As it is a predominant component of the synovial fluid, the aim was to explore the effects HA on rabbit Achilles tenocytes in vitro. The data set presents proliferation assessed by the Alamar Blue® assay; and gene expression of tenocytes that were cultured either with or without supplementation of high-molecular weight HA in a concentration of 1.4 mg/mL. There was a daily medium exchange with fresh HA supplementation. On days 3, 7 and 14 the following target genes were analysed and manifold induction was calculated against the control (no HA): Biglycan, Decorin, Aggrecan, Collagen 1A1, Collagen 1A2, Collagen 3, LOX, IL-6, TNF-α, PAR-2, ALOX-15, Tenascin-C, Tenomodulin, Mohawk, α-SMA, TIMP-1, MMP-2, MMP-9, and ki67. These data might be interesting for researchers working in the tendon field. |
| format | Article |
| id | doaj-art-053fb28dd2c64f4eacaab0e4efd9d636 |
| institution | DOAJ |
| issn | 2352-3409 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj-art-053fb28dd2c64f4eacaab0e4efd9d6362025-08-20T03:10:24ZengElsevierData in Brief2352-34092025-06-016011145910.1016/j.dib.2025.111459Proliferation and gene expression data of rabbit Achilles tenocyte in vitro culture in the presence of high-molecular weight hyaluronic acid (1.01–1.8 MDa)Mendeley DataMendeley DataIris Miescher0Petra Wolint1Christine Opelz2Jess G. Snedeker3Pietro Giovanoli4Maurizio Calcagni5Johanna Buschmann6Division of Plastic Surgery and Hand Surgery, University Hospital Zurich, Sternwartstrasse 14, 8091 Zurich, SwitzerlandDivision of Plastic Surgery and Hand Surgery, University Hospital Zurich, Sternwartstrasse 14, 8091 Zurich, SwitzerlandDivision of Plastic Surgery and Hand Surgery, University Hospital Zurich, Sternwartstrasse 14, 8091 Zurich, SwitzerlandUniversity Clinic Balgrist, Orthopaedic Biomechanics, Forchstrasse 340, 8008 Zurich, SwitzerlandDivision of Plastic Surgery and Hand Surgery, University Hospital Zurich, Sternwartstrasse 14, 8091 Zurich, SwitzerlandDivision of Plastic Surgery and Hand Surgery, University Hospital Zurich, Sternwartstrasse 14, 8091 Zurich, SwitzerlandDivision of Plastic Surgery and Hand Surgery, University Hospital Zurich, Sternwartstrasse 14, 8091 Zurich, Switzerland; Corresponding author.Hyaluronic acid (HA) is a natural bio macromolecule and a good bio lubricant. As it is a predominant component of the synovial fluid, the aim was to explore the effects HA on rabbit Achilles tenocytes in vitro. The data set presents proliferation assessed by the Alamar Blue® assay; and gene expression of tenocytes that were cultured either with or without supplementation of high-molecular weight HA in a concentration of 1.4 mg/mL. There was a daily medium exchange with fresh HA supplementation. On days 3, 7 and 14 the following target genes were analysed and manifold induction was calculated against the control (no HA): Biglycan, Decorin, Aggrecan, Collagen 1A1, Collagen 1A2, Collagen 3, LOX, IL-6, TNF-α, PAR-2, ALOX-15, Tenascin-C, Tenomodulin, Mohawk, α-SMA, TIMP-1, MMP-2, MMP-9, and ki67. These data might be interesting for researchers working in the tendon field.http://www.sciencedirect.com/science/article/pii/S235234092500191XDoubling time, high-molecular weight hyaluronic acidBiglycanDecorinAggrecanCollagen 1A1Collagen 1A2 |
| spellingShingle | Iris Miescher Petra Wolint Christine Opelz Jess G. Snedeker Pietro Giovanoli Maurizio Calcagni Johanna Buschmann Proliferation and gene expression data of rabbit Achilles tenocyte in vitro culture in the presence of high-molecular weight hyaluronic acid (1.01–1.8 MDa)Mendeley DataMendeley Data Data in Brief Doubling time, high-molecular weight hyaluronic acid Biglycan Decorin Aggrecan Collagen 1A1 Collagen 1A2 |
| title | Proliferation and gene expression data of rabbit Achilles tenocyte in vitro culture in the presence of high-molecular weight hyaluronic acid (1.01–1.8 MDa)Mendeley DataMendeley Data |
| title_full | Proliferation and gene expression data of rabbit Achilles tenocyte in vitro culture in the presence of high-molecular weight hyaluronic acid (1.01–1.8 MDa)Mendeley DataMendeley Data |
| title_fullStr | Proliferation and gene expression data of rabbit Achilles tenocyte in vitro culture in the presence of high-molecular weight hyaluronic acid (1.01–1.8 MDa)Mendeley DataMendeley Data |
| title_full_unstemmed | Proliferation and gene expression data of rabbit Achilles tenocyte in vitro culture in the presence of high-molecular weight hyaluronic acid (1.01–1.8 MDa)Mendeley DataMendeley Data |
| title_short | Proliferation and gene expression data of rabbit Achilles tenocyte in vitro culture in the presence of high-molecular weight hyaluronic acid (1.01–1.8 MDa)Mendeley DataMendeley Data |
| title_sort | proliferation and gene expression data of rabbit achilles tenocyte in vitro culture in the presence of high molecular weight hyaluronic acid 1 01 1 8 mda mendeley datamendeley data |
| topic | Doubling time, high-molecular weight hyaluronic acid Biglycan Decorin Aggrecan Collagen 1A1 Collagen 1A2 |
| url | http://www.sciencedirect.com/science/article/pii/S235234092500191X |
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