Properties of new polycationic bacteriochlorin photosensitizers: cytotoxicity and interaction with biofilms
Aim: Biofilms of pathogenic bacteria are phenotypically resistant to antibiotics and other antimicrobial agents, host immune systems, and adverse environmental conditions, and are responsible for most chronic infections. Antimicrobial photodynamic therapy (APDT) is based on using of photosensitizers...
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Open Exploration
2024-09-01
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| Series: | Exploration of Drug Science |
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| Online Access: | https://www.explorationpub.com/uploads/Article/A100865/100865.pdf |
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| author | Irina Tiganova Yulia Zhizhimova Eteri Tolordava Elena Makarova Natalia Shevlyagina Alexander Barmashov Maria Baryshnikova Vladimir Zhukhovitsky Yulia Romanova |
| author_facet | Irina Tiganova Yulia Zhizhimova Eteri Tolordava Elena Makarova Natalia Shevlyagina Alexander Barmashov Maria Baryshnikova Vladimir Zhukhovitsky Yulia Romanova |
| author_sort | Irina Tiganova |
| collection | DOAJ |
| description | Aim: Biofilms of pathogenic bacteria are phenotypically resistant to antibiotics and other antimicrobial agents, host immune systems, and adverse environmental conditions, and are responsible for most chronic infections. Antimicrobial photodynamic therapy (APDT) is based on using of photosensitizers (PS), which generate reactive oxygen species when exposed to visible or infrared light. Previously, we demonstrated the high efficacy of new polycationic bacteriochlorins as PS against biofilms of Gram-negative bacteria Pseudomonas aeruginosa in vitro. Now, we compared the toxicity of these PS to bacteria and human fibroblasts, under irradiation and in the dark. Another goal was to study the interaction of the PS with the biofilm matrix without irradiation. Methods: Photodynamic inactivation of eucaryotic cells was obtained with MTT test, and with plating of planktonic bacteria P. aeruginosa and biofilms after disrupting. The interaction of PS with the matrix was studied with electron microscopy, alcian blue staining, and quantitative evaluation of polysaccharides. Results: The absence of dark toxicity of polycationic bacteriochlorins BCl-6 and BCl-7 for human fibroblasts WI-38 at concentrations up to 200 µM and the selectivity of the studied PS to P. aeruginosa 32 bacteria, including these in biofilms, under irradiation, were found. After incubation of P. aeruginosa 32 biofilms with tetracationic BCl-6 and BCl-5 without irradiation, gross disturbances in the structure of the biofilm matrix were observed by SEM, as well as a significant reduction of Alcian blue staining and polysaccharides in the matrix. Conclusions: Polycationic bacteriochlorins BCl-6 and BCl-7 had no dark toxicity for human fibroblasts and were selective to P. aeruginosa 32 bacteria, including these in biofilms, under irradiation. The incubation of biofilms of Gram-negative bacteria with polycationic bacteriochlorin led to the destruction of the matrix without irradiation. |
| format | Article |
| id | doaj-art-053cf168b07a4a58b9726be9712e518b |
| institution | Kabale University |
| issn | 2836-7677 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Open Exploration |
| record_format | Article |
| series | Exploration of Drug Science |
| spelling | doaj-art-053cf168b07a4a58b9726be9712e518b2025-02-08T03:29:23ZengOpen ExplorationExploration of Drug Science2836-76772024-09-012663264710.37349/eds.2024.00065Properties of new polycationic bacteriochlorin photosensitizers: cytotoxicity and interaction with biofilmsIrina Tiganova0https://orcid.org/0000-0003-2340-2726Yulia Zhizhimova1https://orcid.org/0000-0002-0665-9821Eteri Tolordava2https://orcid.org/0000-0002-9920-2432Elena Makarova3https://orcid.org/0000-0003-4144-6159Natalia Shevlyagina4https://orcid.org/0000-0001-9651-1654Alexander Barmashov5https://orcid.org/0000-0002-7440-6404Maria Baryshnikova6https://orcid.org/0000-0002-6688-8423Vladimir Zhukhovitsky7https://orcid.org/0000-0002-4653-2446Yulia Romanova8https://orcid.org/0000-0002-8547-1711Department of Genetics and Molecular Biology of Bacteria, N.F. Gamaleya National Research Center of Epidemiology and Microbiology, 123098 Moscow, RussiaDepartment of Genetics and Molecular Biology of Bacteria, N.F. Gamaleya National Research Center of Epidemiology and Microbiology, 123098 Moscow, RussiaDepartment of Genetics and Molecular Biology of Bacteria, N.F. Gamaleya National Research Center of Epidemiology and Microbiology, 123098 Moscow, RussiaOrganic Intermediates and Dyes Institute, 123001 Moscow, RussiaDepartment of Genetics and Molecular Biology of Bacteria, N.F. Gamaleya National Research Center of Epidemiology and Microbiology, 123098 Moscow, RussiaLaboratory of Experimental Diagnostics and Biotherapy of Cancer, N.N. Blokhin National Medical Research Center of Oncology, 115522 Moscow, RussiaLaboratory of Experimental Diagnostics and Biotherapy of Cancer, N.N. Blokhin National Medical Research Center of Oncology, 115522 Moscow, RussiaDepartment of Genetics and Molecular Biology of Bacteria, N.F. Gamaleya National Research Center of Epidemiology and Microbiology, 123098 Moscow, Russia; Department of Clinical Laboratory Diagnostics, Russian Medical Academy of Continuing Professional Education (RMANPO), Ministry of Public Health of the Russian Federation, 125993 Moscow, RussiaDepartment of Genetics and Molecular Biology of Bacteria, N.F. Gamaleya National Research Center of Epidemiology and Microbiology, 123098 Moscow, RussiaAim: Biofilms of pathogenic bacteria are phenotypically resistant to antibiotics and other antimicrobial agents, host immune systems, and adverse environmental conditions, and are responsible for most chronic infections. Antimicrobial photodynamic therapy (APDT) is based on using of photosensitizers (PS), which generate reactive oxygen species when exposed to visible or infrared light. Previously, we demonstrated the high efficacy of new polycationic bacteriochlorins as PS against biofilms of Gram-negative bacteria Pseudomonas aeruginosa in vitro. Now, we compared the toxicity of these PS to bacteria and human fibroblasts, under irradiation and in the dark. Another goal was to study the interaction of the PS with the biofilm matrix without irradiation. Methods: Photodynamic inactivation of eucaryotic cells was obtained with MTT test, and with plating of planktonic bacteria P. aeruginosa and biofilms after disrupting. The interaction of PS with the matrix was studied with electron microscopy, alcian blue staining, and quantitative evaluation of polysaccharides. Results: The absence of dark toxicity of polycationic bacteriochlorins BCl-6 and BCl-7 for human fibroblasts WI-38 at concentrations up to 200 µM and the selectivity of the studied PS to P. aeruginosa 32 bacteria, including these in biofilms, under irradiation, were found. After incubation of P. aeruginosa 32 biofilms with tetracationic BCl-6 and BCl-5 without irradiation, gross disturbances in the structure of the biofilm matrix were observed by SEM, as well as a significant reduction of Alcian blue staining and polysaccharides in the matrix. Conclusions: Polycationic bacteriochlorins BCl-6 and BCl-7 had no dark toxicity for human fibroblasts and were selective to P. aeruginosa 32 bacteria, including these in biofilms, under irradiation. The incubation of biofilms of Gram-negative bacteria with polycationic bacteriochlorin led to the destruction of the matrix without irradiation.https://www.explorationpub.com/uploads/Article/A100865/100865.pdfphotosensitizerphotodynamic inactivationpolycationic bacteriochlorinhuman fibroblastsbiofilmspseudomonas aeruginosapolysaccharides |
| spellingShingle | Irina Tiganova Yulia Zhizhimova Eteri Tolordava Elena Makarova Natalia Shevlyagina Alexander Barmashov Maria Baryshnikova Vladimir Zhukhovitsky Yulia Romanova Properties of new polycationic bacteriochlorin photosensitizers: cytotoxicity and interaction with biofilms Exploration of Drug Science photosensitizer photodynamic inactivation polycationic bacteriochlorin human fibroblasts biofilms pseudomonas aeruginosa polysaccharides |
| title | Properties of new polycationic bacteriochlorin photosensitizers: cytotoxicity and interaction with biofilms |
| title_full | Properties of new polycationic bacteriochlorin photosensitizers: cytotoxicity and interaction with biofilms |
| title_fullStr | Properties of new polycationic bacteriochlorin photosensitizers: cytotoxicity and interaction with biofilms |
| title_full_unstemmed | Properties of new polycationic bacteriochlorin photosensitizers: cytotoxicity and interaction with biofilms |
| title_short | Properties of new polycationic bacteriochlorin photosensitizers: cytotoxicity and interaction with biofilms |
| title_sort | properties of new polycationic bacteriochlorin photosensitizers cytotoxicity and interaction with biofilms |
| topic | photosensitizer photodynamic inactivation polycationic bacteriochlorin human fibroblasts biofilms pseudomonas aeruginosa polysaccharides |
| url | https://www.explorationpub.com/uploads/Article/A100865/100865.pdf |
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