Genetic identification of F1 and post-F1 serrasalmid juvenile hybrids in Brazilian aquaculture.

Juvenile fish trade monitoring is an important task on Brazilian fish farms. However, the identification of juvenile fish through morphological analysis is not feasible, particularly between interspecific hybrids and pure species individuals, making the monitoring of these individuals difficult. Hyb...

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Main Authors: Diogo Teruo Hashimoto, José Augusto Senhorini, Fausto Foresti, Paulino Martínez, Fábio Porto-Foresti
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089902&type=printable
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author Diogo Teruo Hashimoto
José Augusto Senhorini
Fausto Foresti
Paulino Martínez
Fábio Porto-Foresti
author_facet Diogo Teruo Hashimoto
José Augusto Senhorini
Fausto Foresti
Paulino Martínez
Fábio Porto-Foresti
author_sort Diogo Teruo Hashimoto
collection DOAJ
description Juvenile fish trade monitoring is an important task on Brazilian fish farms. However, the identification of juvenile fish through morphological analysis is not feasible, particularly between interspecific hybrids and pure species individuals, making the monitoring of these individuals difficult. Hybrids can be erroneously identified as pure species in breeding facilities, which might reduce production on farms and negatively affect native populations due to escapes or stocking practices. In the present study, we used a multi-approach analysis (molecular and cytogenetic markers) to identify juveniles of three serrasalmid species (Colossoma macropomum, Piaractus mesopotamicus and Piaractus brachypomus) and their hybrids in different stocks purchased from three seed producers in Brazil. The main findings of this study were the detection of intergenus backcrossing between the hybrid ♀ patinga (P. mesopotamicus×P. brachypomus)×♂ C. macropomum and the occurrence of one hybrid triploid individual. This atypical specimen might result from automixis, a mechanism that produces unreduced gametes in some organisms. Moreover, molecular identification indicated that hybrid individuals are traded as pure species or other types of interspecific hybrids, particularly post-F1 individuals. These results show that serrasalmid fish genomes exhibit high genetic heterogeneity, and multi-approach methods and regulators could improve the surveillance of the production and trade of fish species and their hybrids, thereby facilitating the sustainable development of fish farming.
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spelling doaj-art-052e4b9c136348d98a4d5f2facdea63e2025-08-20T03:11:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0193e8990210.1371/journal.pone.0089902Genetic identification of F1 and post-F1 serrasalmid juvenile hybrids in Brazilian aquaculture.Diogo Teruo HashimotoJosé Augusto SenhoriniFausto ForestiPaulino MartínezFábio Porto-ForestiJuvenile fish trade monitoring is an important task on Brazilian fish farms. However, the identification of juvenile fish through morphological analysis is not feasible, particularly between interspecific hybrids and pure species individuals, making the monitoring of these individuals difficult. Hybrids can be erroneously identified as pure species in breeding facilities, which might reduce production on farms and negatively affect native populations due to escapes or stocking practices. In the present study, we used a multi-approach analysis (molecular and cytogenetic markers) to identify juveniles of three serrasalmid species (Colossoma macropomum, Piaractus mesopotamicus and Piaractus brachypomus) and their hybrids in different stocks purchased from three seed producers in Brazil. The main findings of this study were the detection of intergenus backcrossing between the hybrid ♀ patinga (P. mesopotamicus×P. brachypomus)×♂ C. macropomum and the occurrence of one hybrid triploid individual. This atypical specimen might result from automixis, a mechanism that produces unreduced gametes in some organisms. Moreover, molecular identification indicated that hybrid individuals are traded as pure species or other types of interspecific hybrids, particularly post-F1 individuals. These results show that serrasalmid fish genomes exhibit high genetic heterogeneity, and multi-approach methods and regulators could improve the surveillance of the production and trade of fish species and their hybrids, thereby facilitating the sustainable development of fish farming.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089902&type=printable
spellingShingle Diogo Teruo Hashimoto
José Augusto Senhorini
Fausto Foresti
Paulino Martínez
Fábio Porto-Foresti
Genetic identification of F1 and post-F1 serrasalmid juvenile hybrids in Brazilian aquaculture.
PLoS ONE
title Genetic identification of F1 and post-F1 serrasalmid juvenile hybrids in Brazilian aquaculture.
title_full Genetic identification of F1 and post-F1 serrasalmid juvenile hybrids in Brazilian aquaculture.
title_fullStr Genetic identification of F1 and post-F1 serrasalmid juvenile hybrids in Brazilian aquaculture.
title_full_unstemmed Genetic identification of F1 and post-F1 serrasalmid juvenile hybrids in Brazilian aquaculture.
title_short Genetic identification of F1 and post-F1 serrasalmid juvenile hybrids in Brazilian aquaculture.
title_sort genetic identification of f1 and post f1 serrasalmid juvenile hybrids in brazilian aquaculture
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089902&type=printable
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