Assessment of cfDNA Levels in Saliva Samples of Stressed Young Adults: Preliminary Study
Purpose: The aim of this pilot study was to examine the levels of cell-free DNA (cfDNA) in the saliva samples of second-year medical students before and after a stress-inducing event, exams. Materials and Methods: Saliva samples were collected from 20 students, whose stress profiles were assessed...
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| Format: | Article |
| Language: | English |
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Kirsehir Ahi Evran University
2025-04-01
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| Series: | Ahi Evran Medical Journal |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/4105843 |
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| author | Ekin Çelik Miraç Berkay Köksal Hacı Mehmet Akbulut Selahattin Ata Aydın Yiğit Emre Kurt Can Okan Simay Çetinkaya |
| author_facet | Ekin Çelik Miraç Berkay Köksal Hacı Mehmet Akbulut Selahattin Ata Aydın Yiğit Emre Kurt Can Okan Simay Çetinkaya |
| author_sort | Ekin Çelik |
| collection | DOAJ |
| description | Purpose: The aim of this pilot study was to examine the levels of cell-free DNA (cfDNA) in the saliva samples of second-year medical students before and after a stress-inducing event, exams.
Materials and Methods: Saliva samples were collected from 20 students, whose stress profiles were assessed using the DASS-21 scale, both before and after the exams. Cell-free DNA was isolated from these samples, and measured using spectrophotometry and automated electrophoresis system.
Results: Spectrophotometric analysis revealed a significant decrease in cfDNA levels in the saliva samples collected after the exams (p≤0.05). This difference was confirmed by the automated electrophoresis system, particularly showing a reduction in cfDNA amounts in the 40-200 bp range with the reduction of stress (p≤0.05).
Conclusion: Psychosocial stress affects the release of cell-free DNA. This study reports that the amount of cfDNA found in saliva samples significantly changes in relation to stress. This preliminary study suggests that cfDNA in saliva samples could potentially serve as a biological marker for stress in healthy individuals. |
| format | Article |
| id | doaj-art-052aefc5e65c4efeb6c8bb6e592775e6 |
| institution | Kabale University |
| issn | 2619-9203 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Kirsehir Ahi Evran University |
| record_format | Article |
| series | Ahi Evran Medical Journal |
| spelling | doaj-art-052aefc5e65c4efeb6c8bb6e592775e62025-08-20T03:52:32ZengKirsehir Ahi Evran UniversityAhi Evran Medical Journal2619-92032025-04-0191121610.46332/aemj.1524197Assessment of cfDNA Levels in Saliva Samples of Stressed Young Adults: Preliminary StudyEkin Çelik0https://orcid.org/0000-0003-1966-3907Miraç Berkay Köksal1https://orcid.org/0009-0002-2113-6372Hacı Mehmet Akbulut 2https://orcid.org/0009-0007-8543-9977Selahattin Ata Aydın3https://orcid.org/0009-0009-5525-6015Yiğit Emre Kurt4https://orcid.org/0009-0009-9477-8676Can Okan5https://orcid.org/0009-0006-2499-6502Simay Çetinkaya6https://orcid.org/0000-0002-1182-7754KIRSEHIR AHI EVRAN UNIVERSITYKIRSEHIR AHI EVRAN UNIVERSITYKIRSEHIR AHI EVRAN UNIVERSITYKIRSEHIR AHI EVRAN UNIVERSITYKIRSEHIR AHI EVRAN UNIVERSITYKIRSEHIR AHI EVRAN UNIVERSITYBir kuruma bağlı değildirPurpose: The aim of this pilot study was to examine the levels of cell-free DNA (cfDNA) in the saliva samples of second-year medical students before and after a stress-inducing event, exams. Materials and Methods: Saliva samples were collected from 20 students, whose stress profiles were assessed using the DASS-21 scale, both before and after the exams. Cell-free DNA was isolated from these samples, and measured using spectrophotometry and automated electrophoresis system. Results: Spectrophotometric analysis revealed a significant decrease in cfDNA levels in the saliva samples collected after the exams (p≤0.05). This difference was confirmed by the automated electrophoresis system, particularly showing a reduction in cfDNA amounts in the 40-200 bp range with the reduction of stress (p≤0.05). Conclusion: Psychosocial stress affects the release of cell-free DNA. This study reports that the amount of cfDNA found in saliva samples significantly changes in relation to stress. This preliminary study suggests that cfDNA in saliva samples could potentially serve as a biological marker for stress in healthy individuals.https://dergipark.org.tr/tr/download/article-file/4105843biomarkercfdnaplasma |
| spellingShingle | Ekin Çelik Miraç Berkay Köksal Hacı Mehmet Akbulut Selahattin Ata Aydın Yiğit Emre Kurt Can Okan Simay Çetinkaya Assessment of cfDNA Levels in Saliva Samples of Stressed Young Adults: Preliminary Study Ahi Evran Medical Journal biomarker cfdna plasma |
| title | Assessment of cfDNA Levels in Saliva Samples of Stressed Young Adults: Preliminary Study |
| title_full | Assessment of cfDNA Levels in Saliva Samples of Stressed Young Adults: Preliminary Study |
| title_fullStr | Assessment of cfDNA Levels in Saliva Samples of Stressed Young Adults: Preliminary Study |
| title_full_unstemmed | Assessment of cfDNA Levels in Saliva Samples of Stressed Young Adults: Preliminary Study |
| title_short | Assessment of cfDNA Levels in Saliva Samples of Stressed Young Adults: Preliminary Study |
| title_sort | assessment of cfdna levels in saliva samples of stressed young adults preliminary study |
| topic | biomarker cfdna plasma |
| url | https://dergipark.org.tr/tr/download/article-file/4105843 |
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