FLIM-Phasor Analysis (FLIM-ϕ) of Aβ-Induced Membrane Order Alterations: Towards a Cell-Based Biosensor for Early Alzheimer’s Disease Diagnosis
Alzheimer’s disease (AD) is a progressive neurodegenerative disorder, and its early detection can be critical for a prompt intervention that can potentially slow down the disease progression and improve the patient’s quality of life. However, a diagnosis based solely on clinical symptoms can be chal...
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MDPI AG
2025-02-01
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| Online Access: | https://www.mdpi.com/2072-666X/16/2/234 |
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| author | Antonella Battisti Maria Grazia Ortore Silvia Vilasi Antonella Sgarbossa |
| author_facet | Antonella Battisti Maria Grazia Ortore Silvia Vilasi Antonella Sgarbossa |
| author_sort | Antonella Battisti |
| collection | DOAJ |
| description | Alzheimer’s disease (AD) is a progressive neurodegenerative disorder, and its early detection can be critical for a prompt intervention that can potentially slow down the disease progression and improve the patient’s quality of life. However, a diagnosis based solely on clinical symptoms can be challenging, especially in the early stages, while the detection of specific biomarkers such as amyloid-β peptide (Aβ) and tau proteins can provide objective evidence for diagnosis. In this work, we explored the effects of Aβ peptide on cell membrane properties thanks to fluorescence lifetime imaging (FLIM) combined with the phasor analysis (FLIM-ϕ). The results showed that the membrane viscosity is altered by the presence of Aβ peptide and that cells experience this effect even at nanomolar concentrations of peptide. This considerable sensitivity opens up the possibility of envisioning a cell-based biosensor able to detect very low concentrations of Aβ in a biological fluid, thus enabling timely diagnosis and intervention. |
| format | Article |
| id | doaj-art-0522bcd9e99d4ac3af3756e0d8df6911 |
| institution | OA Journals |
| issn | 2072-666X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj-art-0522bcd9e99d4ac3af3756e0d8df69112025-08-20T02:03:40ZengMDPI AGMicromachines2072-666X2025-02-0116223410.3390/mi16020234FLIM-Phasor Analysis (FLIM-ϕ) of Aβ-Induced Membrane Order Alterations: Towards a Cell-Based Biosensor for Early Alzheimer’s Disease DiagnosisAntonella Battisti0Maria Grazia Ortore1Silvia Vilasi2Antonella Sgarbossa3NEST, Nanoscience Institute-CNR and Scuola Normale Superiore, p.zza San Silvestro 12, I-56127 Pisa, ItalyDipartimento di Scienze Della Vita e dell’Ambiente, Università Politecnica delle Marche, Via Brecce Bianche, I-60131 Ancona, ItalyBiophysics Institute-CNR, Via Ugo La Malfa 153, I-90146 Palermo, ItalyNEST, Nanoscience Institute-CNR and Scuola Normale Superiore, p.zza San Silvestro 12, I-56127 Pisa, ItalyAlzheimer’s disease (AD) is a progressive neurodegenerative disorder, and its early detection can be critical for a prompt intervention that can potentially slow down the disease progression and improve the patient’s quality of life. However, a diagnosis based solely on clinical symptoms can be challenging, especially in the early stages, while the detection of specific biomarkers such as amyloid-β peptide (Aβ) and tau proteins can provide objective evidence for diagnosis. In this work, we explored the effects of Aβ peptide on cell membrane properties thanks to fluorescence lifetime imaging (FLIM) combined with the phasor analysis (FLIM-ϕ). The results showed that the membrane viscosity is altered by the presence of Aβ peptide and that cells experience this effect even at nanomolar concentrations of peptide. This considerable sensitivity opens up the possibility of envisioning a cell-based biosensor able to detect very low concentrations of Aβ in a biological fluid, thus enabling timely diagnosis and intervention.https://www.mdpi.com/2072-666X/16/2/234Alzheimer’s diseasecell-based biosensoramyloid peptide biosensorFLIM-phasor analysisAβ detection |
| spellingShingle | Antonella Battisti Maria Grazia Ortore Silvia Vilasi Antonella Sgarbossa FLIM-Phasor Analysis (FLIM-ϕ) of Aβ-Induced Membrane Order Alterations: Towards a Cell-Based Biosensor for Early Alzheimer’s Disease Diagnosis Micromachines Alzheimer’s disease cell-based biosensor amyloid peptide biosensor FLIM-phasor analysis Aβ detection |
| title | FLIM-Phasor Analysis (FLIM-ϕ) of Aβ-Induced Membrane Order Alterations: Towards a Cell-Based Biosensor for Early Alzheimer’s Disease Diagnosis |
| title_full | FLIM-Phasor Analysis (FLIM-ϕ) of Aβ-Induced Membrane Order Alterations: Towards a Cell-Based Biosensor for Early Alzheimer’s Disease Diagnosis |
| title_fullStr | FLIM-Phasor Analysis (FLIM-ϕ) of Aβ-Induced Membrane Order Alterations: Towards a Cell-Based Biosensor for Early Alzheimer’s Disease Diagnosis |
| title_full_unstemmed | FLIM-Phasor Analysis (FLIM-ϕ) of Aβ-Induced Membrane Order Alterations: Towards a Cell-Based Biosensor for Early Alzheimer’s Disease Diagnosis |
| title_short | FLIM-Phasor Analysis (FLIM-ϕ) of Aβ-Induced Membrane Order Alterations: Towards a Cell-Based Biosensor for Early Alzheimer’s Disease Diagnosis |
| title_sort | flim phasor analysis flim ϕ of aβ induced membrane order alterations towards a cell based biosensor for early alzheimer s disease diagnosis |
| topic | Alzheimer’s disease cell-based biosensor amyloid peptide biosensor FLIM-phasor analysis Aβ detection |
| url | https://www.mdpi.com/2072-666X/16/2/234 |
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