Lime peel essential oil microcapsules in alginate-gelatin for antimicrobial use in Musa spp. micropropagation
Tissue culture micropropagation is the most effective method for propagating Musa spp. explants; however, microbial contamination poses a major obstacle. Lime peel essential oil (LPO) has demonstrated antimicrobial activity against contaminants in Musa spp. cultures, though its effectiveness is limi...
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Elsevier
2025-03-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893924002299 |
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| author | Nandang Permadi Mohamad Nurzaman Febri Doni Euis Julaeha |
| author_facet | Nandang Permadi Mohamad Nurzaman Febri Doni Euis Julaeha |
| author_sort | Nandang Permadi |
| collection | DOAJ |
| description | Tissue culture micropropagation is the most effective method for propagating Musa spp. explants; however, microbial contamination poses a major obstacle. Lime peel essential oil (LPO) has demonstrated antimicrobial activity against contaminants in Musa spp. cultures, though its effectiveness is limited by instability and rapid release. Encapsulation technology using sodium alginate and gelatin biopolymers addresses these limitations by protecting active compounds, enabling controlled release, and extending shelf life. In this study, LPO was encapsulated with a yield of 42.35 %, encapsulation efficiency (EE) of 93.01 %, and oil content (OC) of 76.02 %. The resulting microcapsules were spherical, averaging 1.37 µm in size, and exhibited release kinetics consistent with the Avrami model, confirming delayed LPO release. The microcapsules showed thermal stability up to 100 °C, maintaining 45.75 % OC and 56.16 % EE over three weeks. LPO effectively reduced contamination rates (CR) and improving survival rates (SR) in Kepok Tanjung and Barangan explants. Encapsulated LPO provided moderate CR reductions and SR improvements. Both LPO and its encapsulated form significantly enhanced biomass addition and relative growth rate, with higher concentrations yielding the most pronounced effects. These results indicate that LPO, particularly in its encapsulated form, is effective for promoting growth and reducing contamination in Musa spp. tissue cultures. |
| format | Article |
| id | doaj-art-0513f0a04abb4afda7a4688ab595f7fe |
| institution | OA Journals |
| issn | 2666-8939 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj-art-0513f0a04abb4afda7a4688ab595f7fe2025-08-20T02:06:35ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392025-03-01910064910.1016/j.carpta.2024.100649Lime peel essential oil microcapsules in alginate-gelatin for antimicrobial use in Musa spp. micropropagationNandang Permadi0Mohamad Nurzaman1Febri Doni2Euis Julaeha3Doctorate Program in Biotechnology, Graduate School, Universitas Padjadjaran, Bandung 40132, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia; Corresponding author.Tissue culture micropropagation is the most effective method for propagating Musa spp. explants; however, microbial contamination poses a major obstacle. Lime peel essential oil (LPO) has demonstrated antimicrobial activity against contaminants in Musa spp. cultures, though its effectiveness is limited by instability and rapid release. Encapsulation technology using sodium alginate and gelatin biopolymers addresses these limitations by protecting active compounds, enabling controlled release, and extending shelf life. In this study, LPO was encapsulated with a yield of 42.35 %, encapsulation efficiency (EE) of 93.01 %, and oil content (OC) of 76.02 %. The resulting microcapsules were spherical, averaging 1.37 µm in size, and exhibited release kinetics consistent with the Avrami model, confirming delayed LPO release. The microcapsules showed thermal stability up to 100 °C, maintaining 45.75 % OC and 56.16 % EE over three weeks. LPO effectively reduced contamination rates (CR) and improving survival rates (SR) in Kepok Tanjung and Barangan explants. Encapsulated LPO provided moderate CR reductions and SR improvements. Both LPO and its encapsulated form significantly enhanced biomass addition and relative growth rate, with higher concentrations yielding the most pronounced effects. These results indicate that LPO, particularly in its encapsulated form, is effective for promoting growth and reducing contamination in Musa spp. tissue cultures.http://www.sciencedirect.com/science/article/pii/S2666893924002299Complex coacervationContaminationLime peel essential oilsMicroencapsulationMusa spp. tissue culture |
| spellingShingle | Nandang Permadi Mohamad Nurzaman Febri Doni Euis Julaeha Lime peel essential oil microcapsules in alginate-gelatin for antimicrobial use in Musa spp. micropropagation Carbohydrate Polymer Technologies and Applications Complex coacervation Contamination Lime peel essential oils Microencapsulation Musa spp. tissue culture |
| title | Lime peel essential oil microcapsules in alginate-gelatin for antimicrobial use in Musa spp. micropropagation |
| title_full | Lime peel essential oil microcapsules in alginate-gelatin for antimicrobial use in Musa spp. micropropagation |
| title_fullStr | Lime peel essential oil microcapsules in alginate-gelatin for antimicrobial use in Musa spp. micropropagation |
| title_full_unstemmed | Lime peel essential oil microcapsules in alginate-gelatin for antimicrobial use in Musa spp. micropropagation |
| title_short | Lime peel essential oil microcapsules in alginate-gelatin for antimicrobial use in Musa spp. micropropagation |
| title_sort | lime peel essential oil microcapsules in alginate gelatin for antimicrobial use in musa spp micropropagation |
| topic | Complex coacervation Contamination Lime peel essential oils Microencapsulation Musa spp. tissue culture |
| url | http://www.sciencedirect.com/science/article/pii/S2666893924002299 |
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