Characterization of NGF, trkANGFR, and p75NTR in Retina of Mice Lacking Reelin Glycoprotein
Both Reelin and Nerve Growth Factor (NGF) exert crucial roles in retinal development. Retinogenesis is severely impaired in E-reeler mice, a model of Reelin deficiency showing specific Green Fluorescent Protein expression in Rod Bipolar Cells (RBCs). Since no data are available on Reelin and NGF cro...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Cell Biology |
| Online Access: | http://dx.doi.org/10.1155/2014/725928 |
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| author | Bijorn Omar Balzamino Filippo Biamonte Graziana Esposito Ramona Marino Francesca Fanelli Flavio Keller Alessandra Micera |
| author_facet | Bijorn Omar Balzamino Filippo Biamonte Graziana Esposito Ramona Marino Francesca Fanelli Flavio Keller Alessandra Micera |
| author_sort | Bijorn Omar Balzamino |
| collection | DOAJ |
| description | Both Reelin and Nerve Growth Factor (NGF) exert crucial roles in retinal development. Retinogenesis is severely impaired in E-reeler mice, a model of Reelin deficiency showing specific Green Fluorescent Protein expression in Rod Bipolar Cells (RBCs). Since no data are available on Reelin and NGF cross-talk, NGF and trkANGFR/ p75NTR expression was investigated in retinas from E-reeler versus control mice, by confocal microscopy, Western blotting, and real time PCR analysis. A scattered increase of NGF protein was observed in the Ganglion Cell Layer and more pronounced in the Inner Nuclear Layer (INL). A selective increase of p75NTR was detected in most of RBCs and in other cell subtypes of INL. On the contrary, a slight trend towards a decrease was detected for trkANGFR, albeit not significant. Confocal data were validated by Western blot and real time PCR. Finally, the decreased trkANGFR/ p75NTR ratio, representative of p75NTR increase, significantly correlated with E-reeler versus E-control. These data indicate that NGF-trkANGFR/ p75NTR is affected in E-reeler retina and that p75NTR might represent the main NGF receptor involved in the process. This first NGF-trkANGFR/ p75NTR characterization suggests that E-reeler might be suitable for exploring Reelin-NGF cross-talk, representing an additional information source in those pathologies characterized by retinal degeneration. |
| format | Article |
| id | doaj-art-050af7ff89bd41a2a3d59b5615846c60 |
| institution | Kabale University |
| issn | 1687-8876 1687-8884 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Cell Biology |
| spelling | doaj-art-050af7ff89bd41a2a3d59b5615846c602025-02-03T05:51:48ZengWileyInternational Journal of Cell Biology1687-88761687-88842014-01-01201410.1155/2014/725928725928Characterization of NGF, trkANGFR, and p75NTR in Retina of Mice Lacking Reelin GlycoproteinBijorn Omar Balzamino0Filippo Biamonte1Graziana Esposito2Ramona Marino3Francesca Fanelli4Flavio Keller5Alessandra Micera6IRCCS-G.B. Bietti Foundation, Rome, ItalyInstitute of Histology and Embryology, School of Medicine “A. Gemelli”, Catholic University of the Sacred Heart, Rome, ItalyIRCCS-G.B. Bietti Foundation, Rome, ItalyLaboratory of Developmental Neuroscience and Neural Plasticity, University Campus Bio-Medico, Rome, ItalyLaboratory of Developmental Neuroscience and Neural Plasticity, University Campus Bio-Medico, Rome, ItalyLaboratory of Developmental Neuroscience and Neural Plasticity, University Campus Bio-Medico, Rome, ItalyIRCCS-G.B. Bietti Foundation, Rome, ItalyBoth Reelin and Nerve Growth Factor (NGF) exert crucial roles in retinal development. Retinogenesis is severely impaired in E-reeler mice, a model of Reelin deficiency showing specific Green Fluorescent Protein expression in Rod Bipolar Cells (RBCs). Since no data are available on Reelin and NGF cross-talk, NGF and trkANGFR/ p75NTR expression was investigated in retinas from E-reeler versus control mice, by confocal microscopy, Western blotting, and real time PCR analysis. A scattered increase of NGF protein was observed in the Ganglion Cell Layer and more pronounced in the Inner Nuclear Layer (INL). A selective increase of p75NTR was detected in most of RBCs and in other cell subtypes of INL. On the contrary, a slight trend towards a decrease was detected for trkANGFR, albeit not significant. Confocal data were validated by Western blot and real time PCR. Finally, the decreased trkANGFR/ p75NTR ratio, representative of p75NTR increase, significantly correlated with E-reeler versus E-control. These data indicate that NGF-trkANGFR/ p75NTR is affected in E-reeler retina and that p75NTR might represent the main NGF receptor involved in the process. This first NGF-trkANGFR/ p75NTR characterization suggests that E-reeler might be suitable for exploring Reelin-NGF cross-talk, representing an additional information source in those pathologies characterized by retinal degeneration.http://dx.doi.org/10.1155/2014/725928 |
| spellingShingle | Bijorn Omar Balzamino Filippo Biamonte Graziana Esposito Ramona Marino Francesca Fanelli Flavio Keller Alessandra Micera Characterization of NGF, trkANGFR, and p75NTR in Retina of Mice Lacking Reelin Glycoprotein International Journal of Cell Biology |
| title | Characterization of NGF, trkANGFR, and p75NTR in Retina of Mice Lacking Reelin Glycoprotein |
| title_full | Characterization of NGF, trkANGFR, and p75NTR in Retina of Mice Lacking Reelin Glycoprotein |
| title_fullStr | Characterization of NGF, trkANGFR, and p75NTR in Retina of Mice Lacking Reelin Glycoprotein |
| title_full_unstemmed | Characterization of NGF, trkANGFR, and p75NTR in Retina of Mice Lacking Reelin Glycoprotein |
| title_short | Characterization of NGF, trkANGFR, and p75NTR in Retina of Mice Lacking Reelin Glycoprotein |
| title_sort | characterization of ngf trkangfr and p75ntr in retina of mice lacking reelin glycoprotein |
| url | http://dx.doi.org/10.1155/2014/725928 |
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