Integrated multi-omics analysis unravels the floral scent characteristics and regulation in “Hutou” multi-petal jasmine
Abstract The multi-petal “Hutou” jasmine (Jasminum sambac var. Trifoliatum) is highly valued for bonsai cultivation and landscape design, however, the aroma profile and mechanisms underlying floral scent formation remain elusive. In this study, we generate a nearly complete telomere-to-telomere (T2T...
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Nature Portfolio
2025-02-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-07685-w |
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| author | Jingping Fang Linwei Zhou Qinchang Chen Jinbin Wang Ying Zhuang Shaoqing Lin Hansong Yan Kai Zhao Jisen Zhang Robert J. Henry |
| author_facet | Jingping Fang Linwei Zhou Qinchang Chen Jinbin Wang Ying Zhuang Shaoqing Lin Hansong Yan Kai Zhao Jisen Zhang Robert J. Henry |
| author_sort | Jingping Fang |
| collection | DOAJ |
| description | Abstract The multi-petal “Hutou” jasmine (Jasminum sambac var. Trifoliatum) is highly valued for bonsai cultivation and landscape design, however, the aroma profile and mechanisms underlying floral scent formation remain elusive. In this study, we generate a nearly complete telomere-to-telomere (T2T) genome assembly of “Hutou” jasmine (487.45 Mb with contig N50 of 38.93 Mb). Metabolomic profiling unveils that 16 significantly differential volatiles (SDVs) may play a crucial role in the formation of flower aroma. Among them, five scented SDVs, particularly α-farnesene and pentanoic acid 1-ethenyl-1,5-dimethyl-4-hexenyl ester, contribute to the characteristic aroma profile of “Hutou” jasmine flowers. Weighted gene co-expression network analysis (WGCNA) identifies HTWRKY41, HTWRKY53, and HTHSP90 as the hub genes potentially regulating the production of these 16 metabolites. The expression of selected genes and duplication events drive the increased relative content of major sesquiterpenoids in terpenoid biosynthetic pathway. Four structural genes (BEAT3, BSMT1, BPBT2, and BPBT3) are potentially implicated in the emission of downstream key volatile esters (benzyl acetate, methyl benzoate, and benzyl benzoate) in the phenylpropanoids synthesis. Our integrated dataset of genomics, transcriptomics, and metabolomics present here provides a theoretical basis for the practical utilization of fragrance and genetic improvement in horticultural applications of “Hutou” jasmine. |
| format | Article |
| id | doaj-art-04f4f09df2d84e99b927f5401d0b6727 |
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| issn | 2399-3642 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
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| series | Communications Biology |
| spelling | doaj-art-04f4f09df2d84e99b927f5401d0b67272025-08-20T02:15:06ZengNature PortfolioCommunications Biology2399-36422025-02-018112010.1038/s42003-025-07685-wIntegrated multi-omics analysis unravels the floral scent characteristics and regulation in “Hutou” multi-petal jasmineJingping Fang0Linwei Zhou1Qinchang Chen2Jinbin Wang3Ying Zhuang4Shaoqing Lin5Hansong Yan6Kai Zhao7Jisen Zhang8Robert J. Henry9College of Life Science, Fujian Normal UniversityCollege of Life Science, Fujian Normal UniversityCollege of Life Science, Fujian Normal UniversityCollege of Life Science, Fujian Normal UniversityCollege of Life Science, Fujian Normal UniversityCollege of Life Science, Fujian Normal UniversityCenter for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry UniversityCollege of Life Science, Fujian Normal UniversityCenter for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry UniversityQueensland Alliance for Agriculture and Food Innovation, University of QueenslandAbstract The multi-petal “Hutou” jasmine (Jasminum sambac var. Trifoliatum) is highly valued for bonsai cultivation and landscape design, however, the aroma profile and mechanisms underlying floral scent formation remain elusive. In this study, we generate a nearly complete telomere-to-telomere (T2T) genome assembly of “Hutou” jasmine (487.45 Mb with contig N50 of 38.93 Mb). Metabolomic profiling unveils that 16 significantly differential volatiles (SDVs) may play a crucial role in the formation of flower aroma. Among them, five scented SDVs, particularly α-farnesene and pentanoic acid 1-ethenyl-1,5-dimethyl-4-hexenyl ester, contribute to the characteristic aroma profile of “Hutou” jasmine flowers. Weighted gene co-expression network analysis (WGCNA) identifies HTWRKY41, HTWRKY53, and HTHSP90 as the hub genes potentially regulating the production of these 16 metabolites. The expression of selected genes and duplication events drive the increased relative content of major sesquiterpenoids in terpenoid biosynthetic pathway. Four structural genes (BEAT3, BSMT1, BPBT2, and BPBT3) are potentially implicated in the emission of downstream key volatile esters (benzyl acetate, methyl benzoate, and benzyl benzoate) in the phenylpropanoids synthesis. Our integrated dataset of genomics, transcriptomics, and metabolomics present here provides a theoretical basis for the practical utilization of fragrance and genetic improvement in horticultural applications of “Hutou” jasmine.https://doi.org/10.1038/s42003-025-07685-w |
| spellingShingle | Jingping Fang Linwei Zhou Qinchang Chen Jinbin Wang Ying Zhuang Shaoqing Lin Hansong Yan Kai Zhao Jisen Zhang Robert J. Henry Integrated multi-omics analysis unravels the floral scent characteristics and regulation in “Hutou” multi-petal jasmine Communications Biology |
| title | Integrated multi-omics analysis unravels the floral scent characteristics and regulation in “Hutou” multi-petal jasmine |
| title_full | Integrated multi-omics analysis unravels the floral scent characteristics and regulation in “Hutou” multi-petal jasmine |
| title_fullStr | Integrated multi-omics analysis unravels the floral scent characteristics and regulation in “Hutou” multi-petal jasmine |
| title_full_unstemmed | Integrated multi-omics analysis unravels the floral scent characteristics and regulation in “Hutou” multi-petal jasmine |
| title_short | Integrated multi-omics analysis unravels the floral scent characteristics and regulation in “Hutou” multi-petal jasmine |
| title_sort | integrated multi omics analysis unravels the floral scent characteristics and regulation in hutou multi petal jasmine |
| url | https://doi.org/10.1038/s42003-025-07685-w |
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