Clinical utility of universal 16S rRNA PCR using sterile body fluids: lung abscess caused by Nocardia farcinica
Nocardiosis is an infectious disease caused by Gram-positive rod-shaped bacteria and presents as a suppurative granulomatous disease in patients with compromised immune systems. Few studies have investigated the clinical utility of the universal 16S rRNA polymerase chain reaction (PCR) method using...
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The Journal of Infection in Developing Countries
2023-05-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/15449 |
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| author | Jungseo Park Jun-Won Seo Na Ra Yoon Dong-Min Kim Choon-Mee Kim |
| author_facet | Jungseo Park Jun-Won Seo Na Ra Yoon Dong-Min Kim Choon-Mee Kim |
| author_sort | Jungseo Park |
| collection | DOAJ |
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Nocardiosis is an infectious disease caused by Gram-positive rod-shaped bacteria and presents as a suppurative granulomatous disease in patients with compromised immune systems. Few studies have investigated the clinical utility of the universal 16S rRNA polymerase chain reaction (PCR) method using sterile body fluids for diagnosing nocardiosis.
A 64-year-old female patient was admitted to Chosun University Hospital with the complaint of fever. Computed tomography scans of her chest revealed the presence of empyema and an abscess in the right lung. Pus samples were collected using closed chest thoracostomy and were cultured. The results revealed the presence of Gram-positive bacilli, but the culture tests were unable to identify the causative microorganism. Despite antibiotic treatment, the patient died of the suspected empyema and abscess. Universal 16S PCR of her sterile body fluids in combination with sequencing was performed, which led to the diagnosis of Nocardia farcinica infection. Postmortem, the remainder of the pus samples cultured for 8 days confirmed the presence of N. farcinica. This study illustrates the importance of using routine universal 16S rRNA PCR with sterile body fluids to help diagnose atypical bacterial infections such as nocardiosis.
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| format | Article |
| id | doaj-art-04f4a30e4e7c444e934aeaf1be666d1d |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-04f4a30e4e7c444e934aeaf1be666d1d2025-08-20T03:52:43ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802023-05-01170510.3855/jidc.15449Clinical utility of universal 16S rRNA PCR using sterile body fluids: lung abscess caused by Nocardia farcinicaJungseo Park0Jun-Won Seo1Na Ra Yoon2Dong-Min Kim3Choon-Mee Kim4College of Medicine, Chosun University, Gwangju, South KoreaGraduate School of Chosun University, Gwangju, Republic of KoreaDepartments of Internal Medicine, College of Medicine, Chosun University, Gwangju, Republic of KoreaDepartments of Internal Medicine, College of Medicine, Chosun University, Gwangju, Republic of KoreaPremedical Science, College of Medicine, Chosun University, Gwangju, Republic of Korea Nocardiosis is an infectious disease caused by Gram-positive rod-shaped bacteria and presents as a suppurative granulomatous disease in patients with compromised immune systems. Few studies have investigated the clinical utility of the universal 16S rRNA polymerase chain reaction (PCR) method using sterile body fluids for diagnosing nocardiosis. A 64-year-old female patient was admitted to Chosun University Hospital with the complaint of fever. Computed tomography scans of her chest revealed the presence of empyema and an abscess in the right lung. Pus samples were collected using closed chest thoracostomy and were cultured. The results revealed the presence of Gram-positive bacilli, but the culture tests were unable to identify the causative microorganism. Despite antibiotic treatment, the patient died of the suspected empyema and abscess. Universal 16S PCR of her sterile body fluids in combination with sequencing was performed, which led to the diagnosis of Nocardia farcinica infection. Postmortem, the remainder of the pus samples cultured for 8 days confirmed the presence of N. farcinica. This study illustrates the importance of using routine universal 16S rRNA PCR with sterile body fluids to help diagnose atypical bacterial infections such as nocardiosis. https://jidc.org/index.php/journal/article/view/1544916S rRNAGram-positive bacteriapolymerase chain reactionGram-stainingnocardiosis |
| spellingShingle | Jungseo Park Jun-Won Seo Na Ra Yoon Dong-Min Kim Choon-Mee Kim Clinical utility of universal 16S rRNA PCR using sterile body fluids: lung abscess caused by Nocardia farcinica Journal of Infection in Developing Countries 16S rRNA Gram-positive bacteria polymerase chain reaction Gram-staining nocardiosis |
| title | Clinical utility of universal 16S rRNA PCR using sterile body fluids: lung abscess caused by Nocardia farcinica |
| title_full | Clinical utility of universal 16S rRNA PCR using sterile body fluids: lung abscess caused by Nocardia farcinica |
| title_fullStr | Clinical utility of universal 16S rRNA PCR using sterile body fluids: lung abscess caused by Nocardia farcinica |
| title_full_unstemmed | Clinical utility of universal 16S rRNA PCR using sterile body fluids: lung abscess caused by Nocardia farcinica |
| title_short | Clinical utility of universal 16S rRNA PCR using sterile body fluids: lung abscess caused by Nocardia farcinica |
| title_sort | clinical utility of universal 16s rrna pcr using sterile body fluids lung abscess caused by nocardia farcinica |
| topic | 16S rRNA Gram-positive bacteria polymerase chain reaction Gram-staining nocardiosis |
| url | https://jidc.org/index.php/journal/article/view/15449 |
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